Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 316 results for: Mut

Showing: 81 - 100 of 316 results

  1. Targeting HIV-1 with CRISPR: Shock and Kill or Cut it Out?

    Type
    Blog Post
    Published
    May 10, 2016, 2:30 p.m.
    ...and CRISPR/Cas9 have also been used to create mutations in genes essential to HIV-1 replication. However...CRISPR therapies through PAM or seed sequence mutations. Multiple gRNAs could be used to combat this problem...LTRs or essential genes. Many of these escape mutations were located near the Cas9 cut site, leading Wang...escape occurs in an animal model. If these escape mutations are frequent, the dCas9-mediated “shock and kill... S, Wainberg MA, Liang C. CRISPR/Cas9-Derived Mutations Both Inhibit HIV-1 Replication and Accelerate ...

  2. Recombinase-based State Machines Enable Order-dependent Logic in vivo

    Type
    Blog Post
    Published
    July 28, 2016, 2:30 p.m.
    ...which regulatory proteins, signaling molecules, mutations, and environmental conditions drive certain cellular...arrange attB-attP pairs on a register to record all permuted substrings of a set of chemicals. Specific examples...arrangements that should be able to record all permuted substrings of up to seven chemical inputs are ...a population to correctly record each possible permuted substring of the inputs, and that five hour inductions...percent of cells to correctly record each possible permuted substring of inputs (see Figure S9 of our paper...

  3. PiggyBac-ing Through the Genome Editing Field

    Type
    Blog Post
    Published
    May 31, 2016, 3:30 p.m.
    ...completely seamless manner, leaving no sequences or mutations behind. Furthermore, piggyBac™ offers a large ...is constitutively-active or a dominant-negative mutant. Reducing the copy number can also mitigate the...excision-only piggyBac™ transposase (PBx), which contains mutations making it competent for excision, but defective...primarily two other ways to create specific point mutations by gene editing. First, instead of using piggyBac...piggyBac™ transposase or hyperactive transposase mutants [7], it is generally not as efficient as Cre recombinase...

  4. A History of Genome Engineering in Popular Culture

    Type
    Blog Post
    Published
    Feb. 25, 2020, 2:15 p.m.
    ...genome and are inefficient at introducing point mutations. In 2016, the researchers at the Broad Institute... envisioned a new strategy to introduce point mutations in the genome. They fused a catalytically impaired...a tRNA deaminase enzyme, TadA) to induce a A→G mutation at the target genomic loci. Together, CBE and ...the prime editor can install all possible point mutations, delete short stretches of DNA and even insert...doi.org/10.1038/nprot.2013.143 Seligman LM (2002) Mutations altering the cleavage specificity of a homing ...

  5. Fluorescent Proteins 101: Photoactivatable Fluorescent Proteins

    Type
    Blog Post
    Published
    April 25, 2017, 2:30 p.m.
    ...from Jellyfish (Aequorea victoria) GFP (wtGFP) by mutating Threonine 203 to Histidine (T203H) (1). wtGFP ... when excited with 488nm blue light. The T203H mutation in PA-GFP generates a chromophore population that...PA-FPs by performing several rounds of random mutagenesis screening for enhanced RFP variants. PA-mCherry...negative” photoswitching. In 2008, scientists used mutagenesis screens to find a version of Dronpa that doesn't... is a monomeric, reversible PA-FP derived from mutating Dronpa (T59M / V60A / N94I / P141L / G155S / V157G...

  6. 28 Hot Plasmid Technologies from 2015

    Type
    Blog Post
    Published
    Dec. 23, 2015, 3:30 p.m.
    ...carried out five rounds of random mutagenesis on a library of Arch mutants screening for improved brightness...brightness. The brightest mutants were then further mutagenized and screened for speed and voltage sensitivity...retention insensitivity to other ions In BRET, A mutant Renilla luciferase, catalyzes the oxidation of ...transferred to the acceptor fluorophore, a circularly permuted Venus. In the case of pHlash, the signal from ... SalI sites must be removed by site-directed mutagenesis in order to use the module as a building block...superior to Arch). Both QuasAr1 and QuasAr2 possess mutations for improved voltage sensitivity and speed, and...

  7. How to Deposit Your Plasmids with Addgene

    Type
    Blog Post
    Published
    Feb. 27, 2024, 2:15 p.m.
    ...Relevant mutations/deletions" box to describe any mutations within your insert using standard mutation notation...fusion proteins contained in the plasmid, any mutations, and the intended purpose of the plasmid. Once...functional consequences of that change. For example, "Mutated Aspartate 123 to Lysine (D123K), lowers biosensor...

  8. CRISPR 101: Cas9 Nickase Design and Homology Directed Repair

    Type
    Blog Post
    Published
    March 15, 2018, 12:59 p.m.
    ...By mutating one of two Cas9 nuclease domains, researchers created the CRISPR nickase. Nickases create...SpCas9 nickases as examples for this post. The D10A mutation inactivates the RuvC domain, so this nickase cleaves... only the target strand. Conversely, the H840A mutation in the HNH domain creates a non-target strand-...there are no available guides close to the desired mutation site. Quick tips for nickase design Ready to ...

  9. CRISPR 101: Non-Homologous End Joining

    Type
    Blog Post
    Published
    April 16, 2015, 3:45 p.m.
    ...processing of DNA ends tends to be the point where mutations are introduced. Repair of Cas9-induced breaks ...about a two-thirds chance of causing a frameshift mutation. Of some importance, the deletion can be less ...double nickase” strategy vastly reduces breaks and mutations at off-target sites. Christina Mork contributed...Persistence of a Chromosome Break Diminishes Its Mutagenic Potential. PLoS Genet 5:e1000683 . https://doi.org...

