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We narrowed to 277 results for: ATC

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Showing: 81 - 100 of 277 results

  1. Addgene's Tips for Plasmid Quality Control

    Type
    Blog Post
    Published
    Jan. 14, 2016, 3:30 p.m.
    ... important step in ensuring quality, as it will catch any errors made during the cloning, sample handling... sequences to visually check for homology and mismatches. Do you have your own best sequencing practices...

  2. Quickest Way to Deposit Plasmids: The Deposit Spreadsheet

    Type
    Blog Post
    Published
    March 8, 2017, 3:15 p.m.
    ...Plasmid Name Use a descriptive plasmid name and match the name as it appears in its publication or as ...spreadsheet, save it in the format: labname_addgene_batch_upload.xls Replace ‘labname’ with the name of...

  3. Tips for Titering Your Lentiviral Preps

    Type
    Blog Post
    Published
    March 15, 2016, 2:30 p.m.
    ...standard conditions be used when comparing different batches of virus. In addition, some viral preps are very... stored in the freezer. If you plan to freeze a batch of virus for long term use, you may want to consider...

  4. 10 Basic tips for mammalian cell culture

    Type
    Blog Post
    Published
    July 12, 2018, 1:09 p.m.
    ...additionally remove all jewellery as well as that watch you’re wearing before you start. Also, avoid unnecessary...cell culture contamination in this post from the ATCC Declutter! Keep the hood clean Hoods should not ...

  5. PCR Applications for Copy Number Variation Assays

    Type
    Blog Post
    Published
    April 26, 2022, 1:15 p.m.
    ...calculations so that your assay output number (RQ value) matches the copy number of your sample. In that case, an...options, you’re likely to find an application that matches your needs. Happy quantifying!  References and...

  6. AAV Vector Quality Control: Going the Extra Mile with NGS

    Type
    Blog Post
    Published
    Sept. 12, 2017, 1:44 p.m.
    ...very low concentration. So how do we make sure to catch all of these potential mishaps before distributing...suspect is contaminated. For AAV-68544, the best match for the contaminant is Addgene plasmid #44362. Method...

  7. Drew Endy Introduces the Biobrick Public Agreement Plasmid Collection

    Type
    Blog Post
    Published
    Dec. 12, 2013, 3:35 p.m.
    ...everybody. (For more on these tools and how they work, watch Endy's 10-minute YouTube primer on BIL gates.) Addgene...systems today who are building 10-gene systems from scratch. (They may be building genomes, but that's just...

  8. Analyzing CRISPR Editing Results with ICE from Synthego

    Type
    Blog Post
    Published
    May 8, 2018, 1 p.m.
    ... sequencing files, either one at a time or as a batch of hundreds, and indicate the guide RNA sequence...compare up to five editing experiments at a time, and batch analysis, which compares hundreds of samples simultaneously...

  9. CRISPR 101: Which Cas9 Do I Choose for My CRISPR Experiment?

    Type
    Blog Post
    Published
    Oct. 13, 2022, 1:45 p.m.
    ...repository) and make it easy to find your perfect match. Choosing a Cas9 for your knockout experiment Generating...resources on the Addgene website Find CRISPR Plasmids Catch up on your CRISPR background with our CRISPR Guide...

  10. CRISPR 101: Targeting RNA with Cas13a (C2c2)

    Type
    Blog Post
    Published
    Sept. 21, 2017, 2:07 p.m.
    ... molecule. Like Cas9, Cas13a tolerates single mismatches between the crRNA and target sequence, however...cutting efficiency of Cas13a is reduced when 2 mismatches are present. The protospacer flanking sequence...

  11. Designing Your Chalk Talk for the Academic Job Interview

    Type
    Blog Post
    Published
    March 12, 2019, 1:37 p.m.
    ...vision with them. Do not noticeably look at your watch. Be enthusiastic. This is your research program....Addgene.org Learn more about careers at Addgene Watch our career videos ...

  12. Viral Production at Addgene

    Type
    Blog Post
    Published
    Oct. 24, 2023, 1:15 p.m.
    ...transfer plasmid Addgene uses a rigorous barcode matching system that follows the sample from the DNA tube...    Figure 4: Schematic of the barcode matching system and PCR-based confirmation of transfer ...

  13. Tips for Writing a Good Cover Letter

    Type
    Blog Post
    Published
    May 21, 2024, 1:30 p.m.
    ...for you to explain how your skills and experience match the job requirements, by giving specific examples... a good candidate, explain how your experience matches the job requirements, and address any unusual circumstances...

  15. Antibiotic Resistance: An Old Solution but a New Problem

    Type
    Blog Post
    Published
    Nov. 10, 2016, 3:30 p.m.
    ...following the tried and true process of evolution. Watch this video showing bacteria evolve antibiotic resistance...antibiotic resistance. Source: https://www.youtube.com/watch?v=sx1uDYSfINA. When you go to the doctor, it will...

  16. 5 Reasons to Use Reddit for Science Communication

    Type
    Blog Post
    Published
    Oct. 20, 2016, 2:30 p.m.
    ...Reddit Science is heavily moderated. I started watching the questions and comments go up early in the ...mechanics of doing the AMA were interesting. I started watching the questions and preparing answers early in the...

  17. Important Considerations in Optogenetics Behavioral Experiments

    Type
    Blog Post
    Published
    Oct. 1, 2015, 2:30 p.m.
    ...after all the optimization, turn on the laser and watch what happens. Who knows, you may observe something...Technical differences include the ferrule implants and patch cables. One huge advantage of using rats is their...

  18. Hot Biosensors 2022: Year-End Roundup

    Type
    Blog Post
    Published
    Dec. 6, 2022, 2:15 p.m.
    ...biosensor with a binding affinity that is better-matched to the potassium concentrations in cells than existing...lactate biosensors, and its affinity is better-matched to cellular concentrations of lactate. The microfluidic...

  19. Antibodies 101: Introduction to Immunofluorescence

    Type
    Blog Post
    Published
    Nov. 16, 2021, 2:15 p.m.
    ...trusted secondary antibodies on hand to mix and match with a larger collection of primary antibodies. ...suits your experiment (i.e. the spectral properties match your available imaging instruments and the spectral...

  20. A Control for All Seasons

    Type
    Blog Post
    Published
    Oct. 5, 2023, 1:15 p.m.
    ...animal the antibody was produced in or an isotype-matched control antibody. This control addresses situations...in preimmune serum ‌as well as with an isotype-matched control.      Figure 3: A preimmune serum...
Showing: 81 - 100 of 277 results