... in the human genome. The pair has focused considerable attention on transcription factors, starting...Myers: Our labs work really closely together on several projects and this is one related to gene expression...There’s also a question about how specific those interactions are. This is solved in this CRISPR system with...Gene Fragment from Integrated DNA technologies. Generally speaking, anyone using CRISPR/Cas9 in the lab ...

...Broad Institute's Silvana Konermann, the first generation of CRISPR-Cas9 plasmids were not designed with... off,” he said. “Some are strong and some more moderate; there is an allelic series where the degree to... use in various cell types and in live mice. Therapeutic uses in humans are also on the table. “In the...with the libraries will require access to next generation sequencing technology. These libraries are ...

...technology and giving consumers control over their interaction with it, we hope to inspire and open a new conversation...synthetic biology In addition to public resistance, an overall limited understanding of plant biology is a significant... genetic pieces developed over the years were generated by large agricultural companies and carry associated...biotechnology is full of potential, but there are several hazards to navigate when mapping out a new project...

...cultured with isotope-labeled amino acids for several hours to several days, followed by a short period of growth...via the secretory pathway, and then cleaved to generate an active form of the protein. To monitor autophagy...Pho8Δ60 in the vacuole, where it is cleaved to generate an active enzyme. Pho8 phosphatase activity or...

...phenotypes. Factors such as the growth medium, growth temperature, and growth rate can all impact plasmid copy ... as the incoherent feedforward loop (iFFL). In general, iFFLs use an input signal that both positively...repression in E. coli. In addition, TALEs bind non-cooperatively to the promoter region, which is predicted to... pathway will ideally be optimized to promote generation of the desired product while avoiding the accumulation...

... would do if she wasn’t at the bench.  “I was literally sitting in my office one day, which I shared with...NEI), where she wrote about NEI research for a general audience. She then got a full time position with..., however, recommend using Twitter as a way to leverage unpaid work for paid work: “If you use it right...helped because there was so much work through the federal government. All the Health and Human Services (...

...there’s less malignancy because there’s less proliferation. However, the lack of caveolin 1 in breast cancer...tumor, proving once again that a tumor is always interacting with its surroundings and being shaped by them...growth mediators and improving cancer cells proliferation.  How do microenvironments affect tumor spread...regulates transcription. Without TKA1, cells proliferate and cannot undergo apoptosis, thus multiplying...

...mercaptopyruvate sulfurtransferase (MPST) which catalyzes the desulfuration of 3-mercaptopyruvate and generates an ...in the lab. He has recently developed a third-generation intensiometric glutamate sensing fluorescent ...possible due to the collaborative and highly interactive environment that Janelia offers,” says Aggarwal.In...

...alternative reagents are created in the lab, instead of generated in vivo, they have higher reproducibility and ... Fig. 1: A comparison of an antibody (left) to several alternative affinity reagents.    Types of ...small enough to be produced in bacteria and are generally cheaper to make than antibodies. Minibodies are...instead of the antibody constant region. They are generated using combinatorial libraries, and high specificity...

...antibodies work?  Secondary antibodies are antibodies generated against antibody isotypes from a specific species...classes, it is possible to get secondary antibodies generated against isotype subclasses, such as mouse IgG2...interest - the secondary antibody needs careful consideration before selection. If all the primary antibodies...my goat anti-rabbit?!”*  *a secondary antibody generated in goat against rabbit immunoglobulins. References...