  10. Magnetic Control of Proteins: More than a Dream

    Type
    Blog Post
    Published
    Sept. 24, 2024, 1:15 p.m.
    ...more rounds of mutagenesis, they eventually found a version of AsLOV2 with five mutations (C450P, L496V... AsLOV2. After several rounds of semi-random mutagenesis and screening, the EGFP and mScarlet variants... and ΔF/F of MagLOV, a variant of AsLOV2 with mutations C450P, L496V, Q513K, G528K, D540M, in response...

  11. CRISPR 101: Mammalian Expression Systems and Delivery Methods

    Type
    Blog Post
    Published
    Sept. 24, 2015, 2:30 p.m.
    ...expression? Are you trying to create a single point mutation? Do you want to create a fusion with a reporter...generating knockouts or knock-ins, or introducing mutations and tags, while a catalytically dead dCas9 can... Base editors can help you make precise point mutations near the gRNA target site. Choose an appropriate...repair (HDR) template to create knock-ins, point mutations, or to add a tag, you will also need to deliver...

  12. Plasmids 101: Choosing an Antibiotic Resistance Gene

    Type
    Blog Post
    Published
    Oct. 10, 2023, 1 p.m.
    ...cleavage in human cells. Mutagenesis, 20(2), 111–114. https://doi.org/10.1093/mutage/gei016I Resources on ...when not 100% inhibited, meaning it will induce mutations in the host/plasmid DNA during selection if it...good choice for experiments in which off-target mutations are undesirable. Pros: Works across many species...

  13. 15 Hot Plasmids from 2017

    Type
    Blog Post
    Published
    Jan. 9, 2018, 3:02 p.m.
    ... used a combination of deliberate and random mutagenesis to isolate the best RFP. They targeted known ...researcher’s locus of interest. NHEJ- or HDR-induced mutations in ATP1A1 can render cells resistant to the small...improves editing efficiency. Importantly, the point mutations created by Agudelo et al. prevent ouabain binding...properties of the individual Cas9. Kim et al. also mutagenized the cytidine deaminase portion of the base editor...FLAG-tagged TZAP constructs (wild-type and truncation mutants), gRNAs used for TZAP gene editing, and a Hi6xs-MBP...

  14. CRISPR 101: Any Base Transversion Editors

    Type
    Blog Post
    Published
    April 1, 2025, 1:15 p.m.
    ...this promising start, the team used rational mutagenesis to produce the adenine transversion editor AYBEv3... edits. Their final tool, ACBE-Q (for an N108Q mutation in the deaminase), produced an average A → C product...and ABEs, they could be useful for saturation mutagenesis, and they provide a strong proof of concept for...empty space. Over several rounds of rational mutagenesis, the team progressed to gGBEv6.3, which was capable...

  15. How to Write a Scientific Review Article

    Type
    Blog Post
    Published
    Feb. 16, 2017, 3:30 p.m.
    ...studies that reported mutations in that protein (including how frequently a mutation was found and the size...frequently each member of a class of proteins was mutated, as reported in various studies. We made a table...could easily see how frequently each protein was mutated, you could see how big the studies were, and you...

  16. To Codon Optimize or Not: That is the Question

    Type
    Blog Post
    Published
    Nov. 12, 2020, 2:15 p.m.
    ...codon bias. The idea is that scientists can make mutations throughout a gene of interest based on an organisms...) to name a few. This could affect synonymous mutations which have even been linked to some diseases. ...human genes contain a region where a synonymous mutation can be harmful (Sauna and Kimchi-Sarfaty, 2011... Understanding the contribution of synonymous mutations to human disease. Nat Rev Genet 12:683–691. https...

  17. CRISPR 101: Which Cas9 Do I Choose for My CRISPR Experiment?

    Type
    Blog Post
    Published
    Oct. 13, 2022, 1:45 p.m.
    ...engineered by introducing Cas9-mediated frameshift mutations in the gene of interest. The genomic target for...for Cas9 and the introduction of this mutation can be any site early in the coding region of the gene ... experiments may require the introduction of a mutation at a specific site e.g., deleting the catalytic...modification at a defined amino acid to mimic a patient mutation or maybe to disrupt a catalytic residue. Good ...

  18. New CRISPR Tools: Cas7-11 and PASTE

    Type
    Blog Post
    Published
    Feb. 17, 2022, 2 p.m.
    ...patient's specific mutations. With a drag and drop approach, however, the specific mutations wouldn’t matter...which can be caused by thousands of different mutations, all found in the same gene. If we only have small...matter – the mutated copy could be replaced by simply dragging and dropping in a functional copy of the entire...

  19. The PAM Requirement and Expanding CRISPR Beyond SpCas9

    Type
    Blog Post
    Published
    Aug. 20, 2020, 2:30 p.m.
    ...These off-target sequences may be unintentionally mutated along with the desired target locus. The PAM sequence...positive selection screens in bacteria to identify mutants of S. pyogenes Cas9 that were able to cleave target...PAM binding specificity: SpCas9 variant Mutations (relative to SpCas9) PAM sequence D1135E...human genome. Another variant, xCas9 3.7 has 7 mutations found in the REC2, REC3, and PAM interacting domains...

  20. Plasmids 101: E. coli Strains for Protein Expression

    Type
    Blog Post
    Published
    Feb. 10, 2015, 3:06 p.m.
    ... with sub-optimal codon usage (Table 1) A few mutations are common to all or most expression strains to...levels including:  ompT: Strains harboring this mutation are deficient in outer membrane protease VII, ...methylate DNA. dcm: Similarly, strains with this mutation are unable to methylate cytosine within a particular... of unstable proteins Tuner (DE3)   Contains mutated lac permease whch allows for linear control of ...
Showing: 81 - 100 of 316 results