We narrowed to 290 results for: Pol
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TypeBlog PostPublishedAug. 23, 2022, 1:15 p.m....biochemistry, microbiology, epidemiology, and bioanthropology. Studying human health science made me happy...greater science literacy and education have more polarized beliefs on controversial science topics. Proceedings..., L. L., Braman, D., & Mandel, G. (2012). The polarizing impact of science literacy and numeracy on perceived...
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A Look Back at One Year of Plasmid Sharing for COVID-19 Research
TypeBlog PostPublishedJuly 12, 2021, 1:15 p.m.... the reverse transcriptase, protease, and Bst polymerase constructs from their assay to Addgene. Drew...expressing MMLV reverse transcriptase and Taq polymerase that can be used in a one-step RT-qPCR reaction...Nguyen AW, Park J, Wang N, Amengor D, Lavinder JJ, Ippolito GC, Maynard JA, Finkelstein IJ, McLellan JS (2020... -
Savvy Advocates Needed to Navigate a Scientific Enterprise in Flux
TypeBlog PostPublishedSept. 13, 2016, 2:30 p.m..... The topic of unionization continues to be a polarizing one. High-profile universities remain outspoken... panelists including: Kate Stoll, PhD - Senior Policy Advisor MIT Washington Office Ben Corb - Director... in the intersection between science and public policy. Follow David on Twitter @driglar. References... -
How-to: 5 Steps to a Great Panel Discussion
TypeBlog PostPublishedOct. 22, 2014, 1:57 p.m....advantage of the program. Start the program by polling the audience with a few questions. Who is out there...in response. I am always ready with some written poll questions to get things started. Give a couple of...want to take over? It is the moderator's job to politely but firmly get them to stop talking and redirect... -
PCR Applications for Copy Number Variation Assays
TypeBlog PostPublishedApril 26, 2022, 1:15 p.m....Addgene blog Droplet Digital PCR for AAV QuantificationPolymerase Chain Reaction Overview and Applications...Applications Resources on Addgene.org Protocol: Polymerase Chain Reaction (PCR) Protocol: How to Design a primer...SYBR Green I and TaqMan quantitative real-time polymerase chain reaction methods for the determination ... -
Plasmids 101: 5 factors to help you choose the right cloning method
TypeBlog PostPublishedAug. 21, 2018, 12:31 p.m....fragments are then combined with an exonuclease, a polymerase, and a DNA ligase to carry out a pooled set of...topoisomerase I cut site, toposisomerase I, and a taq polymerase-generated insert. When mixed, topoisomerase I... could also be a great choice, because the T4 polymerase needed for this method is relatively inexpensive... -
Plasmids 101: Protein Expression
TypeBlog PostPublishedJune 7, 2018, 1:17 p.m.... can then be translated at the ribosome into polypeptide chains that make up the primary structure of ...purified for use in molecular biology research (eg. polymerases and other enzymes might be purified and used ...translation machinery. These include ribosomes, RNA polymerase, tRNAs, ribonucleotides and amino acids. Cell-free... -
Great Results Start with Great Standard Curves
TypeBlog PostPublishedAug. 15, 2024, 1:15 p.m....versus the response and use that information to extrapolate the concentration of an unknown sample. For example...sigmoidal. Once the trendline is calculated, you can extrapolate the concentration of the unknown sample based...sample is determined by using the trendline to extrapolate its x value, 0.963 mg/mL, from its y value, 0.651... -
Plasmids 101: Colony PCR
TypeBlog PostPublishedMay 12, 2016, 2:30 p.m....set up a standard PCR reaction (primers, dNTPs, polymerase) using the supernatant of lysed bacteria as template...standard PCR reaction: combine template, primers, polymerase, and dNTPs and then incubate with a standard ...heating step of the PCR reaction. A standard Taq polymerase is sufficient. Controls: Controls can make ... -
What's Your Organism? Expanding Genomic Tools via the NSF EDGE Program
