We narrowed to 842 results for: Eras

...expression, localization, and protein-protein interactions. Beyond these common applications, researchers...when exposed to ultraviolet light. To do so, she generated fusion proteins in which Dronpa domains flanked...Proteins, or FLiPs.  Xin later optimized these first generation FLiPs by mutating Dronpa to lower its aggregation... upon exposure to ultraviolet light. Previous iterations of FLiPs only used N- and C-terminal dronpa fusions...

...of sample preparation for high-throughput next generation (NGS) sequencing have prevented laboratories ...each well of a 96-well plate. Then, samples from several plates are pooled together to create a single library...harder and harder to obtain balanced and sufficient coverage of all samples. The root of the normalization ...important for getting adequate sequencing depth and coverage in order to, for example, assemble hundreds of...

...depending on the primer sequence and the NEB polymerase used. Overall, while much of the information in the ...helpful, but does make it easy to skim the manual. Overall, it’s a great tool for someone starting cell culture... it had a codon list for each amino acid, but, overall AMINO can be beneficial for new researchers or ...

...distances. For instance, how much force can be generated by conformational changes in proteins? How can...can be addressed using R bodies: simple, force-generating protein machines that can extend and retract ... might be able to endow cells with new ways to interact mechanically with their environment.   Jessica...

...to interview, part of the interview process was several short talks by current faculty members and it just...it looks good, that’s a high that can last for several days.” Working with CRISPR Recently Doench has...since” and that he’s “very much in line with the overall mission of making science easier and more transparent...s] outreach scientists do to comb through the literature, to go and talk to the people who actually have...

...study microbiome interactions with Alzheimer's, I found others using CRISPR to generate new knockout mice...within the biological sciences, there’s plenty of interaction between neuroscience and other fields. For instance...us produce new educational content on the blog. Overall, there are many exciting things going on in the...

...expression requirements (Figure 1), but Cas12a has generally been underutilized in genetic screens because ...technique, researchers identified a synthetic lethal interaction between MARCH5 and WSB2 genes using Cas12a’s ...similarly to Cas9 screens in a compacted library. Overall, Cas12a’s ability to target multiple genes within...possibilities in both cell engineering and gene therapy.  Xiaoshu X, et al. Molecular Cell. 2021. https...

...post, we’ll touch on antibody storage, buffer considerations, and give just the lightest of nods towards...smaller than 10uL. You can leave them at room temperature when you’re working with them, but avoid freeze-thaw...okay, Blugene!    Buffers  You know what temperature to store your antibodies at, but it also matters...commercial kits.  However, antibodies conjugated to horseradish protein (HRP), primarily used in Western blots...

...content - the useful information on scientific literature, technical skills, and experiences from thousands... Nearly everything we write entails a primary literature search to collect up to date information on the...review process led to quick dives back into the literature to make sure absolutely everything was correct... typos, and hit publish. Making content is an iterative process. We are constantly reviewing the resources...

...yeast, are temperature sensitive screens. These involve raising or lowering the culture temperature to exacerbate...drug-containing media in which only cells which can tolerate the drug survive and everything else dies off....though. A selection can take the form of varied temperature, nutrient availability, drugs, and much more....