We narrowed to 332 results for: ICL
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TypeBlog PostPublishedApril 21, 2017, 2:30 p.m....education. Disclaimer: The views presented in this article are those of the author do not represent a formal...politics. Disclaimer: The views presented in this article are those of the author do not represent a formal...having evidence’ on Trump’s wiretap claim The Chronicle of Higher Education: “March for Science” Organizer...
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CRISPR 101: Engineering the Plant Genome Using CRISPR/Cas9
TypeBlog PostPublishedOct. 11, 2016, 2:30 p.m....derived bacterium Agrobacterium tumefaciens as a vehicle to deliver your gene of interest into a target ...cells. Agrobacterium tumefaciens can be used as a vehicle to deliver your gene of interest (GOI) into plant...deliver CRISPR-Cas9 components into plants. Nanoparticles (carbon nanotubes, Kwak et al., 2019), DNA nanostructures... -
Hot Plasmids: Spring 2025
TypeBlog PostPublishedJune 3, 2025, 1:15 p.m....preps in the repository through our Hot Plasmids articles. Here's what you'll find in this post: Glutamate... (Addgene #236081), then generated lentiviral particles in HEK293T cells with pMD2.G (Addgene #12259) ...the viral preps here, and all plasmids from the article! Furlanis, E., Dai, M., Leyva Garcia, B., Tran... -
A Practical Guide to Optimizing AAV DIO and FLEx Vector Expression
TypeBlog PostPublishedNov. 2, 2021, 1:15 p.m.... of recombinase-dependent AAV vectors. In this article, we will discuss some of the causes of off-target...off-target expression just due to more overall viral particles getting into those cells. Therefore, the first...CAG are very strong and may require less viral particles or relatively short expression times, where as... -
Antibodies 101: Introduction to Antibodies
TypeBlog PostPublishedJan. 19, 2021, 2:15 p.m....Crivianu-Gaita 2016). Get more details on ScFvs in this article: Antibodies 101: Single Chain Fragment Variables...concentration. To get even more details, check out our article The Basics of Western Blotting. Figure...experimental samples. Get more details on ELISAs in our article Antibodies 101: ELISA (Enzyme-linked Immunosorbent... -
27 Hot Plasmids from 2016
TypeBlog PostPublishedDec. 22, 2016, 3:03 p.m....repository through our hot plasmids articles. These brief articles highlight the main features and applications...plasmid or set of plasmids. We hope that these articles make it easier for you to find and use the plasmids...tool for the yeast community in their open access article. Mülleder M, et al. F1000Res. PubMed PMID: 27830062... -
Export Control Regulations Lifted for Plasmids Containing Rabies and VSV-G Genes
TypeBlog PostPublishedFeb. 6, 2025, 2:15 p.m.... for producing lentiviral and MuLV retroviral particles. Use in conjunction with a packaging vector such...VSV-G envelope protein for pseudotyping NanoMEDIC particles Akitsu Hotta pHCMV-RabiesG Contains Rabies... -
How Do I Place an Addgene Order Using a PO Number?
TypeBlog PostPublishedFeb. 27, 2020, 2:15 p.m....process not addressed in this article, check out our Help Center article How do I pay by purchase order... -
Searchable and Sortable gRNAs for Your Next CRISPR Experiment
TypeBlog PostPublishedMay 17, 2016, 2:30 p.m....plasmids have been used successfully in peer reviewed articles. If you’re targeting your favorite gene with CRISPR...table has been used effectively in a published article. This blog post will serve as a guide to use our... -
CRISPR Protocol for Genomic Deletions in Mammalian Cell Lines [Video]
TypeBlog PostPublishedFeb. 18, 2015, 3:09 p.m....proud to present a video reprint of the CRISPR article "Generation of Genomic Deletions in Mammalian Cell...Read and download the full protocol from the JOVE article here on Addgene's website and find more CRISPR ... -
New Norepinephrine Indicators: nLightG and nLightR
TypeBlog PostPublishedDec. 12, 2023, 2:15 p.m....norepinephrine in vivo. Nature Methods, 20(9), Article 9. https://doi.org/10.1038/s41592-023-01959-z Mirdita...folding accessible to all. Nature Methods, 19(6), Article 6. https://doi.org/10.1038/s41592-022-01488-1 More... -
Zebrafish as a Model for Behavior: Swimming into the Optogenetic Spotlight
TypeBlog PostPublishedJuly 16, 2019, 12:59 p.m....are typically ion channels that allow charged particles to cross the cell membrane when exposed to light...light. Neuronal firing depends on these charged particles and whether they make the inside of the neuron... -
ReFigure: Save Scientific Figures into Dashboards and Share Your Insights
TypeBlog PostPublishedDec. 13, 2017, 3:20 p.m....could save the figures of interest along with the article metadata, legend and user created notes on a “Pinterest... plugin that allows you to save any image from articles openly available on PubMed Central (also eLife... -
Management for Scientists: Managing vs. Leading
TypeBlog PostPublishedMarch 31, 2015, 4:08 p.m....Management for scientists. We are inundated with articles and books on the topic of leadership. Perhaps ... Business Review 10 Must Reads: On Leadership (Articles on Leadership, also can be purchased individually... -
What the HEK?
TypeBlog PostPublishedApril 18, 2023, 1:15 p.m....HEKs will produce and package viral particles. The viral particles are then filtered to remove any unwanted... -
What Do I Do Now? Academic v. Non-Academic Career Decisions
TypeBlog PostPublishedMarch 9, 2017, 3:40 p.m....From Academic Solos to Industrial Symphonies, an article well worth reading on this topic, the authors state...interfere with a seamless jump into industry.” In his article On Leaving Academe, Terran Lane is asked by his... -
Viral Genome Sequencing - A One Stop Shop for AAV Identity and Serotype Confirmation
TypeBlog PostPublishedApril 23, 2020, 1:15 p.m....has been sparse including only titration of AAV particles and a protein stain to assess purity. For labs...Separation of two types of adeno-associated virus particles containing complementary polynucleotide chains... -
Rewiring Metabolic Circuitry with CRISPR RNA Scaffolds [Video]
TypeBlog PostPublishedApril 7, 2015, 4:21 p.m.... be found here: https://www.addgene.org/browse/article/9593/ Plasmids described in Qi et al. Cell (... be found here: https://www.addgene.org/browse/article/6441/ Resources: An open source library of characterized... -
Unique and persistent IDs for improved reproducibility: Addgene now supports RRIDs and compact identifiers for all plasmids
TypeBlog PostPublishedNov. 20, 2018, 8:17 p.m....link it will take you directly to the original article online. It is also designed to be persistent and...publications that have used the same plasmid through the “Articles Citing this Plasmid” section. Having IDs linked... -
Plasmids 101: Gram Negative and Positive Bacteria
TypeBlog PostPublishedAug. 2, 2022, 1:15 p.m.... https://www.technologynetworks.com/immunology/articles/gram-positive-vs-gram-negative-323007 How the... https://www.technologynetworks.com/immunology/articles/gram-positive-vs-gram-negative-323007 More resources...