We narrowed to 129 results for: itch

1. Synthetic Photobiology: Optogenetics for E. coli

   Type

   Blog Post

   Published

   Sept. 8, 2015, 2:30 p.m.

   ...References Refactoring and optimization of light-switchable Escherichia coli two-component systems. Schmidl...

2. Fluorescent CRISPR Reporters: SRIRACCHA and GEmCherry2

   Type

   Blog Post

   Published

   May 26, 2020, 1:15 p.m.

   ...https://doi.org/10.1038/nbt.2842 Wen Y, Liao G, Pritchard T, Zhao T-T, Connelly JP, Pruett-Miller SM, Blanc...

3. GPCRs: How Do They Work and How Do We Study Them?

   Type

   Blog Post

   Published

   Feb. 11, 2021, 2:15 p.m.

   ...Heterotrimeric G proteins are important molecular switches in signal transduction pathways and consist of...

4. Viral Vectors 101: Biosensors

   Type

   Blog Post

   Published

   July 6, 2023, 1 p.m.

   ...calcium. This includes FRET-based sensors such as Twitch. While calcium is king in the sensor world, there...

5. Plasmids 101: Sequence and Ligation Independent Cloning (SLIC)

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   Blog Post

   Published

   Sept. 26, 2024, 1:15 p.m.

   ...first occurrence of the chosen nucleotide, T4 will switch to polymerizing and then stall due to the absence...

6. Flow Cytometry Readouts: Yes, No, and Everything in Between

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   Blog Post

   Published

   Aug. 20, 2024, 1:15 p.m.

   ...obvious in a dot plot. If that is the case for you, switching to a contour plot should indicate where the two...

7. A Deep Dive into BioBuilder

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   Blog Post

   Published

   Oct. 11, 2017, 1:44 p.m.

   ...exhibit projects at any stage of completion, from pitch to prototype. Last year’s BioBuilderClub teams’ ...

8. Protein Tagging with CRISPR/Cas9: A Conversation with Mendenhall and Myers

   Type

   Blog Post

   Published

   July 28, 2015, 2:30 p.m.

   ...their favorite selection cassette. They could also switch from FLAG to GFP or a different epitope. Addgene...

9. Channelrhodopsins with improved light sensitivity for minimally-invasive optogenetics

   Type

   Blog Post

   Published

   Nov. 3, 2020, 2:15 p.m.

   ...lets you fire neurons with the flick of a light switch. Neurons are not typically persuaded to fire when...

10. Oh, The Places You Can Go: Careers in Science Communication - Writing for a Research Institute

    Type

    Blog Post

    Published

    Feb. 2, 2018, 3:20 p.m.

    ...do a little bit of work for one client and then switch to doing something else. You’re never really integrated...

11. Choosing the B(right)est Fluorescent Protein: Photostability

    Type

    Blog Post

    Published

    June 8, 2017, 2:30 p.m.

    ...Shaner et al, 2008; Bindels et al, 2017) and photoswitching (Kremers et al, 2009), which usually are undesired...

12. CRISPRainbow and Genome Visualization

    Type

    Blog Post

    Published

    Feb. 28, 2017, 3:30 p.m.

    ...but a newly expressed standard length gRNA would “switch” the system and induce genome editing. Such a system...

13. Plasmid-based Recombinant Monoclonal Antibodies: What They Are and Why You Should Be Excited About Them

    Type

    Blog Post

    Published

    May 4, 2021, 1:15 p.m.

    ...J, Trimmer JS (2019) A toolbox of IgG subclass-switched recombinant monoclonal antibodies for enhanced...

14. Split-BioID: An Improved Method for Studying Protein-Protein Interactions

    Type

    Blog Post

    Published

    May 1, 2018, 1:57 p.m.

    ...methods lack spatiotemporal control or an “on/off switch.” This makes them great tools for providing a global...

15. Plasmids for Endogenous Gene Tagging in Human Cells

    Type

    Blog Post

    Published

    April 6, 2017, 1:02 p.m.

    ...-inducible degrons (AID). Further, the Yamamoto PITCh system plasmids provides an alternative to HDR as...

16. Making CRISPR Plasmids Using Fragmid

    Type

    Blog Post

    Published

    Aug. 29, 2024, 1:15 p.m.

    ...Because of its modular cloning approach, it’s easy to switch out reporters, Cas proteins, guide cassettes, promoters...

17. Story of a SynBio Startup: RevBio's Epiphany (or Lack Thereof)

    Type

    Blog Post

    Published

    June 5, 2014, 3:32 p.m.

    ...store for us. If you’re thinking about making the switch from academia to entrepreneurship, share your thoughts...

18. The Scientific Conference Poster Session: Tips for Success

    Type

    Blog Post

    Published

    Aug. 29, 2019, 12:50 p.m.

    ...poster. Ask the presenter to give the “elevator pitch,” of his/her project – kindly let the presenter ...

19. Academic vs. Industry Postdocs

    Type

    Blog Post

    Published

    Nov. 13, 2018, 1:22 p.m.

    ...after doing a brief academic postdoc. Why did I switch? Well, my long-term goal was to enter industry ...

20. Plasmids 101: Knockout/Knock-In Plasmids

    Type

    Blog Post

    Published

    Dec. 1, 2016, 3:30 p.m.

    ...through Non-Homologous End Joining Learn How the FLEx Switch Can Be Used to Turn Genes On and Off Resources...