.../feudal generating too many trainees per PI” “Lack of awareness about how the system operates and functions...issues independently; for example, Harvard’s Therapeutics Graduate Program has a compulsory internship...better data is needed to help shape and inform the overall structure of the biomedical research sector, and...

...abundance and clonal dynamics over time using next generation sequencing (NGS). The library was developed when...such that initial resistant populations can be eradicated up-front quickly. This approach is in contrast... discovery and the development of combination therapies.         References 1. Hyo-eun, C. Bhang, et al...Studying clonal dynamics in response to cancer therapy using high-complexity barcoding." Nature medicine...

... permanently disable, protein-coding genes by generating insertions or deletions in the DNA sequence. ...Knock-in: Introducing tags or stop signals Once Cas9 generates double-strand breaks, a new DNA sequence can be...sequences remain intact (Engreitz et al. 2019). The interactions between ncRNAs and adjacent genes are complex...lncRNAs because they regulate nearby genes and interact with specific chromatin structures or other local...

...are planning to detect multiple proteins through several rounds of stripping and reprobing, maximize your...chemiluminescent or fluorescent detection, like horseradish peroxidase (HRP) or Alexa Fluor dyes, respectively...solution and agitate for 5–30 minutes at room temperature. The incubation time will depend on your blot...be able to strip and reprobe your western blot several times — some researchers report up to ten cycles...

...didn’t need to be strictly followed in order to generate high-fidelity reactions. (Need a refresher? The...cycling conditions (see Table 1).   Enzyme Temperature Incubation time/cycling Overhang length ...some help! References Potapov, V., Ong, J. L., Kucera, R. B., Langhorst, B. W., Bilotti, K., Pryor, J...10.1021/acssynbio.1c00525 Pryor, J. M., Potapov, V., Kucera, R. B., Bilotti, K., Cantor, E. J., & Lohman, G...

...collaborates with these companies to create and provide general availability of their recombinant antibodies. Since...that the primary antibody is very weak or with general non-specific binding. If the sample differs from...align with what the atlases (mentioned above) and literature suggest, providing an expectation and comparison...imaged pattern does NOT align with the atlases/literature and is unrelated to the target protein, we categorize...

...more than once. Fibronectin is another membrane-interacting protein that can be used to enhance transduction...aren’t working, you can check if virus is being generated by your transfected packaging cells. This check...and transduction. Once you’ve harvested, it’s generally a good idea to titer your virus. Getting an accurate...of primary human T lymphocytes for adoptive immunotherapy." Acute Leukemias VIII. Springer Berlin Heidelberg...

...involve any changes to DNA sequence (Epigenetics literally means ‘on top of the genome’). Epigenetic modifications...prevents the cellular machinery from physically interacting with the genes in the compacted region and results...can influence the transcription of genes by RNA polymerase II, or by the addition of methyl- groups to the...restriction enzyme digestion, DNA adenine methyltransferase identification, and bisulfite sequencing. ...

...expression, lentiviral, retroviral, AAV, RNAi, luciferase, cre/lox, yeast expression, worm expression, ...Growth Temp Choose from 30 °C, 37 °C, or room temperature. Growth Strain Please indicate which growth...GenBank files. We encourage submission of QUEEN-generated GenBank files (Mori and Yachie, 2021) but can ...we’ll use the information in the spreadsheet to generate plasmid pages. We’ll send you a deposit confirmation...

... its analysis can be found here: A Novel Next-Generation Sequencing and Analysis Platform to Assess the...the pool - ex. the gRNA in a CRISPR library. In general, our QC scientists work with the depositing lab...Mueller I, Mitsopoulos A, Fan M (2020) A Novel Next-Generation Sequencing and Analysis Platform to Assess the...Preparations from Viral DNA Extracts. Human Gene Therapy 31:664–678 . https://doi.org/10.1089/hum.2019.277...