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We narrowed to 612 results for: SCR

Showing: 201 - 250 of 612 results

  1. Advancing Biology with Zebrafish: Genetic Tools for Developmental Studies and More

    Type
    Blog Post
    Published
    Aug. 13, 2019, 12:58 p.m.
    ...sequenced genome also permits “whole-genome/transcriptome” studies, in which, for example, the activity...two revolutionary large-scale random mutagenesis screens produced ~1,500 zebrafish mutants that are still...deactivation tools for zebrafish Random mutagenesis screens provided a major toehold into understanding how...one-eyed pinhead mutant from the random mutagenesis screens. Microscopy-based lineage tracing can help illuminate...

  2. Data Freedom: The Expansion of Data Sharing in Research Publications

    Type
    Blog Post
    Published
    Aug. 5, 2014, 6:51 p.m.
    ...policy and recently announced that of the 16,000 manuscripts that had been processed since the declaration...access of this data is to be made at the time of manuscript submission. The rationale includes increasing...some cases, typically consortia involving genomic screens, the data stream is so prolific that raw information...issue by requiring ever more condensed methods descriptions, legends and limiting numbers of figures such...

  3. Plasmids 101: Expressing shRNAs for RNA Interference

    Type
    Blog Post
    Published
    June 17, 2025, 1:15 p.m.
    ...processing (Figure 1). The shRNA sequence is transcribed to produce hairpin shRNA structures that are ...fact! Endogenous miRNA production starts with transcription of the miRNA to create pri-miRNA, which is then...typically use RNA polymerase III promoters, which transcribe small RNAs. The most common promoters are U6,... H., & Kim, V. N. (2004). MicroRNA genes are transcribed by RNA polymerase II. The EMBO Journal, 23(20...

  4. CRISPR 101: Engineering the Plant Genome Using CRISPR/Cas9

    Type
    Blog Post
    Published
    Oct. 11, 2016, 2:30 p.m.
    ... editor or adenine base editor) and reverse transcriptase-mediated prime editing technologies have been... through Addgene. In addition to the plasmids described above, Addgene carries several useful CRISPR toolkits...Toolbox for Multiplexed Plant Genome Editing and Transcriptional Regulation. Plant Physiol 169:971–985 . https...CRISPR/Cas9-Targeted Mutagenesis of the ERF Transcription Factor Gene OsERF922. PLoS ONE 11:e0154027 ....

  5. Evolution of Lab Techniques

    Type
    Blog Post
    Published
    June 21, 2016, 2:30 p.m.
    ...including easier automation, higher throughput screening, and a lower requirement for the amount of DNA...transgenic animals may choose Southern blotting to screen founder transgenic animals for the inserted transgene...below), restriction mapping may still be used to screen multiple colonies before selecting a smaller subset...prevails. Sequencing whole genomes or profiling transcriptomes (RNAseq) require sequencing of many samples...

  6. Plasmids 101: Dimers and Multimers

    Type
    Blog Post
    Published
    Jan. 10, 2023, 2:15 p.m.
    ...these cases, screening multiple bacterial colonies using one of the detection methods described above can...confirmations like linear and nicked plasmids, as described in this article.   Long-read sequencing Addgene...by growing seed stocks at 30°C compared to 42°C. Screen multiple colonies to isolate the monomer Sometimes...

  7. Easi-CRISPR: Generating Knock-In and Conditional Mouse Models

    Type
    Blog Post
    Published
    April 5, 2018, 12:42 p.m.
    ...perform in vitro transcription, and use the IVT product as a template for reverse transcription to create your...process of mouse model generation simpler, as described in an earlier post on generating CRISPR mouse ...'d use ivTRT to create the ssDNA construct as described in Figure 2.  You can also use Addgene plasmids...

  8. Deep Dive: Statistical Tests (Comparisons)

    Type
    Blog Post
    Published
    March 7, 2023, 2:15 p.m.
    ... been described in this blog. If your data does not meet the assumptions for the tests described above...to not have any effect on each other, they have discrete effects on the dependent variable, and those variables...error rate than the multiple comparison tests described above. Violated assumptions and statistics Statistical...