TypeBlog PostPublishedMay 2, 2019, 12:41 p.m....No transformation techniques are available yet. Polistes (Amy Toth) These wasps are like the ones that ...Crepidula atrasolea juvenile, light microscope with polarizing filter. Development is direct in this species...actually silence this gene with RNAi to make a polygamous species monogamous. Anolis sagrei (Doug Menke... -
Viral Vectors 101: Pseudotyping
TypeBlog PostPublishedApril 20, 2021, 2:30 p.m....which contains a promoter, the envelope gene, and a polyA tail. Figure 1: Lentiviral production uses... inactivated by the complement system in serum (DePolo et al., 2000). Other envelope proteins for pseudotyping...–398 . https://doi.org/10.2174/1566523054546224 DePolo NJ, Reed JD, Sheridan PL, Townsend K, Sauter SL... -
Viral Production at Addgene
TypeBlog PostPublishedOct. 24, 2023, 1:15 p.m....Samples of viral preparations are subjected to polyacrylamide gel electrophoresis (PAGE) followed by silver...centrifugation and then by filtration through a 0.45 µm polyethersulfone (PES) membrane. The preparations are then ...lentiviral preparation by precipitation in 10% polyethylene glycol (PEG) followed by centrifugation. Precipitated... -
Four Ways to Package Transgenes That Exceed the Size Limit of Adeno-associated Virus
TypeBlog PostPublishedSept. 1, 2020, 1:15 p.m....Iodice C, Polishchuk EV, Sommella A, Colecchi L, Rossi S, Simonelli F, Giunti M, Bacci ML, Polishchuk RS, Auricchio...Marrocco E, Rossi S, Giunti M, Palfi A, Farrar GJ, Polishchuk R, Auricchio A (2013) Effective delivery of large... -
Deep Mutational Scanning with One Pot Saturation Mutagenesis
TypeBlog PostPublishedFeb. 22, 2017, 3:30 p.m....with a “sloppy” version of PCR that results in a polymerase error rate of up to 2% per nucleotide position...modifications: 1) increased concentration of Taq polymerase, 2) increased PCR extension time, 3) increased...degenerate primers and the high-fidelity Phusion polymerase are used to introduce point mutations into the... -
10 Steps to a Perfect Science Talk
TypeBlog PostPublishedAug. 23, 2016, 2:30 p.m....of useful, entertaining science lab, career, and policy content. Watch Joanne give her "Not" Networking...almost anyone with enough practice. I always take a poll of scientists in training to see how many in my ...other ways to engage the audience. Take an informal poll by asking for a show of hands on a particular question... -
RNA Interference in Plant Biology: New Tools for an Old Favorite
TypeBlog PostPublishedOct. 27, 2020, 1:15 p.m....from Worcester Polytechnic Institute where he investigated how plants achieve polarized cell growth in... in Biology and Biotechnology from Worcester Polytechnic Institute. What is RNAi? The loss-of-function... -
Antibodies 101: Antibody Engineering and Directed Evolution
TypeBlog PostPublishedAug. 5, 2025, 1:15 p.m....high-fidelity polymerase to ensure accuracy. In this case, you would want a low-fidelity polymerase, like Taq...forcing sequence diversity using error-prone polymerases, you force sequence diversity using a pool of... -
A Primer on Optogenetics: Introduction and Opsin Delivery
TypeBlog PostPublishedSept. 10, 2015, 2:30 p.m....cation channel, activation of which results in depolarization and subsequent firing of an action potential...results in efflux of chloride ions, subsequent hyperpolarization, and inhibition of action potential generation...spectrum as HR and also results in membrane hyperpolarization but unlike HR, this is achieved through hydrogen... -
CRISPR 101: Cytosine Transversion Editors
TypeBlog PostPublishedMarch 25, 2025, 1:15 p.m....proteins whose fusion improved the CGBE: DNA polymerase D2 (POLD2), exonuclease 1 (EXO1), and RNA binding ...rXRCC1, or the DNA binding and lyase domain of DNA polymerase β (PB) (Chen et al., 2021). Both of these fusion... -
Celebrating the 2024 Nobel Prize in Chemistry
TypeBlog PostPublishedOct. 17, 2024, 1:15 p.m....with BioRender.com. Top7 gets its name from “topology of protein” diagrams, which show the interactions...seen so far!). They started by designing a novel topology, then iteratively generated 3D models and amino...