...study showed that out of 238 published scientific articles, only 46% could be reproduced. In a recent Nature...notebook requires time and knowledge. In a recent article, we translated our knowledge of electronic lab ...what exactly your lab needs from an ELN (the full article is available here):   Does your lab need to keep...

...derived bacterium Agrobacterium tumefaciens as a vehicle to deliver your gene of interest into a target ...cells. Agrobacterium tumefaciens can be used as a vehicle to deliver your gene of interest (GOI) into plant...deliver CRISPR-Cas9 components into plants. Nanoparticles (carbon nanotubes, Kwak et al., 2019), DNA nanostructures...

... of recombinase-dependent AAV vectors. In this article, we will discuss some of the causes of off-target...off-target expression just due to more overall viral particles getting into those cells. Therefore, the first...CAG are very strong and may require less viral particles or relatively short expression times, where as...

...Crivianu-Gaita 2016). Get more details on ScFvs in this article: Antibodies 101: Single Chain Fragment Variables...concentration. To get even more details, check out our article The Basics of Western Blotting.    Figure...experimental samples. Get more details on ELISAs in our article Antibodies 101: ELISA (Enzyme-linked Immunosorbent...

...repository through our hot plasmids articles. These brief articles highlight the main features and applications...plasmid or set of plasmids. We hope that these articles make it easier for you to find and use the plasmids...tool for the yeast community in their open access article. Mülleder M, et al. F1000Res. PubMed PMID: 27830062...

... for producing lentiviral and MuLV retroviral particles. Use in conjunction with a packaging vector such...VSV-G envelope protein for pseudotyping NanoMEDIC particles Akitsu Hotta pHCMV-RabiesG Contains Rabies...

...process not addressed in this article, check out our Help Center article How do I pay by purchase order...

...plasmids have been used successfully in peer reviewed articles. If you’re targeting your favorite gene with CRISPR...table has been used effectively in a published article. This blog post will serve as a guide to use our...

...proud to present a video reprint of the CRISPR article "Generation of Genomic Deletions in Mammalian Cell...Read and download the full protocol from the JOVE article here on Addgene's website and find more CRISPR ...

...norepinephrine in vivo. Nature Methods, 20(9), Article 9. https://doi.org/10.1038/s41592-023-01959-z Mirdita...folding accessible to all. Nature Methods, 19(6), Article 6. https://doi.org/10.1038/s41592-022-01488-1 More...

...are typically ion channels that allow charged particles to cross the cell membrane when exposed to light...light. Neuronal firing depends on these charged particles and whether they make the inside of the neuron...

...could save the figures of interest along with the article metadata, legend and user created notes on a “Pinterest... plugin that allows you to save any image from articles openly available on PubMed Central (also eLife...

...Management for scientists.  We are inundated with articles and books on the topic of leadership. Perhaps ... Business Review 10 Must Reads: On Leadership (Articles on Leadership, also can be purchased individually...

...has been sparse including only titration of AAV particles and a protein stain to assess purity. For labs...Separation of two types of adeno-associated virus particles containing complementary polynucleotide chains...

...HEKs will produce and package viral particles. The viral particles are then filtered to remove any unwanted...

...From Academic Solos to Industrial Symphonies, an article well worth reading on this topic, the authors state...interfere with a seamless jump into industry.” In his article On Leaving Academe, Terran Lane is asked by his...

... be found here: https://www.addgene.org/browse/article/9593/ Plasmids described in Qi et al. Cell  (... be found here: https://www.addgene.org/browse/article/6441/ Resources: An open source library of characterized...

...link it will take you directly to the original article online. It is also designed to be persistent and...publications that have used the same plasmid through the “Articles Citing this Plasmid” section. Having IDs linked...

... https://www.technologynetworks.com/immunology/articles/gram-positive-vs-gram-negative-323007 How the... https://www.technologynetworks.com/immunology/articles/gram-positive-vs-gram-negative-323007 More resources...

... Stacey. Opinions on the apps discussed in the article are those of the guest blogger and do not necessarily...think it’s safe to assume that anyone reading this article has a smartphone nearby. We tailor these devices...