  9. Quick Guide to All Things Lentivirus

    Type
    Blog Post
    Published
    March 21, 2017, 2:30 p.m.
    ...inserted in this LTR so you don’t need Tat to transcribe the viral genome in producing cells. Currently...process is to test several protocols that have been described (protocol 1, protocol 2, protocol 3) to find the...transduced by a GFP encoded virus, and the reverse transcriptase activity assay uses qPCR to quantify viral RNA... RNA. All of these methods have been described in a previous Addgene blog Once you know your viral titre...

  10. Lambda Red: A Homologous Recombination-based Technique for Genetic Engineering

    Type
    Blog Post
    Published
    Dec. 15, 2016, 3:57 p.m.
    ... to screen for positive clones in most cases, and restriction enzyme digest can be used to screen plasmids...BAC DNA can be modified with any of the below described approaches.    Bacterial strain with integrated...recombination than dsDNA substrates, so you should screen more clones when using dsDNA. Have you used the...

  11. CRISPR Guide Update

    Type
    Blog Post
    Published
    Oct. 10, 2024, 1:15 p.m.
    ...update, we have added new sections to share and describe some incredible new CRISPR technologies developed...much-anticipated prime editing section and additions describing CRISPR’s ability to make large genome edits using...transposases or the Cascade-Cas3 system. We also describe newly engineered Cas9s with more flexible requirements...

  12. Plasmids 101: Modular Cloning Applications and Kits

    Type
    Blog Post
    Published
    May 14, 2024, 1:15 p.m.
    ...levels to generate anything from individual transcription units (TUs) used to express your favorite gene-of-interest...genetic circuits with multiplexed guides, mini screening libraries, direct edit optimization, and more....can be invaluable for more complex editing and screening experiments in organisms that don’t yet have these...are designed for flexibility. The Fragmid kit described above works in both mammalian systems and Drosophila...

  14. Addgene's Website Update

    Type
    Blog Post
    Published
    April 8, 2024, 2:15 p.m.
    ... The new search bar.   Pro tip! You can use a manuscript title to search for plasmids from a specific ...quickly navigate around the Addgene website without scrolling up and down the page.  Figure 3: The Education... Tools menu Further down What happens if you do scroll down? You’ll find Blugene, as well as information...

  15. Fluorescent Proteins 101: History of Fluorescent Proteins

    Type
    Blog Post
    Published
    Aug. 7, 2017, 1:58 p.m.
    ...characteristic radiance for some time – there are records describing them dating back to the first century A.D. – ...glowing protein could be used to measure gene transcription, as well as to track protein localization. And...mechanistic understanding of GFP’s function. His lab described the protein’s structure and also discovered many...

  16. Designer PUF Proteins for Any RNA Target

    Type
    Blog Post
    Published
    June 17, 2014, 7:57 p.m.
    ...ability to direct enzymes – from nucleases to transcription factors – to specific sequences of DNA has become...proteins The tool she and her colleagues led by Zhao described and demonstrated in a report published in The ...of designer PUF proteins for specific post-transcriptional regulation of endogenous RNA. J Biol Eng. 2014...

  17. Editor's Choice, September 2016

    Type
    Blog Post
    Published
    Oct. 7, 2016, 4 p.m.
    ..., P1 phage. 2) The post describes an ingenious but simple technique to screen for proteins that could ...Jessica Sacher from the University of Alberta and describes a technique you can use to find phage proteins...

  18. SciComm with the Experts at Science in the News Part 2

    Type
    Blog Post
    Published
    April 27, 2017, 2:30 p.m.
    ... discusses some of the programs SITN runs and describes some of the more popular topics from the Science...telling. Chapter 5: The SITN Blog - Vini and Amy describe how the SITN blog is a source of scientific information...underwater? Chapter 8: Work-Life Balance - Vini describes how she balances working in the lab and working...

  19. CRISPR Protocol for Genomic Deletions in Mammalian Cell Lines [Video]

    Type
    Blog Post
    Published
    Feb. 18, 2015, 3:09 p.m.
    ... paired DSBs. This method allows for deletion screening by conventional PCR. Biallelically disrupted cells...transfected cells via FACS, primer validation, and screening by PCR for biallelic deletion clones:    ...video? The Orkin and Bauer lab's related paper describes their methods for CRISPR design, cloning, and ...

