We narrowed to 695 results for: prot

...isolate a protein, there must be a special feature about that protein that no other protein in the expression...affinity tags can make protein purification a cinch. Types of affinity tags, the proteins they work best attached...common tags available. Maltose binding protein Maltose binding protein (MBP) is a 45 kDa tag which can be ...solubility of the tagged protein (Fox and Waugh, 2002). The tag can also be proteolytically cleaved after purification...unlikely to perturb protein function. This makes it amenable to being left on your protein of interest, especially...Wingfield, P. T. (Eds.). (2001). Current Protocols in Protein Science. John Wiley & Sons, Inc. https:/...Maltose-Binding Protein as a Solubility Enhancer. In P. E. Vaillancourt, E. coli Gene Expression Protocols (Vol....

...tag endogenous proteins for studying protein expression, localization, and protein-protein interactions....fluorescently tagged proteins can lead to improper protein localization, protein aggregation, or disruption...used to track protein localization, cell structure, intracellular trafficking, and protein turnover rates...disruption of normal protein function, and ultimately misinterpretation of the protein’s cellular role. One...interest to better understand normal protein function. However, CRISPR protein tagging does have its limitations...donor plasmids for protein tagging, including luciferase (NanoLuc), fluorescent proteins (TagGFP2, TagBFP...for endogenous protein tagging, please visit our website CRISPR/Cas Plasmids - Protein Tagging. Beyond...

...of a responsive protein (e.g. calcium binding protein) fused to two fluorescent proteins. When the responsive...fluorescent proteins to make better reporters and the second couples NLuc to fluorescent proteins to make ...biosensors. NanoLuc®-fluorescent protein fusions for imaging Fluorescent proteins are fantastic imaging tools...penetrative orange fluorescent protein fusion (OgNLuc). Both fluorescent proteins can be excited by Nluc emission... imaging multiple proteins at the same time in live cells using fluorescent proteins (FPs) can be problematic...responsive protein binds to calcium it changes conformation and brings the two fluorescent proteins together... energy from one fluorescent protein to a compatible fluorescent protein. FRET can be measured by exciting...

... the same protocol for generating lentivirus, but you use a different envelope glycoprotein instead of...of the wild-type glycoprotein. The pseudotyped protein is encoded on the envelope plasmid which contains...pseudotype with the SARS-CoV-2 spike protein, scientists encode the spike protein on the envelope plasmid. Image...used envelope protein for pseudotyping is VSV-G, or vesicular stomatitis virus G protein. VSV-G is a trimeric... trimeric protein that binds phosphatidylserine and low-density lipoprotein receptors (LDLRs) on a cell...Other envelope proteins for pseudotyping While VSV-G is the most commonly used envelope protein for pseudotyping...retroviral envelope proteins have been pseudotyped on lentiviral particles. Rabies glycoprotein The rabies glycoprotein...

...MitoTimer proteins are derived from the Timer protein - a mutant of the fluorescent protein dsRed that...groups to monitor protein and cell ageing, as the spectrum shift occurs during Timer protein life due to a... synthesized (green) protein and organelles with older (yellow to orange) protein. Image courtesy of Roberta...1852-61. A quicker method for protein inactivation Your protein of interest does x, but it also participates...your endogenous protein of interest and co-expressing its replacement recombinant protein with a FKBP domain...outer membrane protein with a FRB domain (MitoTRAP). Rapamycin binds your recombinant protein (via FKBP) ... fused to proteins of interest for EM imaging, or targeted to subcellular regions or protein complexes...

... the pocket of a folded protein, or blocked by something bound to the protein, the antibody can't bind... of the proteins to the microtiter walls for ELISAs and a more accurate separation of proteins by mass...working with native proteins instead of denatured ones - well, don’t relax just yet. Proteins often change shapes...looking for proteins that are interacting with one another, or if you’re looking for a protein of interest...you want to capture the protein bound to anything? Is it a transmembrane protein, or is it know to translocate...browse the collection, you'll likely find some proteins of interest for which you have several antibodies... are there so many options for a single target protein? Epitope Availability The answer is simple: epitope...

