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We narrowed to 837 results for: Eras

Showing: 501 - 510 of 837 results

  1. How Addgene Creates Content

    Type
    Blog Post
    Published
    Nov. 17, 2022, 2:15 p.m.
    ...content - the useful information on scientific literature, technical skills, and experiences from thousands... Nearly everything we write entails a primary literature search to collect up to date information on the...review process led to quick dives back into the literature to make sure absolutely everything was correct... typos, and hit publish. Making content is an iterative process. We are constantly reviewing the resources...

  2. Plasmids 101: Screens vs. Selections

    Type
    Blog Post
    Published
    Nov. 10, 2022, 2:15 p.m.
    ...yeast, are temperature sensitive screens. These involve raising or lowering the culture temperature to exacerbate...drug-containing media in which only cells which can tolerate the drug survive and everything else dies off....though. A selection can take the form of varied temperature, nutrient availability, drugs, and much more....

  3. Hot Plasmids - February 2022

    Type
    Blog Post
    Published
    Feb. 22, 2022, 2:15 p.m.
    ...expression requirements (Figure 1), but Cas12a has generally been underutilized in genetic screens because ...technique, researchers identified a synthetic lethal interaction between MARCH5 and WSB2 genes using Cas12a’s ...similarly to Cas9 screens in a compacted library. Overall, Cas12a’s ability to target multiple genes within...possibilities in both cell engineering and gene therapy.  Xiaoshu X, et al. Molecular Cell. 2021. https...

  4. Antibodies 101: Buffers, Storage, and Conjugates

    Type
    Blog Post
    Published
    Feb. 1, 2022, 2:15 p.m.
    ...post, we’ll touch on antibody storage, buffer considerations, and give just the lightest of nods towards...smaller than 10uL. You can leave them at room temperature when you’re working with them, but avoid freeze-thaw...okay, Blugene!    Buffers  You know what temperature to store your antibodies at, but it also matters...commercial kits.  However, antibodies conjugated to horseradish protein (HRP), primarily used in Western blots...

  5. A Novice's Perspective on Neuroscience - SfN2017

    Type
    Blog Post
    Published
    Dec. 15, 2017, 2:07 p.m.
    ...study microbiome interactions with Alzheimer's, I found others using CRISPR to generate new knockout mice...within the biological sciences, there’s plenty of interaction between neuroscience and other fields. For instance...us produce new educational content on the blog. Overall, there are many exciting things going on in the...

  6. Plasmids 101: Methylation and Restriction Enzymes

    Type
    Blog Post
    Published
    June 30, 2016, 2:30 p.m.
    ...interest by PCR generates mutated plasmids with no methylation (there are no methyltransferases in the PCR ...DNA. The other component of these systems, methyltransferases, methylate DNA at particular sequences to...restriction modification systems containing methyltransferases that add methyl groups to specific DNA sequences...

  7. How to Keep a Lab Notebook for Bioinformatic Analyses

    Type
    Blog Post
    Published
    June 2, 2016, 2:30 p.m.
    ...to do a massive RNA-seq analysis. I had never interacted with a computer at the command line, and so was...keep track of the files that I was continuously generating.  I quickly found that there is no one way to...physical lab notebook, along with any figures generated from the analyses: Goal: Before you begin, briefly...control your commonly used scripts: If you edit a general purpose script for a single use, save the script...

  8. Protein Tagging with CRISPR/Cas9: A Conversation with Mendenhall and Myers

    Type
    Blog Post
    Published
    July 28, 2015, 2:30 p.m.
    ... in the human genome. The pair has focused considerable attention on transcription factors, starting...Myers: Our labs work really closely together on several projects and this is one related to gene expression...There’s also a question about how specific those interactions are. This is solved in this CRISPR system with...Gene Fragment from Integrated DNA technologies. Generally speaking, anyone using CRISPR/Cas9 in the lab ...

  9. Pooled CRISPR Libraries Offer Genome-Wide Control for Large-Scale Functional Screens

    Type
    Blog Post
    Published
    Feb. 24, 2015, 7:50 p.m.
    ...Broad Institute's Silvana Konermann, the first generation of CRISPR-Cas9 plasmids were not designed with... off,” he said. “Some are strong and some more moderate; there is an allelic series where the degree to... use in various cell types and in live mice. Therapeutic uses in humans are also on the table. “In the...with the libraries will require access to next generation sequencing technology. These libraries are ...

  10. 3 Challenges in Plant Synthetic Biology

    Type
    Blog Post
    Published
    July 22, 2014, 5:46 p.m.
    ...technology and giving consumers control over their interaction with it, we hope to inspire and open a new conversation...synthetic biology In addition to public resistance, an overall limited understanding of plant biology is a significant... genetic pieces developed over the years were generated by large agricultural companies and carry associated...biotechnology is full of potential, but there are several hazards to navigate when mapping out a new project...
Showing: 501 - 510 of 837 results