  20. Tag Your Favorite Yeast Genes with Ease

    Type
    Blog Post
    Published
    Nov. 19, 2013, 2:37 p.m.
    ...These tagging vectors are based on previously described pFA6a-link vectors and include a Kan, SpHIS5, ...former lab at UNC Chapel Hill. Bähler et al.(2) describe a modular collection of plasmids for a wide variety...3xHA, 13xMyc, GST, or GFP). Longtine et al.(3) describe a complimentary set of plasmids for use in S. ...

  21. Working 9-5: an Addgene Co-op

    Type
    Blog Post
    Published
    June 2, 2022, 2:15 p.m.
    ...process When I first saw the comprehensive job description for this co-op, I was immediately interested ...had stood out to me because it gave me a full description of the tasks I would be responsible for, in comparison...and Geneious, I was able to determine if any discrepancies between what we sequenced in house and the submitted...

  22. New CRISPR Web Resources and #12DaysofCRISPR Recap

    Type
    Blog Post
    Published
    Dec. 12, 2017, 3:13 p.m.
    ...certainly taken notice! Our newest page in the Transcriptional Regulation category already has five blue flame...with eSpCas9, SpCas9-HF1, & HypaCas9 Day 10: Transcriptional regulation: Truncated gRNAs for Regulating ...Chavez) Day 11: Pooled libraries: Genome-Wide Screening Using CRISPR/Cas9 and Plasmids 101: NGS Quality...

  23. Plasmids 101: What is a plasmid?

    Type
    Blog Post
    Published
    April 2, 2020, 6:17 p.m.
    ...plasmid with its elements described below.   Vector Element Description Origin of Replication ...for further study. Promoter Region Drives transcription of the target gene. Vital component for expression...

  24. Plasmids 101: Positive and Negative Selection for Plasmid Cloning

    Type
    Blog Post
    Published
    Aug. 22, 2019, 12:43 p.m.
    ...positive selection, negative selection, and/or screening. We’re focusing on positive and negative selection...in this blog post, but don’t worry, we’ll cover screens in a future post. Positive selection: Cells that...with or without your inserted DNA) and further screening will help you determine if the plasmid backbone...

  25. Addgene-Seeding Labs Plasmid Grant: Accelerating Science Globally - 5 Scientists, 4 Countries & 3 Continents!

    Type
    Blog Post
    Published
    July 18, 2017, 12:41 p.m.
    ...activities (1). She has developed a fast endophyte screening system and plans to employ the light‐inducible...plasmids. The recombinant Cry proteins will then be screened for toxicity against local insect pests. By developing...Labs Plasmid Grant awardees' projects progress. Subscribe to the Addgene blog for updates and future collaborations...

  26. Year of the Camelid: Antibody Style

    Type
    Blog Post
    Published
    May 9, 2024, 1:15 p.m.
    ...camelids too, though not just for the reasons the UN describes. In honor of the Camelid family, I wanted to take...of times on this blog over the years and have described how they compare to other antibodies and affinity...sdAbs were used to create GFP scaffolds for transcriptional activation.  sdAbs in the clinic sdAbs have...

  27. Addgene at the ASM Microbe 2016 Conference

    Type
    Blog Post
    Published
    July 7, 2016, 2:30 p.m.
    ...well-optimized CRISPR screening technology. Addgene got several shout-outs for making popular screening libraries ...John Doench’s opening presentation at the CRISPR screens symposia outlined the fantastic work this Broad...

  28. History of CRISPR Cas - A tale of survival and evolution

    Type
    Blog Post
    Published
    Nov. 25, 2013, 10:12 p.m.
    ...CRISPR/Cas inspired genome editing was initially described by Cong et al. and Mali et al. in January 2013...Nine months later, more than 1500 publications describe work to improve the tool’s specificity, orthogonality...orthogonality, and multiplexibility in various species and describe an assortment of applications. Despite the ubiquity...

  29. Viral Vectors 101: What is a Virus (and a Viral Vector)?

    Type
    Blog Post
    Published
    Aug. 18, 2023, 4:15 p.m.
    ...retroviruses, reverse transcribe their gRNA into DNA, which is then transcribed into mRNA before being...depending on the class of virus. RNA genomes can be transcribed into mRNA, directly translated into proteins,...

  30. Viral Vectors 101: Parts of the AAV Transfer Plasmid

    Type
    Blog Post
    Published
    Aug. 11, 2020, 1:15 p.m.
    ...(WHP) Posttranscriptional Regulatory Element (WPRE) forms a tertiary structure when transcribed which ... of RNA and RNA translation, and promotes RNA transcript longevity. The polyA is located at the end of...