...during protein translation (Ermolenko et al., 2007). All that is required is that the proteins/nucleic...fluorescent donor to your protein is to hardwire the molecule directly into the protein’s peptide sequence. Thus...you are inserting about 20-25 kDa of protein mass into your protein and so structural changes due to this...target protein, but even these modifications can affect the structure or function of certain proteins. Rather...your protein of interest: you just don’t know it yet! The poly-histidine tag often used for protein purification...NTA-sepharose beads used for protein purification: the NTA binds to the His tag on your protein and the coupled Cy... the same protein and a negative control experiment conducted on donor-only labeled protein that is incubated...

...Fluorescent Protein (GFP) and other fluorescent proteins revolutionized live-cell protein detection, their...clinically-relevant proteins are also the hardest to study. One common strategy is protein overexpression ...capture true protein behavior. Traditional epitope knock-ins maintain the native protein context, but ... or unique surface epitopes for many proteins, but some proteins do not have such epitopes. These classical...tracking proteomic cargo flow via EVs. Find ADAR-HiBiT Fusion Vector at Addgene! Watching protein degradation...Studying proteins in their natural context is one of the biggest challenges in biology. From tumor suppressors...only snapshots rather than continuous views of protein activity. Together, these limitations create challenges...

...combinations of protein tags (His, Avi, and GFP) as well as protease sites to facilitate protein purification...plasmids contain peptides to facilitate protein folding and/or protease sites to remove the epitope tags. Different...the system Easing the Protein Purification Process with pCri Cell-free protein expression kit Cell-free.... The Cell-Free Protein Expression Kit has been used to produced recombinant proteins for in-vitro assays...binding proteins in as little as 3 days. Abil et al., 2014 show that you can fuse custom PUF proteins to a...Cloning Kit BIOFAB Plasmid Set pCri System Cell Free Protein Expression Kit FREQ Seq PUF Assembly Kit Below...of interest into both sequencing (pInitial) and protein production (a variety of pExpression vectors) plasmids...

...favorite protein in your hand? At 3D Molecular Designs, we create physical models of proteins using 3D...images of proteins. But they are still flat. To truly appreciate the 3D structure of these proteins, it is...physical models of protein structure? We have been creating physical models of proteins for researchers ...While a physical model of a protein might represent one aspect of the protein very well, it will almost ... of all proteins in general, and the amazing conformational flexibility that the Cas9 protein must have...Molecular Designs. Have you ever held your favorite protein in the palm of our hand? Well, actually – have ...to hold one. Why do we need physical models of protein structure? Structural biologists have always struggled...

...be isolated from the serum. Protein A/G purification Protein A or Protein G purification is one common...or not, while any other proteins pass through. However, protein A and protein G each have different affinities... containing the target protein, and any antibody recognizing the target protein remains bound to the column...immobilized serum protein from other species. Antibodies that don’t cross-react with the serum proteins pass through...target protein due to their ability to recognize many different epitopes of the target protein. While ... detect a protein in low abundance or whose conformational state may change. If your protein might become...mixture of many antibodies that recognize the same protein. In the immune system, antibodies are produced ...

...distinguish between proteins bound by the antibody vs proteins bound to the target protein, so it’s important...your antibody is not binding to proteins outside of your target protein. Show suitability for an intended...of a protein of interest. Conveniently, you found a cell line in which the gene for your protein has been...express your protein of interest endogenously and transfect them with a plasmid encoding your protein of interest...expected target protein in a Western blot. You acquire a plasmid encoding your protein of interest, known... the majority of the protein in the sample was identified as your target protein, allowing you to proceed...methods may include analysis of transcriptomic or proteomic data from several different tissues or cell types...

... Abundant (LEA) proteins, small proteins that naturally adsorb to the AWI, can protect cryo-EM samples...enrichment and structure determination LEA proteins protect samples during cryo-EM grid freezing Engineered... of proteins captured on magnetic beads (Arimura et al., 2024). In MagIC-cryo-EM, target proteins (expressed...doi.org/10.1101/2024.01.21.576499.    LEA proteins protect fragile samples during cryo-EM grid plunge...complexes may dissociate  (right). B) Protein samples protected from AWI by helical LEAs. C-E) cryo-EM.... J. (2024). Small LEA proteins as an effective air-water interface protectant for fragile samples during... DNA-targeting proteins, so the team created versions using dCas9, zinc finger proteins, and transcription...

