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We narrowed to 928 results for: Gene

Showing: 501 - 510 of 928 results

  1. Seeing Red: Simple GFP Photoconversion

    Type
    Blog Post
    Published
    Aug. 4, 2015, 2:30 p.m.
    ... GFP-containing organism,” says Hanson, “using a gene encoding one of the unexpectedly photoconvertible... Find plasmids from this publication at Addgene.   Addgene’s Fluorescent Protein Plasmids and Resources...multiple important technologies, including FRET and optogenetics. Even though GFP has been so thoroughly characterized... GFP is great news for microscopy enthusiasts! Addgene has empty backbones containing GFP variants used...PubMed. Find plasmids from this publication at Addgene. Subach OM, Patterson GH, Ting LM, Wang Y, Condeelis...

  2. CRISPR Protocol for Genomic Deletions in Mammalian Cell Lines [Video]

    Type
    Blog Post
    Published
    Feb. 18, 2015, 3:09 p.m.
    ...approach is to target two exonic sites within a single gene using paired DSBs. This method allows for deletion...Medical School. Addgene is proud to present a video reprint of the CRISPR article "Generation of Genomic Deletions...within the deletion boundaries may be produced to generate clonal allelic series. Single DSBs resolved by...biologists can imagine, create, and examine novel genetic configurations. Watch the following video to learn...the full protocol from the JOVE article here on Addgene's website and find more CRISPR resources.    Thank... Medical School. His lab studies the molecular genetics of blood cell development and disease. Learn more...al. An erythroid enhancer of BCL11A subject to genetic variation determines fetal hemoglobin level. Science...

  3. Celebrating Outstanding CRISPR/Cas9 Achievements at the Dr. Paul Janssen Award Dinner

    Type
    Blog Post
    Published
    Sept. 15, 2014, 6:05 p.m.
    ...they have been able to use CRISPR/Cas9 to generate mice with genes knocked out in the insulin signaling cascades...and Dr. Emmanuelle Charpentier. I was joined by Addgene Scientist Matt Ferenc (one of our resident CRISPR... to a year or greater with older technologies. Addgene is proud to be helping get CRISPR reagents out ...  New to CRISPR/Cas9 Technology? Learn More: Addgene's CRISPR/Cas9 Guide History of CRISPR Cas - A tale...

  4. Plasmids 101: Dimers and Multimers

    Type
    Blog Post
    Published
    Jan. 10, 2023, 2:15 p.m.
    ...CGE), which has been used in plasmid vaccine and gene therapy development to examine plasmid topologies...plasmid DNA concatemers in mammalian cells. J Gene Med. 2009 May;11(5):444-53. doi: 10.1002/jgm.1310. PMID: ...resources on Addgene.org  Addgene's Plasmids 101 eBookAddgene's Molecular Biology ReferenceAddgene's Restriction...length of the monomer (~11.6 kb). Image generated by SnapGene software.  Thanks to increasing affordability...RA, James AA, Kolodner R. recA-independent general genetic recombination of plasmids. Nature. 1981 Nov... performing restriction digests on plasmids at Addgene, we sometimes observe something odd in our uncut... the short-read Illumina MiSeq sequencing that Addgene uses.) However, when some researchers received ...

  5. Antibodies 101: Affinity Tags

    Type
    Blog Post
    Published
    Sept. 19, 2023, 1 p.m.
    ...Solubility Enhancer. In P. E. Vaillancourt, E. coli Gene Expression Protocols (Vol. 205, pp. 99–118). Humana...-59259-301-1:99 Resources on Addgene.org Addgene antibodies Addgene’s antibody guide Antibody protocols...having solubility issues!). Need a GST antibody? Addgene has you covered! His His tags, often called polyhistidine...Purification of His fusion proteins from E. coli is generally more straightforward and of a higher purity than...and other qualities of each tag class, they are generally best suited for their primary application. Ready...protocols  Resources on the Addgene blog Plasmids 101: Protein tags Antibodies 101: Epitope tags To Each...

  6. What the HEK?

    Type
    Blog Post
    Published
    April 18, 2023, 1:15 p.m.
    ...affect factors that could skew research results (gene expression, growth rates, etc.). Thus, it’s important...present in this line, making it genetically female. HEKs are generally cultured as an adherent monolayer...plasmids with the SV40 origin of replication. To generate a virus, you need only transfect the ‘helper’ .../fbioe.2021.796991 Additional resources on the Addgene blog Five Popular Model Organisms 10 Tips for...and Troubleshooting Additional resources on Addgene.org AAV Production in HEK-293T Cells  ...

  8. Behind-the-scenes of the Isolation of the Thermostable IgnaviCas9 From a Yellowstone Hot Spring

    Type
    Blog Post
    Published
    Nov. 12, 2019, 2 p.m.
    ...Neddersen characterized a few samples by 16S rRNA gene sequencing which showed the presence of many “dark...Yellowstone National Park (YNP). Extremophiles - phylogenetically diverse microbes living in extreme physical...lab and ThermoCas9 from the van der Oost lab. Phylogenetic comparison indicates that like GeoCas9 and ThermoCas9...particularly useful. Additional resources on the Addgene blog Learn about other Cas proteins Read all of...GeoCas9, another thermostable Cas9 Resources on Addgene.org Read our CRISPR guide Find CRISPR plasmids for...

  9. Bright Monomeric Fluorescent Proteins: mNeonGreen, mTFP1, and mWasabi

    Type
    Blog Post
    Published
    April 25, 2019, 3:01 p.m.
    ...Oregon created mTFP1 by transforming E. coli with a gene library of approximately 484 variants, including... your research Subscribe to the Addgene blog Resources on Addgene.org Learn about the Michael Davidson...Allele Biotechnology that now can be deposited at Addgene. What makes these fluorescent proteins unique and...was then screened through successive rounds of mutagenesis for the brightest teal fluorescent proteins that...scientists from Florida State University, they used a mutagenesis approach and found that specific amino acid changes...scientific community, please deposit the plasmids at Addgene! References Ai, Hui-wang, et al. "Directed evolution...PMCID: PMC3811051. Additional resources on the Addgene blog Read our fluorescent protein blog posts Find...

  10. Why Add Sucrose? Improved Yields for Adeno-associated Virus Preparation

    Type
    Blog Post
    Published
    Dec. 13, 2018, 1:14 p.m.
    ...during adeno-associated virus manufacturing." Human gene therapy21.10 (2010): 1251-1257. PMID: 20649475. ...have probably heard about Addgene’s viral service but did you know that Addgene also performs independent...101 eBook Additional resources on Addgene.org Learn about Addgene's viral service Learn about viral production...research? Since launching the viral service in 2016 Addgene’s research team has been actively investigating ...PMCID: PMC2957237. Additional resources of the Addgene blog Read our beginner's guide to viral vectors...
Showing: 501 - 510 of 928 results