  31. Science Careers: Unruly Interests Feed Many Paths

    Type
    Blog Post
    Published
    Sept. 26, 2017, 12:12 p.m.
    ...programmed regulation of DNA replication and gene transcription during vertebrate embryogenesis. My interests...each day brings in new and surprisingly diverse manuscripts. I work with people, nurturing networks, as much...Career Decisions Check Out Our Career Resources Subscribe to Career Blog Posts Resources at Addgene.org...

  32. Hot Plasmids: FRET-Based Biosensors

    Type
    Blog Post
    Published
    May 6, 2014, 1:07 p.m.
    ...large-scale functional screen in bacteria. Sensor variants were then refined by a secondary screen in rat hippocampal...developed and published in 2010 for visualizing the transcription factor cAMP-responsive element-binding protein...

  33. DAP arrays for multiplex gene editing

    Type
    Blog Post
    Published
    July 7, 2022, 1:15 p.m.
    ..., lengthy genetic payloads and insufficient transcription on the array have limited the scalability and...potential in vivo applications or multiplex CRISPR screening.   Using tRNAs Luckily, a fun feature of tRNA...engineering of crops or allow for sophisticated CRISPR screenings that would perturb the unprecedented number of...

  34. Early Career Researcher Toolbox: Free Tools for Science Writing

    Type
    Blog Post
    Published
    April 28, 2021, 1:15 p.m.
    ..., it's hard to get those words out and onto the screen and transform your work into a paper or a grant... categorized either based on the section of a manuscript they might be found, i.e. introduction, methods...the Addgene blog: Tips for writing scientific manuscripts: literature searching, reading, and organizing...

  35. Virtual Science Conference Coming Up? Three Addgenies Share Their Experience

    Type
    Blog Post
    Published
    June 30, 2020, 1:15 p.m.
    ...to keep the audience engaged through a computer screen. For the Reproducibility for Everyone Workshop,...author of the poster,” he says.Viewing a poster on a screen is challenging, requiring you to zoom in to different... their poster, eliminating the need to zoom and scroll around the poster (Reshef et al., 2020).The Twitter...

  36. Luminescent Imaging with Nano-lanterns

    Type
    Blog Post
    Published
    May 25, 2017, 2:30 p.m.
    ...adoption of the technology. In 2012, Saito et al. described the first Nano-lantern, a fusion of a mutagenized...hours! The latest enhanced Nano-lanterns (eNL) described in Suzuki et al. improve the brightness of previous...substrate furimazine. In addition to the previously described applications, the enhanced signal of eNL allows...

  37. Enabling Precision Functional Genomics with the Target Accelerator Plasmid Collection

    Type
    Blog Post
    Published
    May 11, 2017, 2:30 p.m.
    ...27478040. The lung cancer mutant collection is described in this paper. Systematic Functional Interrogation...the genetic pathway reference collections are described in this paper Systematic morphological profiling...  The genetic pathway reference collection is described in this publication We also wrote an extensive...

  38. Multiplex Genome Editing with CRISPR-Cpf1

    Type
    Blog Post
    Published
    May 9, 2017, 2:12 p.m.
    ...editing!  A recent paper from Feng Zhang's lab describes how to use Cpf1 for multiplex genome editing.  ...transfection gRNAs expressed from a polycistronic transcript  Requires co-transfection of Cas9 Nissim et alTsai...transfection gRNAs expressed from a polycistronic transcript Requires co-transfection of Cas9   Xie et al  ...

  39. Top Requested Lentivirus and AAV of 2016

    Type
    Blog Post
    Published
    Jan. 6, 2017, 3:56 p.m.
    ...expressing cell lines, which can then be used both for screens (using pooled CRISPR knockout libraries) and individual...monoclonal Cas9-expression line prior to performing a screen or even a single gene knockout. Top AAV: pAAV-hSyn-DIO-hM4D... vectors and genome-wide libraries for CRISPR screening. Nat Methods. 2014 Aug;11(8):783-4. PubMed PMID...

  40. Kiran Musunuru on the Newest TALEN Genome-Editing System

    Type
    Blog Post
    Published
    Dec. 10, 2013, 3:13 p.m.
    ...Cell, Musunuru, Chad Cowan and their colleagues describe a much more efficient tool for doing that disease...modeling work in human pluripotent stem cells: a transcription activator-like effector nuclease (TALEN) kit...people unfamiliar with this new TALEN kit, can you describe it for us? Musunuru: TALENs are a type of genome...

