We narrowed to 559 results for: ESP

...sequence, a part of the env gene containing the rev response element (RRE), an internal promoter, and the desired...generation of replication competent lentiviruses (RCL) especially if you are working on HIV positive human cell...generating RCL is low but extra care has to be taken especially if you are working with proto-oncogenes or with...the Tet-on system. In the Tet-off system a Tet-response element (TRE) is placed upstream of the promoter...

...life-saving research are being proposed.  It's our responsibility to join the public conversation and to tell...was supremely proud of Nashville that morning. Despite the rain, about 4,000 supporters of science showed...towards starting these conversations; it is our responsibility to continue the dialogue. Erin Golden is currently...continue this work by voting for candidates that respect science, learning to communicate their science ...

...cancer are often time consuming and expensive, especially when done in vivo. There are also sensitivity...Atlas (TCGA), a catalog of genetic mutations responsible for cancer from genomics data, and large-scale...mutation or gene fusion. Plasmids carrying the corresponding wild-type genes were constructed as well. They...

...interest and curiosity to understand my work and me, especially as much of her focus is on the scientific work...been done at Addgene before. This meant I was responsible for establishing a new report and routine across..., and make the occasional TikTok. I was also responsible for typical entry level tasks such as stuffing...

...factor in maintaining the group buy-in and morale. Especially when your collaboration includes scientists working...a time. Just be sure there is a point person responsible for incorporating everyone’s suggestions into...timelines for experiments and manuscripts, who is responsible for which parts of the project, and, as mentioned...

...single-wavelength sensors with intensities that change in response to calcium binding. There are a few versions of...endoplasmic reticulum, but exhibits larger fluorescent responses to GABA. Find the iGABASnFR and its F102G variant...with optogenetic activators and inhibitors that respond to blue light. We recently released a constitutive...

...that can extend and retract over many microns in response to pH changes without the need for ATP or other...In her work, Dr. Polka made R-body mutants that respond to different pH’s and some that are fused to fluorescent... neuronal pathways using light and chemicals respectively. Didem Goz Aytruk from Connie Cepko’s Lab at...

... career in neuroscience—whatever that entails. Despite being engrossed by the theory and findings of brain...instrument designed to measure how individuals respond to issues of unsettled science. Understanding just...to understand just how we receive, process and respond to that information. Alex Gilbert is a PhD student...

...major advantage of working with a mentor.The responsibility of arranging meetings and setting the direction...informal minutes on what was covered and learned, especially noting action items that are identified. Keep...checks. Focus on implementation of your goals and respect the time your Mentor is giving by following up ...

... council – all worked in tandem, within their respective mandates, to tackle a controversial and ethically...” or whether the media and academic conference response to He created a positive feedback loop that rewarded..., and they are not remembered kindly. However, despite many headlines questioning if He’s experiments ...

...Regulating translation is key to cellular function, especially during development or stress. With ribosome profiling...devised a system that labels both mRNAs and their corresponding newly synthesized proteins. As in the TRICK ... usually translated in a specific compartment. Despite these limitations, these techniques represent powerful...

...about the people that don’t respond; focus on the nice people who do respond. Don’t call or email multiple...multiple times if you don’t get a response. It breaks an important law of networking--don’t be creepy. There...

...what will be packaged. The 5’ LTR and 3’ LTR, respectively, act as RNA polymerase II promoter and terminator...packaging of the virus into particles. E2A/E2B are responsible for viral replication, with E2B being the primary... suppresses both apoptosis and the host immune response to the virus to allow it to evade detection by...

...research. These fluorescent proteins light up in response to calcium, which is more than just the stuff ...mouse cortex. Bottom: Zoomed-in view of traces corresponding to the dashed box above. The number of action...indicates a single AP. B) Averaged fluorescence responses to increasing numbers of evoked APs in neurons...

...preparation and a willingness to embrace new responsibilities. After my second postdoc, I realized that... team and allow myself to focus on my core responsibilities, which typically revolved around the broader...started at my previous company by taking on responsibilities such as mentoring a junior colleague. Guiding...

... available collection of mutant cDNA clones corresponding to cancer variants found in genome sequencing...include 634 sequence confirmed mutant cDNA clones corresponding to cancer variants derived from analysis of ...aggregate a sequence-confirmed series of clones corresponding to known cellular signaling pathways as part...

...correlating them with tumor mutations and clinical responses. This platform has the potential to become an ... to control immune signaling and inflammatory responses remotely with spatiotemporal precision. This is...function of immune cells that are not excitable but responsive to calcium. We’d love to expand our cancer and...

...vaccines, including 1) cellular responses in addition to antibody response 2) very high immunogenicity 3... 3) and long-lasting immune response even after a single dose. Vaccine development strategies. Image...

...everything. If it’s barcoded, it should have a corresponding entry in your database. Enjoy your happy lab...at some point, decide early on what to do in a namespace collision. It may even be worth it to have extra... you're solving, but consistency is key. Some namespace collisions you may encounter: vector, sequence...

...always a good idea to perform primer optimization, especially if you are having difficulty amplifying your ...colonies, check that the antibiotic in the plate correspond to the antibiotic resistance marker present in...small culture volume (2 or 3 ml) containing the corresponding antibiotic, extract the plasmid, and analyze...