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We narrowed to 333 results for: caps

Showing: 81 - 100 of 333 results

  2. Back to Bacteria: CRISPR gRNA Multiplexing Using tRNAs

    Type
    Blog Post
    Published
    June 2, 2015, 6:06 p.m.
    ... will be necessary to improve its multiplexing capacity. Multiplexing is key due to the natural redundancy...multiplexing in systems with limited packaging capacity. Still confused about the various tRNA expression...References Boosting CRISPR/Cas9 multiplex editing capability with the endogenous tRNA-processing system. Xie...

  4. 27 Hot Plasmids from 2016

    Type
    Blog Post
    Published
    Dec. 22, 2016, 3:03 p.m.
    ... below:   ID Cv (Capsid) FLAG Tag Terminus 79629 (N) and 79628 (C) Cv (capsid) N and C 79633 (N...system has a much larger cargo capacity than lentivirus and is capable of efficiently creating stable ...homology (usually 30-40bp overlaps). To expand the capabilities of Gibson assembly and make a more modular platform...

  5. Savvy Advocates Needed to Navigate a Scientific Enterprise in Flux

    Type
    Blog Post
    Published
    Sept. 13, 2016, 2:30 p.m.
    ...Recent developments promise to further change the landscape facing all scientists, both young and established...potential to profoundly shift the academic science landscape: the treatment of trainee scientists as workers...shifts caused to the practical and philosophical landscape of the scientific enterprise will provide challenges...

  6. Cpf1 Update: Comparison to Cas9 and NgAgo

    Type
    Blog Post
    Published
    July 14, 2016, 2:30 p.m.
    ...counterparts, so they will be easier to deliver in low-capacity vectors like AAV. AAV (adeno-associated viral ...tissues. However, these vectors have a low genetic capacity of ~4.5 kb. At 4.1 kb, SpCas9 barely fits into...understand where Cpf1 fits into the genome editing landscape. References 1. Kim D, Kim J, Hur JK, Been KW...

  7. Experimenting in an Open Source lab: from CRISPR to Cats

    Type
    Blog Post
    Published
    July 23, 2015, 2:30 p.m.
    ...biology of the worm into a computer model that recapitulates how molecular biology impacts worm locomotion...mutant worm. Like many academic labs, we have the capability to study many different mutants because the Caenorhabditis...and cobble together quite a diverse array of capabilities. So don’t throw old equipment away until you...

  8. AAV Vector Quality Control: Going the Extra Mile with NGS

    Type
    Blog Post
    Published
    Sept. 12, 2017, 1:44 p.m.
    ... to the establishment of in-house sequencing capabilities and Seqwell’s plexWell technology, we can now...small predetermined seed sequences unique to the capsid plasmid. Our recombination software looks for and... Moullier P, Salvetti A (2005) Evidence for Encapsidation of Prokaryotic Sequences during Recombinant ...

  9. An Introduction to Adenovirus

    Type
    Blog Post
    Published
    Oct. 3, 2023, 1:15 p.m.
    ... E1 region). The space in the viral particle’s capacity that is made available by this deletion from the... (and their structural proteins, which would encapsidate new virions) are not expressed. Note that replication-competent...genome, and adenoviral vectors all have a genomic capacity that is much higher than other viral vectors (...

  10. Viral Vectors 101: The Retroviral Lifecycle

    Type
    Blog Post
    Published
    July 27, 2023, 1:15 p.m.
    ...transcriptase are packaged (Wu et al., 1997) inside of the capsid . This is useful for viral reproduction, and for...for reverse transcriptase (RT) is present in the capsid, as the engineered, recombinant genome is (thankfully...mouse embryo cell lines in which each cell has the capacity to be activated to produce infectious murine leukemia...

  11. INTEGRATE: Bacterial Genome Engineering Using CRISPR-Transposons

    Type
    Blog Post
    Published
    Dec. 7, 2020, 2:53 p.m.
    ...DNA transposons are ubiquitous genetic elements capable of spreading within and between genomes, and have...transposition. By natively combining the DNA integration capabilities of transposases and the target programmability...large DNA cargos into bacterial genomes. This new capability was named INTEGRATE (Insertion of Transposable...