  41. Summer SciComm Series: Cognitive Load

    Type
    Blog Post
    Published
    June 16, 2022, 1:15 p.m.
    ...formulation of new knowledge than it is to read a manuscript whose whole purpose is to provide detailed evidence...images, noises and music, spoken words, and any onscreen text. Be aware of the effort the audience is expending...Maybe I needed to employ a visual aid instead of describing something verbally. Sometimes a group was just...

  42. CRISPR Meets Synthetic Biology: A Conversation with MIT’s Christopher Voigt

    Type
    Blog Post
    Published
    April 22, 2015, 2:06 p.m.
    ...Systems Biology in November, Voigt and Alex Nielsen describe what’s possible when multi-input CRISPR/Cas genetic...many deposits to Addgene over the years. Can you describe the toolbox you are putting together?  Voigt: ... Those will allow you to set the amount of transcriptional and translational activity a cell has and control...

  43. Hot Plasmids May 2018 - Optogenetics, Decaffeination, Biosensors, and Fluorescent Protein Tools

    Type
    Blog Post
    Published
    May 15, 2018, 12:43 p.m.
    ...soCoChR-expressing system. Taken together, Shemesh et. al. describe a novel system for the precise mapping of neuronal...the single-cell level. Various soCoChR plasmids described in this work including the soCoChR-GFP plasmid...expression in E. coli. This operon is under the transcriptional control of a strong constitutive promoter (...

  44. Open Resources and Plasmid Tools For Studying C. elegans

    Type
    Blog Post
    Published
    July 18, 2019, 12:55 p.m.
    ...neurons from each class to fully characterize the transcriptome. The first data release for CeNGEN is planned... The 3’UTRs of mRNA are important for post-transcriptional regulation in C. elegans. To study the role...Addgene In addition to the wealth of open resources described above, there were many plasmid tools presented...

  45. Reaching out to China: Canton Nucleic Acids Forum (CNAF) 2015

    Type
    Blog Post
    Published
    Dec. 1, 2015, 3:30 p.m.
    ...'ve overlooked regulatory fragments of these transcripts. I remember when small RNA was just something...’t give tRNAs much thought. High throughput transcriptome sequencing methods including work done by the...circRNA datasets and download the custom python scripts needed to discover circRNAs in RNA-seq data. As...

  46. Illuminating Epigenetics with A FRET Based Biosensor

    Type
    Blog Post
    Published
    Nov. 19, 2015, 3:30 p.m.
    ...histone modifications, which can influence the transcription of genes by RNA polymerase II, or by the addition...methylcytosine in CpG islands makes  genes less transcriptionally active. The role of epigenetics in studying...of tumor suppressor genes can repress their transcription and inactivate them. Global hypomethylation,...

  47. Plasmids 101: Protein Expression

    Type
    Blog Post
    Published
    June 7, 2018, 1:17 p.m.
    ...synthesize a particular protein DNA must first be transcribed into messenger RNA (mRNA). mRNA can then be translated...template of choice and allowing these cells to transcribe, translate and modify your protein of interest...assembled in vitro using purified components of the transcription and translation machinery. These include ribosomes...

  49. Chromoproteins: Colorful Proteins For Molecular Biology Experiments

    Type
    Blog Post
    Published
    Feb. 4, 2020, 2:15 p.m.
    ...chromoproteins have a shorter history. In 1987, scientists described an unusual blue-colored protein from the jellyfish...methods. While techniques such as  blue-white screening require X-gal, an expensive exogenously-added ...chromoproteins don’t require other substrates for screening. Thus chromoproteins can be used as markers in...

  50. AAV Vector Quality Control: Going the Extra Mile with NGS

    Type
    Blog Post
    Published
    Sept. 12, 2017, 1:44 p.m.
    ...transduced cells whenever possible. This post will describe our workflow for viral genome sequencing (VGS)...identification, we developed custom open-access Python scripts that can perform a deeper analysis of the data....Check out our Viral Vectors Featured Topic Page Subscribe to Viral Vectors Blog Posts Learn How We Use NGS...
Showing: 201 - 250 of 612 results