We narrowed to 151 results for: cycs

1. Meet the New Addgene Board Members!

   Type

   Blog Post

   Published

   April 28, 2020, 1:14 p.m.

   ...MIT. “I read some of the seminal papers on cell cycle discovery from Lee Hartwell and Paul Nurse,” Stegmeier...

2. Behind-the-scenes of the Isolation of the Thermostable IgnaviCas9 From a Yellowstone Hot Spring

   Type

   Blog Post

   Published

   Nov. 12, 2019, 2 p.m.

   ... with a freezer wired to a charger and two deep cycle batteries for power so the samples could be kept...

3. Hot Plasmids and Viral Preps - September 2021

   Type

   Blog Post

   Published

   Sept. 21, 2021, 1:15 p.m.

   ...the expression of fluorescent proteins and oxytetracycline enzymes. Next, this system was used to recreate...

4. Plasmids 101: TOPO Cloning

   Type

   Blog Post

   Published

   Oct. 27, 2016, 2:30 p.m.

   ... to include extra extension time after the last cycle of PCR to make sure that the "A" gets added to all...

5. New and Upcoming Viral Vectors - June 2019

   Type

   Blog Post

   Published

   June 18, 2019, 1:15 p.m.

   ...  AAV5 ppAAV-CKIIa-stGtACR1-FusionRed New tetracycline transactivators Plasmid Serotype Name 99120...

6. Scientists Map the SARS-CoV-2-Human Interaction Network

   Type

   Blog Post

   Published

   April 9, 2020, 1:15 p.m.

   ...viruses rely on the help of human proteins in their lifecycle, identifying these potential human helpers of ...

7. A Quick Guide to a Career in Software Product Management

   Type

   Blog Post

   Published

   Nov. 24, 2020, 2:15 p.m.

   ... the users, gather more feedback, and start the cycle over again to refine the solution, or to come up...

8. Fluorescent CRISPR Reporters: SRIRACCHA and GEmCherry2

   Type

   Blog Post

   Published

   May 26, 2020, 1:15 p.m.

   ...Cas9 expression is activated using 4-OH-T and doxycycline. A gRNA that targets your desired gene (whose...

9. The Many Reasons for Irreproducible Research- and a Vaccine to Eradicate It

   Type

   Blog Post

   Published

   Jan. 30, 2020, 2:15 p.m.

   ... their study plans at a point in the research lifecycle when suggestions can be incorporated into the ...

10. Opto-Nanobodies: Using Light to Manipulate Cell Signaling and Protein Purification

    Type

    Blog Post

    Published

    Nov. 19, 2019, 2:08 p.m.

    ...OptoNBs. OptoNB coated beads were then imaged while cycling between dark and blue light which revealed a blue...

11. Sweating the Small Stuff: Details in the Lab

    Type

    Blog Post

    Published

    Feb. 8, 2022, 2:15 p.m.

    ... a chance to break what can be a rather vicious cycle.  Everybody’s Different   The truth is, there is...

12. 7 Tips to Secure a STEAM Internship This Summer

    Type

    Blog Post

    Published

    Feb. 2, 2021, 2:15 p.m.

    .... In order to gain a competitive advantage this cycle, consider the advice below:  1. Eligibility check...

13. Viral Vectors 101: Gamma-Retroviral Packaging Systems

    Type

    Blog Post

    Published

    Aug. 6, 2024, 1:15 p.m.

    ... blog Viral Vector Elements The Retroviral Life Cycle Popular Retroviral Vectors and Their Use in Research...

14. Viral Vectors 101: Viruses as Biological Tools

    Type

    Blog Post

    Published

    March 21, 2023, 1:15 p.m.

    ...to produce more virus and continue the infection cycle. Depending on the type of virus used, the DNA (or...

15. Cancer, Inflammation and Immunity - Harnessing the Body’s Defenses to Fight Cancer

    Type

    Blog Post

    Published

    July 6, 2017, 1:59 p.m.

    ...PMID: 28495700. 4. Zelenay, Santiago, et al. "Cyclooxygenase-dependent tumor growth through evasion of immunity...

16. Screening for Successful Genome Editing with Digital PCR

    Type

    Blog Post

    Published

    March 30, 2017, 2:30 p.m.

    ...copies of the target DNA. Conventional thermal cycling is then performed to amplify the target using PCR...

17. Protein Tagging with CRISPR/Cas9: A Conversation with Mendenhall and Myers

    Type

    Blog Post

    Published

    July 28, 2015, 2:30 p.m.

    ...toward this goal for years as members of the ENCyclopedia of DNA Elements Project (ENCODE), an NIH-funded...

18. Advice for Starting a Biotech Company from a Grad Student turned Entrepreneur

    Type

    Blog Post

    Published

    July 10, 2014, 7:17 p.m.

    ...Productize, (B) Roll out to market, and (C) Break even cycles. Work hard. Work fast. Be flexible. Be quick at...

19. RaPID Detection of RNA-protein Interactions

    Type

    Blog Post

    Published

    Aug. 16, 2018, 12:40 p.m.

    ...untranslated regions (UTRs) to aid in the viral life cycle. Ramanathan et al. used RaPID to see what host proteins...

20. It’s Bioelectric! An Exciting Interdisciplinary Field of Research

    Type

    Blog Post

    Published

    Oct. 29, 2019, 2:52 p.m.

    ...upon activation. p-mcherry-C1-iC++_CA - A slow cycling (open for minutes) step-function artificial anion...