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We narrowed to 837 results for: Eras

Showing: 511 - 520 of 837 results

  1. Rosella: A Fluorescent pH-Biosensor for Studying Autophagy

    Type
    Blog Post
    Published
    April 13, 2017, 2:30 p.m.
    ...cultured with isotope-labeled amino acids for several hours to several days, followed by a short period of growth...via the secretory pathway, and then cleaved to generate an active form of the protein. To monitor autophagy...Pho8Δ60 in the vacuole, where it is cleaved to generate an active enzyme. Pho8 phosphatase activity or...

  2. Stabilized Bacterial Promoters: Constant Gene Expression at any Copy Number

    Type
    Blog Post
    Published
    Sept. 4, 2018, 12:53 p.m.
    ...phenotypes. Factors such as the growth medium, growth temperature, and growth rate can all impact plasmid copy ... as the incoherent feedforward loop (iFFL). In general, iFFLs use an input signal that both positively...repression in E. coli. In addition, TALEs bind non-cooperatively to the promoter region, which is predicted to... pathway will ideally be optimized to promote generation of the desired product while avoiding the accumulation...

  3. Antibodies 101: Affinity Reagents

    Type
    Blog Post
    Published
    April 14, 2022, 1:15 p.m.
    ...alternative reagents are created in the lab, instead of generated in vivo, they have higher reproducibility and ... Fig. 1: A comparison of an antibody (left) to several alternative affinity reagents.    Types of ...small enough to be produced in bacteria and are generally cheaper to make than antibodies. Minibodies are...instead of the antibody constant region. They are generated using combinatorial libraries, and high specificity...

  4. Antibodies 101: Secondary Antibodies

    Type
    Blog Post
    Published
    Dec. 21, 2021, 2:15 p.m.
    ...antibodies work?  Secondary antibodies are antibodies generated against antibody isotypes from a specific species...classes, it is possible to get secondary antibodies generated against isotype subclasses, such as mouse IgG2...interest - the secondary antibody needs careful consideration before selection. If all the primary antibodies...my goat anti-rabbit?!”*  *a secondary antibody generated in goat against rabbit immunoglobulins. References...

  6. The Breast Cancer Microenvironment: A Tumor’s Backstage Team

    Type
    Blog Post
    Published
    Jan. 31, 2019, 1:32 p.m.
    ...there’s less malignancy because there’s less proliferation. However, the lack of caveolin 1 in breast cancer...tumor, proving once again that a tumor is always interacting with its surroundings and being shaped by them...growth mediators and improving cancer cells proliferation.  How do microenvironments affect tumor spread...regulates transcription. Without TKA1, cells proliferate and cannot undergo apoptosis, thus multiplying...

  7. Oh, The Places You Can Go: Careers in Science Communication - Writing for a Research Institute

    Type
    Blog Post
    Published
    Feb. 2, 2018, 3:20 p.m.
    ... would do if she wasn’t at the bench.  “I was literally sitting in my office one day, which I shared with...NEI), where she wrote about NEI research for a general audience. She then got a full time position with..., however, recommend using Twitter as a way to leverage unpaid work for paid work: “If you use it right...helped because there was so much work through the federal government. All the Health and Human Services (...

  8. The Future of Research Symposium Boston 2015

    Type
    Blog Post
    Published
    Oct. 20, 2015, 2:30 p.m.
    .../feudal generating too many trainees per PI” “Lack of awareness about how the system operates and functions...issues independently; for example, Harvard’s Therapeutics Graduate Program has a compulsory internship...better data is needed to help shape and inform the overall structure of the biomedical research sector, and...

  9. New Tool for Lineage Tracing: The ClonTracer Library

    Type
    Blog Post
    Published
    Sept. 22, 2015, 2:30 p.m.
    ...abundance and clonal dynamics over time using next generation sequencing (NGS). The library was developed when...such that initial resistant populations can be eradicated up-front quickly. This approach is in contrast... discovery and the development of combination therapies.         References 1. Hyo-eun, C. Bhang, et al...Studying clonal dynamics in response to cancer therapy using high-complexity barcoding." Nature medicine...

  10. 6 Tips for Grant Writing

    Type
    Blog Post
    Published
    Oct. 18, 2016, 2:30 p.m.
    ...back to you, and for you to make the relevant alterations before submitting - handing them your application...during your career; you learn new techniques and interact with new people. If you are looking to move to...non-scientists. Most fellowships are funded by the general public (either through taxes or charitable donations...an IDP Get Tips on Keeping Up With Scientific Literature  ...
Showing: 511 - 520 of 837 results