...Biotin protein ligase (BPL) is responsible for this highly specific post-translational protein modification...the lab are protein purification and immunoprecipitation experiments. Biotinylated proteins or complexes...terminus of the protein. BirA has been shown to effectively biotinylate AviTag expressing proteins in bacteria...fusion proteins Learn how biotin is used in Split-BioID: An improved method for studying protein-protein...biology for an array of different techniques and protocols. In this post we will discuss the natural role...about in vivo biotinylation of bacterial fusion proteins Biotin is an essential vitamin for life Although... general process by which biotin is added to a protein or macromolecule. Biotin can be added enzymatically...

... Which Fluorescent Protein Should I Use? However, fusion proteins tag your protein of interest with a ...target protein composition. It is used for a variety of applications such as measuring target protein expression...produces a greater SSC.  Protein expression, measured using fluorescent protein fusions In addition to ...-stained protein will emit a stronger photon than a cell expressing low levels of the protein. The electrical...on the N- or C-terminus of the protein. One downside of fusion proteins is that the tag can alter the ...structure of the protein. In some cases this can lead to changes or a complete loss of protein function. In...the challenges of fluorescent protein fusions by binding to the protein in its native state.  Antibodies-based...

...amplification protocol developed for each individual pooled library; generalized amplification protocols tend ...reference protocols There are many decisions to make when developing your amplification protocol! We recommend...one-size-fits-all” protocol! Each library is different, and you should always experimentally test the protocol you design...surprised to learn that we ask for an amplification protocol with your deposit. This is because repeated amplifications... we’ll discuss how to develop an amplification protocol for your pooled library deposit. When a requesting...consider when creating a pooled library amplification protocol. Created with BioRender.com.   The most important...efficiency you need to achieve. Most pooled library protocols aim for somewhere between 30x and 200x coverage...

...)est fluorescent protein Fluorescent Proteins 101: A History of Fluorescent Proteins ...attached to self-labeling proteins. Some common examples of these self-labeling proteins are SNAP-tag® and HaloTag...to using these self-labeling proteins over traditional fluorescent protein tags, such as high specificity...many self-labeling protein fusions as well as empty backbones to clone your own protein of interest. Fluorescence.... FITC binds to an amine on a protein, enabling the labeling of protein substrates/peptides and antibodies... as conjugation with proteins, should be carefully managed. Proper dye-to-protein ratios, or the degree...cleavage of FITC-conjugated protein ligands is unaffected by additional protein from cells under certain ...

...insight into protein:DNA interactions. ChIP works by using antibodies to capture protein:DNA complexes with...to your protein(s) of interest. Once the complex is captured, it is treated so that the protein and DNA... antibody on crosslinked proteins. In the case of histone modifying proteins, which bind quite strongly...with RNAse and Proteinase K to purify the DNA and then determine concentration.  Protein Capture After ...that ChIP uses native proteins (so antibodies validated only for denatured proteins may not be a good choice...specifically select for proteins of interest (4) isolated your antibody:protein:DNA complexes and finally... to firmly attach the proteins to the DNA, depending on how tightly the proteins bind natively. But the...

...permeabilization protocol, but very poorly in a methanol fixation-permeabilization protocol. Although the... the optimal knockdown of the protein target will depend on the protein turnover rate. If you need a more...suppress the expression of many related proteins, not just your protein of interest, which can affect your ... levels of the target protein. It may only be able to detect the target protein at the high levels caused...Additionally, their performance depends on the specific protocol used and the abundance of the epitope in the sample...can detect the antigen of interest in the given protocol. This requires use of one of the five pillars ... of which are commercially available. Various proteomic and transcriptomic datasets can be used to select...

...C terminus of a protein may disrupt the biological function of some proteins. Protein folding issues, ...of detection of a mutant protein vs. endogenous WT levels of the same protein when both are expressed ...to a protein’s termini to increase detection. The tag can also be removed by treating the protein with...validate tagged protein functionality (Arribere, et al). In rare cases, tags can also affect protein localization...for your application, specifically detects your protein, is species compatible, and doesn’t come with a...peptides introduced at the N or C terminus of a protein that are bound by antibodies for the purpose of...of purification or detection of the tagged protein. These peptides consist of as little as 8 or as many...