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TypeBlog PostPublishedJuly 16, 2015, 2:30 p.m....J. Hecksher-Sorensen, R. Baldock, D. Davidson. (2002) Science, 296, 541-545. Pubmed. A. Ertürk, C.P. ...
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CRISPR 101: Ribonucleoprotein (RNP) Delivery
TypeBlog PostPublishedSept. 6, 2018, 12:02 p.m.... PubMed PMID: 29668265. PubMed Central PMCID: PMC6002863. 4. Liang, Zhen, et al. "Efficient DNA-free genome... -
Negative Can Be Positive: Open AAV Data with Addgene
TypeBlog PostPublishedSept. 6, 2022, 1:15 p.m....(5), 392–400. https://doi.org/10.1038/s41434-018-0028-5 de Backer, M. W. A., Brans, M. A. D., Luijendijk... -
An Introduction to Adenovirus
TypeBlog PostPublishedOct. 3, 2023, 1:15 p.m...., 526–534. https:/doi.org/10.1016/S1525-0016(03)00023-6. PMID: 12727116. Borkenhagen, L. K., Fieldhouse... -
Viral Vectors 101: The Retroviral Lifecycle
TypeBlog PostPublishedJuly 27, 2023, 1:15 p.m....Biology, 79(1), 163–183. https://doi.org/10.1016/0022-2836(73)90277-5. PubMed PMID: 4355596 King, S. R... -
Fluorescent Proteins 101: Aptamer Fluorophores
TypeBlog PostPublishedApril 11, 2017, 2:30 p.m....cells.” Proc. Natl. Acad. Sci. USA 112(10) (2015): 3002-7. PubMed PMID: 25713381. PubMed Central PMCID: ... -
Advancing Biology with Zebrafish: Genetic Tools for Developmental Studies and More
TypeBlog PostPublishedAug. 13, 2019, 12:58 p.m....Science 360.6392 (2018): eaar3131. PubMed PMID: 29700225. PubMed Central PMCID: PMC6247916. Felker, A.,... -
Twenty Years of Sharing: Addgene's Viral Vector Service
TypeBlog PostPublishedJuly 30, 2024, 1:15 p.m.... Biology, 115, 31.5.1-31.5.13. https://doi.org/10.1002/cpmb.8... -
Overcoming the AAV Size Limitation for CRISPR Delivery
TypeBlog PostPublishedSept. 16, 2020, 1 p.m....Biotechnology Journal 9:1402–1412 . https://doi.org/10.1002/biot.201400046 Strecker J, Jones S, Koopal B, ... -
Hot Plasmids - November 2023
TypeBlog PostPublishedNov. 14, 2023, 3:35 a.m....compact, efficient prime editors. Cell, 186(18), 3983–4002.e26. doi: 10.1016/j.cell.2023.07.039. PMID: 37657419... -
Viral Vectors 101: Preparing Pooled Libraries
TypeBlog PostPublishedJuly 13, 2023, 1:15 p.m.... Mol Biol. 2016 Jul 1;115:31.5.1-31.5.13. doi: 10.1002/cpmb.8. PMID: 27366891. Joung J, Konermann S, Gootenberg... -
QC Sequencing Technologies at Addgene
TypeBlog PostPublishedJuly 15, 2025, 1:15 p.m.... in Molecular Biology, 122(1). https://doi.org/10.1002/cpmb.59 Wang, Y., Zhao, Y., Bollas, A., Wang, ... -
The Challenges of Cell Culture
TypeBlog PostPublishedMay 5, 2016, 2:30 p.m....the bad and the ugly."Nature Reviews Cancer 2.4 (2002): 315-319. PubMed PMID: 12001993. 4. Lucey, Brendan... -
Getting the Most from Your Lentiviral Transduction
TypeBlog PostPublishedApril 7, 2016, 2:30 p.m...., elimination, prevention.” Cytotechnology 39.2 (2002). 75-90. Pubmed PMID: 19003295. 4. Uphoff, C.C.,... -
Tips for Using FRET in Your Experiments
TypeBlog PostPublishedNov. 5, 2014, 3:41 p.m....cells. Bioessays. 2012 May; 34(5):341-50. doi: 10.1002/bies.201100098. Sekar RB, Periasamy A. Fluorescence... -
Five Popular Model Organisms, Part 2
TypeBlog PostPublishedSept. 29, 2020, 1:15 p.m....Mechanisms, 2(5–6), 206–210. https://doi.org/10.1242/dmm.002733 Idalia, V.-M. N., & Bernardo, F. (2017). Escherichia... -
Antibodies 101: Single Chain Fragment Variables (scFvs)
TypeBlog PostPublishedJune 3, 2021, 1:15 p.m....Fragments. Antibodies 8:28 . https://doi.org/10.3390/antib8020028 Bird R, Hardman K, Jacobson J, Johnson S, Kaufman... -
CRISPR 101: Engineering the Plant Genome Using CRISPR/Cas9
TypeBlog PostPublishedOct. 11, 2016, 2:30 p.m....21:661–677 . https://doi.org/10.1038/s41580-020-00288-9 Additional Resources on the Addgene Blog Read... -
Using Phosphoserine to Study Protein Phosphorylation
TypeBlog PostPublishedJune 23, 2016, 2:30 p.m....protein phosphorylation." Nature cell biology4.5 (2002): E127-E130. PubMed PMID: 11988757. 2. Hornbeck,... -
Plasmids 101: Repressible Promoters
TypeBlog PostPublishedDec. 20, 2022, 2:15 p.m....Biotechnol. 2019 Jan;103(1):303-313. doi: 10.1007/s00253-018-9485-4. Epub 2018 Nov 3. PMID: 30392122. Pfeiffer...