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...This post was updated on Jul 27, 2020. CRISPR, and specifically Cas9 from S. pyogenes (SpCas9), is truly...therefore be the focus of the remainder of this blog post. Perhaps the biggest difference between Cas13a and...tool, as discussed below. Subscribe to CRISPR Blog Posts Applications of Cas13a   Figure 1: Using...to expand the applications of CRISPR. Since this post was originally published, we've also seen the new...and Mary Gearing contributed to the update of this post. References 1. Abudayyeh, Omar O., et al. "C2c2 ...

... Markham's Post on Finding Your Perfect Job After University Browse All of Our Career Posts Resources...This post was contributed by guest blogger Melanie Fox, founder and executive director of Central Indiana...program to write short, funny, picture-filled blog posts about their research? Then we could get everyone...scientific training and wrote about them in a blog post on Born to Science: Life Lessons from Scientific...Indiana Science Outreach and a Postdoc at Indiana University School of Medicine. It’s not always easy to...director of Central Indiana Science Outreach and a Postdoc at Indiana University School of Medicine. She is...

...This post was contributed by guest bloggers Lauren Celano of Propel Careers and Rachel Casseus, Esq. ...of Casseus Law. Disclaimer: The contents of this post are intended to convey general information only ...provide legal advice or opinions. The contents of this post should not be relied upon for legal advice in any... most current legal developments.  Further, this post may contain technical inaccuracies or typographical...in reliance on the information contained in this post and we disclaim all liability in respect to actions...not taken based on any or all the contents of this post.  An attorney should be contacted for advice on ...All of the immigration options discussed in this post benefit from the applicant thinking about the process...

...focus on these tools in this blog post). The second major area deals in post-experiment quality control, a...This post was contributed by guest blogger Cameron MacPherson at the Institut Pasteur CRISPR software...view on current sgRNA design tools The goal of this post is to provide some insight into available CRISPR...Quality control is, however, not a focus of this blog post. I separate sgRNA design tools into database and...tasks and/or by providing features to help with the post-design process such as primer and plasmid design...anywhere else, because I developed them for this post, but they should give you an idea of what to look...Catch up on All the CRISPR Basics Read Other Blog Posts about CRISPR Find validated gRNAs ...

...In January 2016 we first published a blog post titled: Which Cas9 Do I Choose for My CRISPR Experiment...it must be accessible by Cas9. Our previous blog post The PAM Requirement and Expanding CRISPR Beyond ...many applications. At the time of our first blog post on choosing a Cas9, the Feng Zhang Lab had described...resources on the Addgene blog Read our CRISPR 101 blog posts Find more information about Cas proteins Download...

...resources were hosted on the Plantae website blog post written by Ben Schwessinger, which resulted in greater...the ASPB workshop and authored a three-part blog post on the materials that were covered in the workshop...time. They achieved this by administering pre- and post-workshop surveys, recording results, and tracking...Minisymposium on Scientific Reproducibility Read a blog post on Unique and persistent IDs for improved reproducibility...tackle irreproducibility. Benjamin Schwessinger, a postdoc at Berkeley at the time and an eLife ambassador...visibility of these materials, Sonali Roy, a postdoctoral researcher at the Noble Research Institute, ...

...This post was contributed by Scott Findlay, a Postdoctoral Fellow at the University of Alberta. If you...of mutation validation, such as this "CRISPR 101" post. Next-generation sequencing technologies are the...consuming, and minimally informative. In this blog post, we’ll introduce digital PCR as an emerging validation... precision genome editing, the remainder of this post will focus on assays to detect NHEJ. These assays... out of frame! Scott Findlay is currently a Postdoctoral Fellow at the University of Alberta. He is interested...Findlay, S. D., Vincent, K. M., Berman, J. R. & Postovit, L.-M. A Digital PCR-Based Method for Efficient...

...to Novartis for her post-doc in January 2012. She worked for three years as a post-doc in the Target ID...NGS). The library was developed when Carrie was a post-doc in Frank Stegmeier’s lab in Novartis Oncology...Validation Group and says that she decided to do a post-doc at Novartis because she wanted first-hand experience...

...significantly reduced posting on November 20, 2023, and as of today, will stop posting, monitoring, and resharing...

...Addgene blog: Browse our blog posts on science career options Read blog posts on professional development...This post was contributed by Steph Guerra, a AAAS Science and Technology Policy Fellow at the Veterans.... Out of all the career spaces I explored for my post-PhD life, the science policy community is the one...engaging with the #scipol hashtag. Often there will be posts about virtual meet ups and happy hours that you ...itself. You can work to influence Big P policy from a post in non-governmental organizations, universities,...transition out of academia from graduate school or a postdoc. But some are also open to individuals from mid...

...on-brand templates for these posts. Some of recent Meet an Addgenie posts on Instagram! From this...able to express my visions for what my Instagram posts would look like. While working remotely was a different...

...learned along the way in this ongoing series of blog posts. Listen to our podcast interview with Harvard Medical..."Precision and Plasticity in Mentoring" A recent post on the blog “Tenure, She Wrote” is titled, “What... to the other six mentoring questions in future posts. If you have a question about mentoring that you...

...time. Learn more about the Data Hub in this blog post. Alongside our efforts to facilitate the sharing...Vectors 101 eBook Find all of our viral vectors blog posts Learn more about AAVs Resources on the Addgene ...

...like I do, sniffle) ourselves back into our regular post-holiday routines, why not take a little time to ...out every once in a while – just read Melina Fan’s post on lunch. Why should our new blog be any different...

...This post was originally written by Melina Fan and updated Nov 3, 2022 by Susanna Stroik. You’ve designed...genome edits went according to plan. In this blog post we’ll explain how to verify that your cells were...options outlined above aren’t realistic. Check Out Our Post on Sequencing Options for CRISPR Genotyping NGS ...HypaCas9. Read more about Cas9 options in our blog post here. Dealing with low editing frequency Did your...Protocols Find gRNA Design Tools Read other CRISPR blog posts Find CRISPR Plasmids at Addgene  ...

... detectable signals like light or color. In this post, we’ll go over some of the common conjugates you...fluorophores as antibody conjugates in an earlier post, where you can find some additional tips for working...for detecting low abundance targets. Check out our post on Biotinylation to learn more about biotin and ... 3) Endogenous carbohydrates added to antibodies post-translationally. 4) Modifications made to antibodies...

...This guest post was contributed by Johnny Kung, Director of New Initiatives for the Personal Genetics...science education, communication, and policy. In this post, I hope to tell you a little more about how I ended...translating all these into social media and blog posts, lesson plans, or information briefs for policymakers...communication Find all of our science communication posts Extracurricular activities for a strong science ...

...investigate their role in a specific behavior. This blog post will give an introduction to behavioral neuroscience... AAV or lentiviral vectors in our previous blog posts.  Viral vector injection Viral vectors allow a tight...example anxiety or depression. In upcoming blog posts, we’ll discuss some of these behavioral studies ...Addgene blog Read all of our viral vectors blog posts Find tips for preparing AAVs View our Beginner's...

...Plasmids 101 blog posts Read blog posts about using Cre-lox Read our genome engineering blog posts Resources...genetically engineered mice, what can you do? In this post, we’ll cover why and how to cross mice to create...

... Check out the Blog Post on Our Citations Feature Browse More Inside Addgene Posts Additional Resources... you’re ever interested in writing your own blog post about a plasmid or set of plasmids you’ve deposited...