...behind Gram’s crystal violet stain and the ion interactions that cause the stain retention in gram positive...When the negatively charged iodine is added, it interacts with the CV+, creating a complex within the peptidoglycan...trapping the large dye complex created by the interaction of CV+ and I- in the thick multilayered membrane...can be used in medical settings to determine the general type of bacteria present in an infection. This ...

...distances. For instance, how much force can be generated by conformational changes in proteins? How can...can be addressed using R bodies: simple, force-generating protein machines that can extend and retract ... might be able to endow cells with new ways to interact mechanically with their environment.   Jessica...

...to interview, part of the interview process was several short talks by current faculty members and it just...it looks good, that’s a high that can last for several days.” Working with CRISPR Recently Doench has...since” and that he’s “very much in line with the overall mission of making science easier and more transparent...s] outreach scientists do to comb through the literature, to go and talk to the people who actually have...

...content - the useful information on scientific literature, technical skills, and experiences from thousands... Nearly everything we write entails a primary literature search to collect up to date information on the...review process led to quick dives back into the literature to make sure absolutely everything was correct... typos, and hit publish. Making content is an iterative process. We are constantly reviewing the resources...

...yeast, are temperature sensitive screens. These involve raising or lowering the culture temperature to exacerbate...drug-containing media in which only cells which can tolerate the drug survive and everything else dies off....though. A selection can take the form of varied temperature, nutrient availability, drugs, and much more....

...study microbiome interactions with Alzheimer's, I found others using CRISPR to generate new knockout mice...within the biological sciences, there’s plenty of interaction between neuroscience and other fields. For instance...us produce new educational content on the blog. Overall, there are many exciting things going on in the...

...expression requirements (Figure 1), but Cas12a has generally been underutilized in genetic screens because ...technique, researchers identified a synthetic lethal interaction between MARCH5 and WSB2 genes using Cas12a’s ...similarly to Cas9 screens in a compacted library. Overall, Cas12a’s ability to target multiple genes within...possibilities in both cell engineering and gene therapy.  Xiaoshu X, et al. Molecular Cell. 2021. https...

...post, we’ll touch on antibody storage, buffer considerations, and give just the lightest of nods towards...smaller than 10uL. You can leave them at room temperature when you’re working with them, but avoid freeze-thaw...okay, Blugene!    Buffers  You know what temperature to store your antibodies at, but it also matters...commercial kits.  However, antibodies conjugated to horseradish protein (HRP), primarily used in Western blots...

...interest by PCR generates mutated plasmids with no methylation (there are no methyltransferases in the PCR ...DNA. The other component of these systems, methyltransferases, methylate DNA at particular sequences to...restriction modification systems containing methyltransferases that add methyl groups to specific DNA sequences...

...to do a massive RNA-seq analysis. I had never interacted with a computer at the command line, and so was...keep track of the files that I was continuously generating.  I quickly found that there is no one way to...physical lab notebook, along with any figures generated from the analyses: Goal: Before you begin, briefly...control your commonly used scripts: If you edit a general purpose script for a single use, save the script...