We narrowed to 148 results for: Coli

1. Inside Addgene's 10 Year Anniversary Gala

   Type

   Blog Post

   Published

   July 3, 2014, 3:48 p.m.

   ...to make copies of the newspaper, Addgene uses E. coli bacteria to generate copies of plasmids. The community...

2. 22 Hot Plasmid Technologies from 2014

   Type

   Blog Post

   Published

   Jan. 6, 2015, 5:21 p.m.

   ...phosphorylated amino acid to the genetic code of E. coli (Park et al., Science 2011). The Rinehart lab has...methodology in two differents types of bacteria (E. coli and Salmonella typhimurium) by integrating DNA sequences...expression and purification of Cas9 protein in Rosetta E. coli. New Lentiviral CRISPR activator and repressor plasmids...constructs constrained only by the ability of E. coli to maintain the final plasmid after transformation...

3. Plasmids 101: Positive and Negative Selection for Plasmid Cloning

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   Blog Post

   Published

   Aug. 22, 2019, 12:43 p.m.

   ...synthesizes the critical amino acid). Many common E. coli strains used for cloning are auxotrophic for specific...

4. A New Optogenetic Tool Based on AraC Controls Gene Expression with Blue Light

   Type

   Blog Post

   Published

   June 17, 2021, 1:17 p.m.

   ...called BLADE (blue light-inducible AraC dimers in E. coli) that turns expression on and off with blue light...

5. Bright Monomeric Fluorescent Proteins: mNeonGreen, mTFP1, and mWasabi

   Type

   Blog Post

   Published

   April 25, 2019, 3:01 p.m.

   ...University of Oregon created mTFP1 by transforming E. coli with a gene library of approximately 484 variants...

6. 10 Great Guest Posts We're Thankful For

   Type

   Blog Post

   Published

   Nov. 18, 2016, 3 p.m.

   ...proteins. She also showed that R-bodies can rupture E. coli membranes. Read the full post for potential applications...

7. Walking beside Academia and giving it a high five: My transition from graduate work to a position at Addgene

   Type

   Blog Post

   Published

   July 2, 2015, 8:37 p.m.

   ... my days (and often my nights :D) engineering E. coli to produce biofuels in Pamela Silver’s Lab (plasmid...

8. When Fidelity Matters: A frank discussion about ligase fidelity

   Type

   Blog Post

   Published

   March 6, 2018, 1:49 p.m.

   ...thermophilus DNA ligase, a homolog of Escherichia coli DNA ligase." Journal of bacteriology 173.16 (1991...Last stop on the road to repair: structure of E. coli DNA ligase bound to nicked DNA-adenylate." Molecular...

9. How to Deposit Your Plasmids with Addgene

   Type

   Blog Post

   Published

   Feb. 27, 2024, 2:15 p.m.

   ...distribute your bacterial strains if they: are E. coli that can be used under BSL1 conditions were generated...you to produce large amounts of your insert in E. coli, then this is a Bacterial Expression vector. If ...

10. Plasmids 101: NGS Quality Control for Pooled Libraries

    Type

    Blog Post

    Published

    Oct. 26, 2017, 1:59 p.m.

    ... Library amplification seems straightforward: E. coli bacteria are transformed with the library DNA, they...

11. History of CRISPR Cas - A tale of survival and evolution

    Type

    Blog Post

    Published

    Nov. 25, 2013, 10:12 p.m.

    ...CRISPR sequences were initially discovered in E. coli in 1987 (1), the concept that these clustered repeat...

12. Plasmids 101: Inducible Promoters

    Type

    Blog Post

    Published

    Jan. 18, 2018, 2:34 p.m.

    ...Cre-lox Light-Switchable Two-Component Systems for E. coli Browse other blog posts from our Plasmids 101 series...

13. A Novice's Perspective on Neuroscience - SfN2017

    Type

    Blog Post

    Published

    Dec. 15, 2017, 2:07 p.m.

    ...you're currently a synthetic biologist engineering E. coli metabolism, maybe you’ll be able to apply your knowledge...

14. A Deep Dive into BioBuilder

    Type

    Blog Post

    Published

    Oct. 11, 2017, 1:44 p.m.

    ...Standards Synthetic Photobiology: Optogentics for E. coli Browse All Synthetic Biology Blog Posts Resources...

15. Hot Plasmids - March 2020 - base editors, GEVI, MoClo, and optogenetics

    Type

    Blog Post

    Published

    March 3, 2020, 2:15 p.m.

    ...construct plasmids for use in model organisms such as E. coli and yeast. Because this approach uses standardized...

16. Hot Plasmids - February 2022

    Type

    Blog Post

    Published

    Feb. 22, 2022, 2:15 p.m.

    ...20, 30, 40, 50, 60, 80 and 100 kD proteins in E. coli, either individually (~20,000 lanes/50 mLs) or through...

17. Troubleshooting Your Plasmid Cloning Experiment

    Type

    Blog Post

    Published

    Sept. 24, 2019, 12:55 p.m.

    ...not work. Check the plates! Streak on them an E. coli  strain known to be sensitive to that antibiotic...

18. Hot Plasmids - June 2019 - Optogenetics, Acoustic Reporter Genes, microRNAs, and the CRISPR-Cas9 system CHIME

    Type

    Blog Post

    Published

    June 6, 2019, 12:41 p.m.

    ...visualized in vivo using ultrasound in Escherichia coli and Salmonella Typhimurium. This work demonstrates...

19. Hot Plasmids December 2018 - C. elegans RNAi, TurboID, Detecting Viral Outbreaks, and Protein Binding Specificity Tools

    Type

    Blog Post

    Published

    Dec. 6, 2018, 1:34 p.m.

    ...function. RNAi is commonly achieved by feeding E. coli expressing dsRNA to C. elegans. These dsRNAs target...

20. Plasmids 101: Sequence and Ligation Independent Cloning (SLIC)

    Type

    Blog Post

    Published

    Sept. 26, 2024, 1:15 p.m.

    ...robust system of homologous recombination found in E. coli, SLIC is a cheap, standardized, and rapid multi-...