We narrowed to 354 results for: HRE
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TypeBlog PostPublishedAug. 3, 2023, 1:15 p.m....needs to be used with spectral flow—you can run a three color panel or a 40 color panel! When preparing ...Similarity index across 12 different colors on a three laser spectral flow cytometer. Level of co-expression...
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Of Myc and Men
TypeBlog PostPublishedJan. 19, 2023, 2:15 p.m....antibodies (Evan, et al. 1985). Three bound to the mid-protein peptide, while three bound to the C-terminal peptide... -
Quick Guide to Working with Drosophila Part 1: Getting Started with Flies
TypeBlog PostPublishedJuly 13, 2017, 2:30 p.m.... a living organism. This is the first post in a three-part series. We’ll first discuss how to get started... into embryos and then become larvae. There are three larval stages where larvae molt in order to continue... -
Starter guide to induced pluripotent stem cells (iPSCs) part 1: A renaissance in regenerative medicine
TypeBlog PostPublishedOct. 30, 2018, 1:04 p.m....ability to differentiate into cells of any of the three germ layers: endoderm, ectoderm, and mesoderm. In... differentiate in vitro into derivatives of all three germ layers, form embryoid bodies, and form teratomas... -
PhD Applications After COVID
TypeBlog PostPublishedJan. 5, 2023, 2:15 p.m....await a reply. It’s a good idea to email around three professors per program. Most applications will recommend...application that you intend to work with, so reach out to three in case one isn’t able to respond to your email.... -
Adenoviral Delivery of CRISPR/Cas9 Aims to Expand Genome Editing to Primary Cells
TypeBlog PostPublishedDec. 10, 2020, 1 p.m....encapsulating the genome is majorly composed of three proteins that can be easily modified in order to...description of their experimental protocol. The three plasmids which have been deposited to Addgene are... -
Capturing a Moment with Marc Zimmer’s “The State of Science”
TypeBlog PostPublishedJuly 30, 2020, 1:15 p.m....and that is what I would call bad science.” The three chapters in this section describe the challenges...case studies, and Zimmer provides several. In Part Three of the book, called simply “Old Science,” he describes... -
Droplet Digital PCR for AAV Quantitation
TypeBlog PostPublishedJan. 16, 2020, 2:18 p.m....analyzed by comparing the threshold fluorescence of the viral DNA to the threshold fluorescence of a standard... -
CRISPR Antimicrobials
TypeBlog PostPublishedMay 3, 2016, 2:30 p.m....antibiotic resistance Most antibiotics target one of three essential processes: cell wall synthesis, DNA replication... Phages could be used to deliver any of the three systems discussed above, with the caveat that phages... -
The Importance of a Fun Workplace: Company Culture at Addgene
TypeBlog PostPublishedSept. 3, 2015, 2:30 p.m....later, Addgene is a company of fifty instead of three, and we are sending hundreds of plasmid requests...enjoyable events for employees. We now have a team of three (myself included) that plan big company events and... -
Four Base Editing Reporters to Monitor and Enrich Editing in Real-time
TypeBlog PostPublishedJuly 7, 2020, 1:15 p.m....individually mutated three codons in eGFP to eliminate fluorescence. This created three inactivated eGFP ... -
Binning Singletons: Tackling Conference Networking When You Don’t Know Anyone
TypeBlog PostPublishedFeb. 18, 2020, 2:15 p.m....Hannigan, Irene Newton, Juliet Johnston, Kat Milligan-Myhre, Kim Dill-McFarland, Lisa Gorski, Mark O. Martin...Griffis, Janet Iwasa, Shawnna Buttery, Kat Milligan-Myhre, and Pragati Chengappa. Financial and moral supporters... -
Viral Production at Addgene
TypeBlog PostPublishedOct. 24, 2023, 1:15 p.m....titers obtained using ddPCR are generally two to three fold higher than those achieved using the standard...vector preparations are added to cells in culture. Three to five days later, cell cultures are inspected ... -
Analyzing CRISPR Editing Results with ICE from Synthego
TypeBlog PostPublishedMay 8, 2018, 1 p.m....analysis will open up a new window with three tabs. Each of the three tabs - Contributions, Indel Distribution... -
Mesothelioma - Causes, Symptoms, and Treatment
TypeBlog PostPublishedDec. 13, 2016, 3:30 p.m....greater the risk of disease) there is no known safe threshold or “safe” exposure level. Unfortunately, the symptoms...important biomarker can be used to distinguish between three groups of people: Those who have mesothelioma ... -
QC Sequencing Technologies at Addgene
TypeBlog PostPublishedJuly 15, 2025, 1:15 p.m....Shovill, apc, and NOVOPlasty. The pipeline contains three de novo assemblers, so if one fails to generate ... plasmid sequence, it moves to the next. If all three attempts fail, the QC team manually checks and assembles... -
Designing Your Chalk Talk for the Academic Job Interview
TypeBlog PostPublishedMarch 12, 2019, 1:37 p.m....you a million dollar startup package. There are three steps to chalk talk preparation: Define your research... Typically, a research vision is broken up into three aims. Two or four aims are also acceptable. Aims... -
Hot Plasmids Spring 2024
TypeBlog PostPublishedMay 23, 2024, 1:15 p.m....the Voltron2 voltage indicator, created by the Schreiter Lab and the GENIE Project at Janelia, are now ...-to-noise ratio and better ability to record subthreshold voltage fluctuations, while having high fidelity... -
Changing Labor Laws Bring Increased Postdoc Wages
TypeBlog PostPublishedOct. 25, 2016, 2:30 p.m....Executive Director, Gary McDowell. On Dec 1st, the threshold at which salaried workers receive overtime payment...either all affected scientists are paid above this threshold or for tracking the hours that these scientists... -
Data Freedom: The Expansion of Data Sharing in Research Publications
TypeBlog PostPublishedAug. 5, 2014, 6:51 p.m....The reason this topic is important to my lab is three-fold. Firstly, we rely on many other labs to teach...Toronto, Canada where he studies protein-serine/threonine kinases implicated in human diseases. His spare...