  12. Overcoming the AAV Size Limitation for CRISPR Delivery

    Type
    Blog Post
    Published
    Sept. 16, 2020, 1 p.m.
    ...AAV vector is challenging due to its packaging capacity of AAV (~4.7 kb). While this approach has been...separate AAV vectors, increasing overall packaging capacity but necessitating purification and co-infection...-AAV toolbox that retains the gene-targeting capabilities of full-length SpCas9. This set of plasmids ...

  13. Hot Plasmids: Summer 2024

    Type
    Blog Post
    Published
    Aug. 27, 2024, 1:15 p.m.
    ...that combines enrichment and imaging of proteins captured on magnetic beads (Arimura et al., 2024). In MagIC-cryo-EM...GFP fusion in this proof-of-concept study) are captured on streptavidin-coated magnetic beads through ...bundle; SAH: 60-nm single alpha helix) and target capture module (SpyTag-GFP nanobody) assembled on beads...

  14. Important Considerations When Using AAVs

    Type
    Blog Post
    Published
    June 13, 2017, 2:30 p.m.
    ...interest. Although they have a small packaging capacity, AAV vectors were suitable for our needs for the...Joshua C., and Richard J. Samulski. "Packaging capacity of adeno-associated virus serotypes: impact of...recombinant adeno-associated viral vectors: packaging capacity and the role of rep proteins in vector purity....

  15. Quick Guide to All Things Lentivirus

    Type
    Blog Post
    Published
    March 21, 2017, 2:30 p.m.
    ...of interest as well as sequences necessary to encapsulate that insert into a viral particle (the packaging...cell culture as lentivirus-derived vectors are capable of transducing certain quiescent or terminally ...ELISA dosing method detects the expression of viral capsid antigen p24, the FACS dosing method correlates ...

  16. Antibodies 101: Flow Cytometry

    Type
    Blog Post
    Published
    July 20, 2021, 1:15 p.m.
    ...sheath fluid and transported through tubes or capillaries to a laser. As the cells move through the fluidics...different directions. The degree of scatter is captured by detectors and provides useful information about...fluorochrome, emits a fluorescent signal that is captured by detectors. The strength of the emitted photons...

  17. Illuminating Choices: A Guide to Selecting Fluorescent Dyes and Ligands

    Type
    Blog Post
    Published
    March 21, 2024, 1:15 p.m.
    ..., brighter, and specific dyes, expanding the capabilities of fluorescence-based techniques in biotechnology...Fluor HaloTag Ligands expand the self-labeling capabilities of this platform. The Janelia Fluor ligands .../doi.org/10.1007/s41048-017-0037-8 Lucidi, M., Capecchi, G., Visaggio, D., Gasperi, T., Parisi, M., Cincotti...

  18. Which Fluorescence Microscopy Technique is Best for Me?

    Type
    Blog Post
    Published
    Oct. 10, 2017, 1:57 p.m.
    ...entire field of view is excited at one time and captured in a single camera exposure. This is much faster...out-of-focus light. The most difficult characteristic to capture in these types of samples is their fast movements...imaging, the light microscope was limited in its capacity to resolve closely lying structures. This is because...

  19. Selecting Your Plasmid Purification Kit

    Type
    Blog Post
    Published
    May 27, 2025, 1:15 p.m.
    ...to increase your output. But, exceeding a kit’s capacity can actually reduce your ultimate plasmid yield...ensure proper lysis, but you will still face column capacity limitations since the columns can be “clogged”...magnet as you scale up. Table 1: Standard Prep Kit Capacities* Prep size Amount of culture Approximate ...

  20. CRISPR 101: Cytosine Transversion Editors

    Type
    Blog Post
    Published
    March 25, 2025, 1:15 p.m.
    ...Cas9 fused to an engineered adenosine deaminase capable of chemically converting adenosine (A) to inosine.... They also developed a machine learning model capable of predicting editing outcomes of their various..., they added the pioneer factor SOX2, which is capable of binding to and remodeling inaccessible chromatin...
Showing: 81 - 100 of 333 results