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Showing: 81 - 100 of 270 results

  1. PCR Applications for Copy Number Variation Assays

    Type
    Blog Post
    Published
    April 26, 2022, 1:15 p.m.
    ...calculations so that your assay output number (RQ value) matches the copy number of your sample. In that case, an...options, you’re likely to find an application that matches your needs. Happy quantifying!  References and...

  2. Drew Endy Introduces the Biobrick Public Agreement Plasmid Collection

    Type
    Blog Post
    Published
    Dec. 12, 2013, 3:35 p.m.
    ...everybody. (For more on these tools and how they work, watch Endy's 10-minute YouTube primer on BIL gates.) Addgene...systems today who are building 10-gene systems from scratch. (They may be building genomes, but that's just...

  3. Antibiotic Resistance: An Old Solution but a New Problem

    Type
    Blog Post
    Published
    Nov. 10, 2016, 3:30 p.m.
    ...following the tried and true process of evolution. Watch this video showing bacteria evolve antibiotic resistance...antibiotic resistance. Source: https://www.youtube.com/watch?v=sx1uDYSfINA. When you go to the doctor, it will...

  4. 5 Reasons to Use Reddit for Science Communication

    Type
    Blog Post
    Published
    Oct. 20, 2016, 2:30 p.m.
    ...Reddit Science is heavily moderated. I started watching the questions and comments go up early in the ...mechanics of doing the AMA were interesting. I started watching the questions and preparing answers early in the...

  5. Analyzing CRISPR Editing Results with ICE from Synthego

    Type
    Blog Post
    Published
    May 8, 2018, 1 p.m.
    ... sequencing files, either one at a time or as a batch of hundreds, and indicate the guide RNA sequence...compare up to five editing experiments at a time, and batch analysis, which compares hundreds of samples simultaneously...

  6. Designing Your Chalk Talk for the Academic Job Interview

    Type
    Blog Post
    Published
    March 12, 2019, 1:37 p.m.
    ...vision with them. Do not noticeably look at your watch. Be enthusiastic. This is your research program....Addgene.org Learn more about careers at Addgene Watch our career videos ...

  8. Viral Production at Addgene

    Type
    Blog Post
    Published
    Oct. 24, 2023, 1:15 p.m.
    ...transfer plasmid Addgene uses a rigorous barcode matching system that follows the sample from the DNA tube...    Figure 4: Schematic of the barcode matching system and PCR-based confirmation of transfer ...

  9. Tips for Writing a Good Cover Letter

    Type
    Blog Post
    Published
    May 21, 2024, 1:30 p.m.
    ...for you to explain how your skills and experience match the job requirements, by giving specific examples... a good candidate, explain how your experience matches the job requirements, and address any unusual circumstances...

  10. CRISPR 101: Which Cas9 Do I Choose for My CRISPR Experiment?

    Type
    Blog Post
    Published
    Oct. 13, 2022, 1:45 p.m.
    ...repository) and make it easy to find your perfect match. Choosing a Cas9 for your knockout experiment Generating...resources on the Addgene website Find CRISPR Plasmids Catch up on your CRISPR background with our CRISPR Guide...

  11. CRISPR 101: Targeting RNA with Cas13a (C2c2)

    Type
    Blog Post
    Published
    Sept. 21, 2017, 2:07 p.m.
    ... molecule. Like Cas9, Cas13a tolerates single mismatches between the crRNA and target sequence, however...cutting efficiency of Cas13a is reduced when 2 mismatches are present. The protospacer flanking sequence...

  12. Important Considerations in Optogenetics Behavioral Experiments

    Type
    Blog Post
    Published
    Oct. 1, 2015, 2:30 p.m.
    ...after all the optimization, turn on the laser and watch what happens. Who knows, you may observe something...Technical differences include the ferrule implants and patch cables. One huge advantage of using rats is their...

  13. Antibodies 101: Introduction to Immunofluorescence

    Type
    Blog Post
    Published
    Nov. 16, 2021, 2:15 p.m.
    ...trusted secondary antibodies on hand to mix and match with a larger collection of primary antibodies. ...suits your experiment (i.e. the spectral properties match your available imaging instruments and the spectral...

  14. Hot Biosensors 2022: Year-End Roundup

    Type
    Blog Post
    Published
    Dec. 6, 2022, 2:15 p.m.
    ...biosensor with a binding affinity that is better-matched to the potassium concentrations in cells than existing...lactate biosensors, and its affinity is better-matched to cellular concentrations of lactate. The microfluidic...

  15. A Control for All Seasons

    Type
    Blog Post
    Published
    Oct. 5, 2023, 1:15 p.m.
    ...animal the antibody was produced in or an isotype-matched control antibody. This control addresses situations...in preimmune serum ‌as well as with an isotype-matched control.      Figure 3: A preimmune serum...

  16. CRISPR 101: Cytosine Transversion Editors

    Type
    Blog Post
    Published
    March 25, 2025, 1:15 p.m.
    ...excision repair (BER), the cellular process that catches mutated U’s in DNA and ideally converts them back...gRNA/sgRNA Guide RNA RNA used by Cas9 to locate a matching DNA sequence.   Target strand The DNA strand...

  17. Technical Design of a Western Blot

    Type
    Blog Post
    Published
    Aug. 22, 2024, 1:15 p.m.
    ...produced from plasmids and have very little batch-to-batch variation. Confirmation of sequence can be ...

  18. Troubleshooting and Optimizing a Western Blot

    Type
    Blog Post
    Published
    Sept. 17, 2024, 1:15 p.m.
    ...even at 4 °C, so when in doubt, make up a fresh batch.  Antibodies We always recommend either using an...sure the sensitivity of your exposure solution matches the expected amount of protein in your samples....

  19. 22 Hot Plasmid Technologies from 2014

    Type
    Blog Post
    Published
    Jan. 6, 2015, 5:21 p.m.
    ....addgene.org/CRISPR/ to browse CRISPR plasmids, watch informational videos, download protocols, and more...ligation using SpyLigase Building off their SpyTag/SpyCatcher system for protein-peptide locking, Mark Howarth...

  20. New Antibodies in the Lab Animation!

    Type
    Blog Post
    Published
    Nov. 19, 2024, 2:15 p.m.
    ...these common antibody applications.  Thanks for watching, and we hope you found the video radd-ically helpful...
Showing: 81 - 100 of 270 results