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We narrowed to 729 results for: NSI

Showing: 491 - 500 of 729 results

  1. Mobile-CRISPRi: Bringing CRISPRi to Diverse Bacteria

    Type
    Blog Post
    Published
    April 4, 2019, 12:53 p.m.
    ..., cultures treated with a folA gRNA were more sensitive to trimethoprim when compared to control gRNA ...for trimethoprim, there would be no changes in sensitivity. Pooled knockdowns are also possible with Mobile-CRISPRi...

  2. Of Myc and Men

    Type
    Blog Post
    Published
    Jan. 19, 2023, 2:15 p.m.
    ...epitope, a phenomenon known as sequence context sensitivity (Schüchner, et al. 2020). In their study, some... newer Myc-tag antibodies appeared to be less sensitive to sequence context.    So, do Schüchner, et al...

  3. Degrading DNA with Cascade-Cas3

    Type
    Blog Post
    Published
    Feb. 11, 2025, 2:15 p.m.
    ... increase efficiency of these systems, though consistent expression can still be a challenge. A multitasking...powerful than it is. The nuclease domain of Cas3 is considered weak, especially compared to the power of Cas9...

  4. Antibodies 101: Flow Compensation

    Type
    Blog Post
    Published
    May 16, 2024, 1:15 p.m.
    ...which way your population needs to be shifted, consider your perpendicular lines. In this case, the population...applying very large compensation values (100+), consider what two markers your fluorescent markers are ...

  5. 10 Basic tips for mammalian cell culture

    Type
    Blog Post
    Published
    July 12, 2018, 1:09 p.m.
    ...normally lies around 7.35-7.4 so mammalian cells are sensitive to pH. Anything between orange and light orange...Additional passages will allow your cells to achieve a consistent growth rate. For every minute spent organizing...

  6. Truncated gRNAs for Regulating Gene Expression

    Type
    Blog Post
    Published
    Jan. 10, 2017, 3:37 p.m.
    ...nuclease-null—in order to effect different perturbations. Consider, for instance, the challenge of attempting to ...alterations can be exploited to quickly and inexpensively generate a potent Cas9-based transcriptional...

  7. 3D Printing Meets CRISPR Cas9

    Type
    Blog Post
    Published
    Oct. 17, 2017, 12:55 p.m.
    ... 3-D path of the protein’s backbone in three-dimensional space, but their flexible and jiggly nature suggested...structure into experimentally determined electron density, have been known to say something silly like “See...

  8. Plasmids 101: Knockout/Knock-In Plasmids

    Type
    Blog Post
    Published
    Dec. 1, 2016, 3:30 p.m.
    ...can survive positive selection, but it will be sensitive to negative selection. Remove the positive selection...creating knockout animals or cell lines, please consider sharing them by depositing with Addgene! Figures...

  9. Tips for a 1st time AAV user (by a Rookie AAV user)

    Type
    Blog Post
    Published
    Oct. 23, 2018, 11:49 a.m.
    ...primarily found in the culture media, you should consider performing PEG precipitation. Instead of sonicating...solutions are carefully layered and then the viral suspension generated during AAV production is overlaid. ...

  10. Addgene's Cancer Collection Pages Connect the Research Community

    Type
    Blog Post
    Published
    Sept. 6, 2016, 2:30 p.m.
    ...suppressors, and their protein products. Proto-oncogenes consist of a group of genes/proteins that, when expressed...Ian A., Paul D. Lewis, and Carla Mattos. "A comprehensive survey of Ras mutations in cancer." Cancer research...
Showing: 491 - 500 of 729 results