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Showing: 81 - 100 of 272 results

  1. Rabies and Neuronal Tracing

    Type
    Blog Post
    Published
    May 29, 2018, 1:51 p.m.
    ...into viral genome, so viral progeny will not necessarily express EnvA unless it is supplied in trans. ...phenomenon has shown that the RABV G protein is necessary and sufficient for retrograde transmission (Etessami...

  2. Viral Vectors 101: Calcium Sensors

    Type
    Blog Post
    Published
    Aug. 10, 2023, 1 p.m.
    ...calcium binding sites per sensor (to decrease unnecessary calcium buffering and to increase sensitivity...great FRET sensor option if you have the setup necessary for readout.   We’ve got a live one ...

  3. Plasmids 101: The Wide World of Natural Plasmids

    Type
    Blog Post
    Published
    Oct. 8, 2024, 1:15 p.m.
    ... toxins like colicin typically also carry the necessary resistance genes to help cells survive their own...cell, but this functional organization doesn’t necessarily reflect genetic relationships or plasmid incompatibility...

  4. Plasmids 101: In-house NGS Plasmid Quality Control Now at Addgene

    Type
    Blog Post
    Published
    April 6, 2021, 12:30 p.m.
    ...sequence like a known backbone sequence. We’re not necessarily expecting a perfect match - we will often find...insert of the library. This PCR step also adds the necessary adapter sequences for Illumina sequencing as well...

  5. Savvy Advocates Needed to Navigate a Scientific Enterprise in Flux

    Type
    Blog Post
    Published
    Sept. 13, 2016, 2:30 p.m.
    ...way it is funded and to learn tangible skills necessary for affecting change. On Friday, the symposium...designed to explore areas where further data is necessary to determine the best solutions. The event also...

  6. 10 Basic tips for mammalian cell culture

    Type
    Blog Post
    Published
    July 12, 2018, 1:09 p.m.
    ... have the power to take the added precautions necessary to keep your cells from becoming contaminated....you’re wearing before you start. Also, avoid unnecessary talking because talking generates microbe-laden...

  7. Viral Vectors 101: Voltage Indicators

    Type
    Blog Post
    Published
    Jan. 30, 2024, 2:15 p.m.
    ...with these sensors, but further optimization is necessary for these to be as effective at the single cell...perform poorly in two-photon conditions, which are necessary conditions for penetrating deeper tissues depths...

  8. Transgenic Organisms, Cas9 Gene Drives, and Appropriate Safeguards

    Type
    Blog Post
    Published
    May 22, 2015, 4:58 p.m.
    ...Because the consequences of such a mistake would necessarily extend far beyond the laboratory and seriously...inherently collective technology: because they necessarily alter the shared environment, they must never...

  9. Summer SciComm Series: Modes of Communication

    Type
    Blog Post
    Published
    July 1, 2022, 1:15 p.m.
    ...content.  What are you interested in? Math, sarcasm, romance, and spacecraft! Image courtesy of xkcd.com... of a learning curve it will be to get to the necessary level  of proficiency.  What kind of content are...

  10. Better Dyeing Through Chemistry & Small Molecule Fluorophores

    Type
    Blog Post
    Published
    Sept. 8, 2016, 2:30 p.m.
    ...showed that the selective interaction between a bisarsenical dye ligand (e.g., FlAsH, ReAsH) and a short ...Some chemical labeling strategies such as the bisarsenical dye (Figure 1a) and FAP systems (Figure 1e) ...

  11. Plasmids 101: Gateway Cloning

    Type
    Blog Post
    Published
    Jan. 12, 2017, 3:30 p.m.
    ...an expression clone with all of the components necessary for gene expression.     The LR Reaction ...all kind of organisms from mammals to E. coli. Glossary of Gateway cloning vectors Vector type  Vector...

  12. Twenty Years of Sharing: Addgene's Viral Vector Service

    Type
    Blog Post
    Published
    July 30, 2024, 1:15 p.m.
    ...As part of Addgene’s 20th anniversary celebration, we’ve been finding and sharing stories unique to our...following our blog, you may recall our first 20th anniversary story on CRISPR, where we dove fairly deeply ...

  13. CRISPR 101: Any Base Transversion Editors

    Type
    Blog Post
    Published
    April 1, 2025, 1:15 p.m.
    ...post has a lot of acronyms, so we've included a glossary at the end of this post! Figure 1: Base...come up with next! References and Resources Glossary Here’s a quick refresher if you ever lose track...

  14. AAV Titers: Where Do They Come From And What Do They Mean?

    Type
    Blog Post
    Published
    Nov. 15, 2017, 3 p.m.
    ...theoretical maximum value, physical titers don’t necessarily give any indication of the infectious titer. ...and convenient. However, these methods are not necessarily precise or accurate for quantifying AAV because...

  15. Transferable Skills Guide: Career Planning Resources

    Type
    Blog Post
    Published
    Nov. 14, 2017, 2 p.m.
    ..., though – someone accepting your CV doesn’t necessarily mean that you are a future employee of the company...might even be a colleague. A mentor does not necessarily need to be someone a lot older than you are; ...

  16. Data Freedom: The Expansion of Data Sharing in Research Publications

    Type
    Blog Post
    Published
    Aug. 5, 2014, 6:51 p.m.
    ...organization left open the question of how much data was necessary and reasonable. PLOS has since clarified their..., itself, has not finalized its definition of necessary data requirements and likely will not do so (calling...

  17. Plasmids 101: The Promoter Region – Let's Go!

    Type
    Blog Post
    Published
    April 3, 2014, 8:05 p.m.
    ...resistance gene and the origin of replication are necessary to replicate plasmids and make sure the cells ... it. Here, we'll cover the promoter, which is necessary for getting the plasmid express our gene of interest...

  19. Fluorescent Proteins 101: When GFP lets you down

    Type
    Blog Post
    Published
    Aug. 23, 2018, 12:05 p.m.
    ...brightness (Tsien, 1998). Another mutation (A206K) is necessary to generate the strictly monomeric EGFP variant...Hink et al., 2000). The complete beta-barrel is necessary for its fluorescence and therefore GFP cannot ...
Showing: 81 - 100 of 272 results