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Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 196 results for: p

Showing: 81 - 100 of 196 results

  1. 10 Basic tips for mammalian cell culture

    Type
    Blog Post
    Published
    July 12, 2018, 1:09 p.m.
    ... ER-negative HEK293 cells." PLoS One, 2014. 9(6): p. e98635. PubMed PMID: 24901218. PubMed Central PMCID...2015): 2321-2332. 3. Contamination, Ileana S., John P. S., Basic Methods, Human Stem Cell Manual (Second...

  2. Google Forums Round Up: First Impressions of NgAgo

    Type
    Blog Post
    Published
    Aug. 4, 2016, 2:30 p.m.
    ...Therefore, researchers must co-transfect cells with 5’-P-ssDNA guides and an NgAgo expression plasmid to edit...endonuclease) protein that can form a complex with a 5’-P-ssDNA guide at room temperature. Until that time, ...

  3. Hot Plasmids: Winter 2025

    Type
    Blog Post
    Published
    March 11, 2025, 1:15 p.m.
    ...research   Optimized prime editing in dicots By Emily P. Bentley Prime editing (PE), initially developed in... H., Natale, S., Gomez, L., Taxidis, J., O'Neill, P. S., Villette, V., Bradley, J., Wang, Z., Jiang, D...

  4. Plasmids 101: Modular Cloning Applications and Kits

    Type
    Blog Post
    Published
    May 14, 2024, 1:15 p.m.
    ..., Public Library of Science (PLoS), 18 Feb. 2011, p. e16765. Crossref, https://doi.org/10.1371/journal.pone...5, Public Library of Science (PLoS), 30 May 2018, p. e0197185. Crossref, https://doi.org/10.1371/journal.pone...Communications, vol. 2, no. 2, Elsevier BV, Mar. 2021, p. 100135. Crossref, https://doi.org/10.1016/j.xplc.2020.100135...

  5. Troubleshooting and Optimizing a Western Blot

    Type
    Blog Post
    Published
    Sept. 17, 2024, 1:15 p.m.
    ...membrane stacked behind the p membrane. If your protein runs through the p membrane into the psi membrane...membrane for small proteins (< 15 kDa) and a 0.45 µm (p) membrane for larger ones. Changing your membrane ...membrane, that’s a good indication that the p membrane pores are too large.  Transfer method   Wet transfers...

  7. Plasmids 101: Stringent Regulation of Replication

    Type
    Blog Post
    Published
    June 20, 2024, 1:15 p.m.
    ...Niehaus in December 2015 and was updated by Emily P. Bentley in June 2024.  References and resources ...J. E., Rozgaja, T. A., Gupta, R., Dyson, K., Rao, P., & Leonard, A. C. (2018). Origin recognition is the...

  8. CRISPR 101: Homology Directed Repair

    Type
    Blog Post
    Published
    Jan. 26, 2023, 2:15 p.m.
    ...potential to induce chromosomal aberrations. Pfeiffer P, et al. Mutagenesis (2000). PubMed. Multiple Pathways...Geneticist's practical guide to CRISPR applications. Singh P, et al. Genetics 114.169771; (2014). PubMed. The...

  9. CRISPR 101: Any Base Transversion Editors

    Type
    Blog Post
    Published
    April 1, 2025, 1:15 p.m.
    ..., Zhang, D., Zhang, S., Li, C., Wu, J., Randolph, P. B., Sousa, A. A., Qu, C., Zhu, Y., Guan, Y., Wang... Emily P. Bentley...

  10. A Control for All Seasons

    Type
    Blog Post
    Published
    Oct. 5, 2023, 1:15 p.m.
    ...were run on a western against a protein standard (P) to check for tau molecular weight shifts. A ...PubMed Central PMCID: PMC4212362. Alexander, Steve P. H., et al. “Goals and Practicalities of Immunoblotting...

  11. Finding nucleic acids with SHERLOCK and DETECTR

    Type
    Blog Post
    Published
    April 16, 2020, 1 p.m.
    ...Gootenberg JS, Abudayyeh OO, Lee JW, Essletzbichler P, Dy AJ, Joung J, Verdine V, Donghia N, Daringer NM...Abudayyeh OO, Slaymaker IM, Makarova KS, Essletzbichler P, Volz SE, Joung J, van der Oost J, Regev A, Koonin...

  12. Five Popular Model Organisms, Part 2

    Type
    Blog Post
    Published
    Sept. 29, 2020, 1:15 p.m.
    .... https://doi.org/10.1038/nature02426 Iannaccone, P. M., & Jacob, H. J. (2009). Rats! Disease Models &...Liu, L. S., Zhao, L. Y., Wang, S. H., & Jiang, J. P. (2016). Research proceedings on amphibian model organisms...

  13. Using Phosphoserine to Study Protein Phosphorylation

    Type
    Blog Post
    Published
    June 23, 2016, 2:30 p.m.
    ...incorporated into the full-length protein. Up to 80% p-Ser incorporation into E17TAG GFP has been achieved...incorporation due to differences in stability of the p-Ser incorporated protein as well as context-specific...

  16. Antibodies 101: Multiplex Immunofluorescence

    Type
    Blog Post
    Published
    May 23, 2023, 1:15 p.m.
    ... References and Resources References Andrews, N. P., Boeckman, J. X., Manning, C. F., Nguyen, J. T., ...protocols.io.bs68nhhw Im, K., Mareninov, S., Diaz, M. F. P., & Yong, W. H. (2019). An Introduction to Performing...

  17. CRISPR 101: Engineering the Plant Genome Using CRISPR/Cas9

    Type
    Blog Post
    Published
    Oct. 11, 2016, 2:30 p.m.
    ...doi.org/10.1038/s41477-020-0704-5 Santana I, Wu H, Hu P, Giraldo JP (2020) Targeted delivery of nanomaterials...doi.org/10.1038/s41467-020-15731-w Wang F, Wang C, Liu P, Lei C, Hao W, Gao Y, Liu Y-G, Zhao K (2016) Enhanced...
Showing: 81 - 100 of 196 results