We narrowed to 740 results for: NSI

...biologist--as an example of “big science,” a massive, expensive project that required collaboration from scientists...five Addgene colleagues who read it with me, the consistent response from scientist and non-scientist alike...

...For these reasons, we are in the process of transitioning our titering method from qPCR to ddPCR. Droplet...titer your AAV by ddPCR. Droplet digital PCR is a sensitive assay, so cross contamination into a no template...

...students. But he also found that his teaching responsibilities left him with limited time for one-on-one ...notes the importance of acknowledging student apprehensions about “failure” (protocols not working, hypotheses...

... Tris-buffered solution tends to produce more consistent readings — just be sure it is compatible with...these long enough?  There are, of course, more considerations and other approaches for measuring DNA quantity...

...category, will leave us with a single plasmid to consider. If you get this far and realize you need to apply...and let us help.  Check back on Thursday for an inside look at our mascot and homepage redesign. Additional...

...connections between biology and ancestral groups or, by extension, race. Many researchers do large-scale CRISPR ...potential for bias but to actively look for and consider bias as a confounding factor when interpreting...

...that is wide enough to dunk your plant’s flowers inside. 50 mL falcon tubes or plastic weigh boats are ...from a colony on a plate will reach an optimal density in 18-24 hours. I then add 0.5 mL of this starter...

...non-dividing cells. The first lentiviral vector system consisted of three plasmids: the packaging, envelope, and...capacity of a lentiviral vector but for which transient transgene expression is sufficient. Lentiviral...

...Saccharomyces cerevisiae in 1989 and are still used extensively to screen for molecular interactions in the cell...post-translationally modified, co-expression of the enzyme responsible for the modification in the assay host strain...

... Although we are busy with many other work responsibilities, we make team building activities a priority...at Addgene. In addition to her operational responsibilities, she enjoys organizing team building events...