...the exciting new field we call “precision functional genomics.” The goal of this field is to functionally...correlations between variants in a gene of poorly classified function and a well established cellular signaling...Functional Interrogation of Rare Cancer Variants Identifies Oncogenic Alleles. Kim E, Ilic N, Shrestha Y....

...virus, introduced to the field of fusion proteins more than thirty years ago (Field et al., 1988). Thus, ...experiments. Fluorescent proteins can also be identified using antibodies and immunohistochemistry techniques...–5168 . https://doi.org/10.1073/pnas.0700293104 Field J, Nikawa J, Broek D, MacDonald B, Rodgers L, Wilson...

...Scientific Communication Preprints have been common in fields like math and physics for decades (see: arXiv.org...commented on by readers, typically peers in the field, and updated by the authors over time, providing...subscriptions to the specialized journals in your field? Post a preprint before you submit to a journal ...accessible, allowing problematic work to be quickly identified and rigorously discussed and addressed by the...

...In vitro tests for aggregation The tendency of purified fluorescent proteins to form homodimers or higher...what extent does homodimerization assayed with purified protein, reflect the likelihood that fluorescent...OSER) whorls are visible. These whorls can be quantified for individual cells, and the number of 'normal...under a limited number of conditions, often using purified proteins. Therefore, it is essential to verify...

... intense light (for a brief time), whereas in widefield imaging fluorophores are excited with relatively...Photostability measurements can be performed on purified fluorescent proteins. To avoid diffusion, the ...the most photostable fluorescent protein can be identified by a head-to-head comparison of several fluorescent...comparison between cyan fluorescent proteins using widefield imaging. Our choice for a photostable protein ...

...these limitations and lead to new milestones in the field. Research strategy The Research Strategy highlights...research challenge. Demonstrating expertise in the field and past successes boost chances in receiving funding... address a variety of challenges and impact the field - its encouraged to dream big! It is crucial that...into the mission of the agency and benefits the field reminds the reviewer why your project is the project...

...individual bases can be modified. Cytidine deaminase PmCDA1 Akihiko Kondo’s lab identified a cytidine deaminase...Through recombineering, the locus of interest is modified to match the repair template, and the locus cannot...more efficient, well tolerated, Cas enzyme. They identified the Cas9 orthologue from Streptococcus thermophilus...Sun B, Chen J, Zhang Y, Jiang Y, Yang S (2021) A modified pCas/pTargetF system for CRISPR-Cas9-assisted ...

... Gaudelli et al., 2020. Directed evolution identifies adenine base editors with improved editing in...described TadA mutations (Gaudelli et al., 2017), they modified ABE7.10 accordingly which resulted in variants...the “x” is the placeholder for the number that identifies the mutation in the evolved TadA domain. Because...cells using 8 selected ABE8s and ABE7.10. They quantified genomic editing by measuring the reduction in...

...2019.       Direct ELISA In a direct ELISA, purified proteins or the experimental sample are bound ...instead of an antibody. Coating the wells with a purified protein allows you to characterize properties ...antibody, a primary-secondary pair is used. This amplifies the signal as multiple secondary antibodies can... if that’s the option you choose. Once you’ve identified the linear portion of your standard curve, you...

...those who are not experts in particular scientific fields. As scientists, many of us are used to being very...focused on narrowly-defined research questions in our field of interest. We often communicate in very technical...presentations. But for many people who don’t work in these fields of research, what often concerns them the most ...interest and awareness of issues outside of your field of research will allow you to better engage these...

...and Alexander A. Green. Zika background First identified in 1947 in Uganda, the Zika virus had received...developed two new enabling technologies for the field of synthetic biology. The first was a new class ...advancing the paper-based platform toward a more field-ready format when the Zika virus outbreak was reported...felt that, given the critical need for low-cost, field-based diagnostics, our approach could make an impact...

...professional organization or a scientific society in your field. My leadership training began with the NPA where... within a professional society in my scientific field - ARVO (as mentioned above). I became an official...closer connection with other leaders in my research field should I decide to remain here.  In a time when ... skills can only assist you and make you more qualified for future career opportunities. Becoming involved...

...during the extraction procedure. Additionally, purified DNA was free of agarose and could be digested ...contaminants while nucleic acids stick around. Finally, purified total nucleic acids are released by dipping the...directly into a PCR buffer, where DNA or RNA can be amplified. Compared to a commercial magnetic bead-based ...material: PCR product or agarose gel slices Product: purified PCR product or DNA fragments Maybe you’re not ...

...collaboratively discussing recent publications in a specific field(s). Most anyone who has spent any time in academia...the literature, sampling a breadth of work from a field (perhaps even two articles per meeting) would be...people feel confident in their own knowledge of the field. Perhaps interest has simply waned. When attendance... a way to learn about work outside of one's own field in a structured and interactive way. Like any meeting...

...interactions. The 1980s saw a flurry of discovery in the field of eukaryotic transcriptional activation and cell... And most importantly: Independently validate identified binding partners with other techniques! Minimizing...that your proteins are not post-translationally modified, co-expression of the enzyme responsible for the...Cell. 1985; 43:729-736. PubMed PMID: 3907859. 4. Fields, S., Song, O. A novel genetic system to detect ...

... testing its function. Small ubiquitin-related modifier (SUMO) can help with folding and stabilization...to affect function, His-tagged proteins can be purified using metal-affinity chromatography, usually using...protease around, but it is convenient when used with purified protein since the enzyme cleaves the SUMO tag ...increase its stability activity, can be readily purified using plasmids found in this paper (available ...

...searched for this unique sequence of DNA and both identified lymphocyte antigen 6 complex (LY6A) as a receptor...hematopoietic stem cells. Its ligand(s) has not been identified. While LY6A or other Ly6 gene family members ...susceptibility. The Deverman and Wilson Labs independently identified LY6A as a receptor for PHP.B by looking for a...total, they tested 13 strains, with seven strains identified as permissive and six as non-permissive to PHP.B...

... ICE software identifies the percentage of genomes that have been successfully modified with insertions...engineering has revolutionized the gene editing field by making experimental workflows considerably easier...edited and unedited (control) populations is PCR-amplified and Sanger sequenced. ICE compares these sequence...

...through the expression of a shRNA against p53, a modified reprogramming factor cocktail, and viral protein...trials and remains a safety concern in the clinical field [4]. Three major reprogramming methods are considered...General Hospital with research interests in the fields of gene editing and stem cells. Connect with her...Kotton Four Factors that Differentiate the Stem Cell Field Sign Up to Write for the Addgene Blog! Resources...

..., targeted genome modifications. Cas9 has been modified to enable researchers to knock out, knock in, ...Prime editors are the second generation of base modifiers available. This system utilizes an RNA template...activator, consists of a dCas9-VP64 fusion and a modified gRNA that is capable of interacting with an additional... to confirm that your target sequence has been modified!  The exact protocol may vary depending on your...