We narrowed to 729 results for: NSI
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TypeBlog PostPublishedMay 19, 2016, 2:30 p.m....non-dividing cells. The first lentiviral vector system consisted of three plasmids: the packaging, envelope, and...capacity of a lentiviral vector but for which transient transgene expression is sufficient. Lentiviral...
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Tips for Screening with Yeast Two Hybrid Systems
TypeBlog PostPublishedOct. 22, 2015, 2:30 p.m....Saccharomyces cerevisiae in 1989 and are still used extensively to screen for molecular interactions in the cell...post-translationally modified, co-expression of the enzyme responsible for the modification in the assay host strain... -
The Importance of a Fun Workplace: Company Culture at Addgene
TypeBlog PostPublishedSept. 3, 2015, 2:30 p.m.... Although we are busy with many other work responsibilities, we make team building activities a priority...at Addgene. In addition to her operational responsibilities, she enjoys organizing team building events... -
Sharing Your Lab Protocols: Using Apps to Save Time & Track Your Experiments
TypeBlog PostPublishedSept. 23, 2014, 3:05 p.m....deposited by David Root of the Broad Institute) has consistently been one of the repository’s most frequently...Rory Macneil on Bitesize Bio Data Freedom: The Expansion of Data Sharing in Research Publications by Jim... -
Analyzing CRISPR Editing Results with ICE from Synthego
TypeBlog PostPublishedMay 8, 2018, 1 p.m....editing field by making experimental workflows considerably easier, faster, and more efficient than previous...novice to expert-level researchers in achieving consistently successful CRISPR experiments. Jessica is a ... -
Hot Plasmids - August 2020
TypeBlog PostPublishedAug. 18, 2020, 1:15 p.m....CRISPR/Cas Toolkit for plants includes 95 plasmids consisting of CRISPR/Cas nucleases, base editors, gRNA backbones...activation-based norepinephrine (GRABNE) sensors allow for sensitive and high spatiotemporal measurements of norepinephrine... -
Plasmids 101: Plasmid Incompatibility
TypeBlog PostPublishedMarch 31, 2020, 1:15 p.m....partitioning system in the bacteria, but generally it consists of a centromere-like region on the plasmid, a ...2015) Imaging centromere-based incompatibilities: Insights into the mechanism of incompatibility mediated... -
Mesothelioma - Causes, Symptoms, and Treatment
TypeBlog PostPublishedDec. 13, 2016, 3:30 p.m....from many other types of cancer. Mesothelioma is considered an orphan cancer; it is rare disease with only...non-resectable mesothelioma by the National Comprehensive Cancer Network, a coalition of the top 27 cancer... -
Transferable Skills Guide: Creativity
TypeBlog PostPublishedJan. 18, 2022, 2:15 p.m....with idea generation, try this thought exercise: Consider the issue as if there were no restraints on your...something completely unrelated to the issue and consider how it could be shaped to solve your problem. ... -
How to Be an Excellent Trainee
TypeBlog PostPublishedSept. 26, 2023, 1:15 p.m....have, then bring them to your mentor. Pro tip! Consider physically walking through the protocol, by going...receive help until you’ve already started a time sensitive experiment. Ready to fly solo? Let your bench...