Skip to main content
Addgene
Showing: 521 - 530 of 823 results
  1. 6 Tips for Grant Writing

    Type
    Blog Post
    Published
    Oct. 18, 2016, 2:30 p.m.
    ...back to you, and for you to make the relevant alterations before submitting - handing them your application...during your career; you learn new techniques and interact with new people. If you are looking to move to...non-scientists. Most fellowships are funded by the general public (either through taxes or charitable donations...an IDP Get Tips on Keeping Up With Scientific Literature  ...
  2. Illuminating Epigenetics with A FRET Based Biosensor

    Type
    Blog Post
    Published
    Nov. 19, 2015, 3:30 p.m.
    ...involve any changes to DNA sequence (Epigenetics literally means ‘on top of the genome’). Epigenetic modifications...prevents the cellular machinery from physically interacting with the genes in the compacted region and results...can influence the transcription of genes by RNA polymerase II, or by the addition of methyl- groups to the...restriction enzyme digestion, DNA adenine methyltransferase identification, and bisulfite sequencing. ...
  3. PRIDICT: Predicting Efficiencies of Prime Editing Guide RNAs

    Type
    Blog Post
    Published
    June 25, 2024, 1:15 p.m.
    ...efficiencies with pridict.it. This approach leverages the strengths of PRIDICT/PRIDICT2.0 while accounting...into play. Nicolas Mathis received his PhD from Gerald Schwank's lab at the University of Zurich (Switzerland...DeepSpCas9, a deep learning–based model with high generalization performance. Science Advances, 5(11), eaax9249..., A., Kuzmin, I., Raudvere, U., Peterson, H., Liberante, F. G., & Parts, L. (2023). Prediction of prime...
  4. Antibodies 101: Conventional vs Spectral Flow Cytometry

    Type
    Blog Post
    Published
    Aug. 3, 2023, 1:15 p.m.
    ...the beads or cells. Overall, spectral flow cytometry has the ability to generate incredible amounts of...which spectral flow cytometry requires special considerations. Conventional flow cytometry During conventional...student at Mayo Clinic studying host-microbe interactions in early life.    Resources More resources ...
  5. Quick Guide to Working with Drosophila Part 1: Getting Started with Flies

    Type
    Blog Post
    Published
    July 13, 2017, 2:30 p.m.
    ... 25°C. At low temperatures like 18°C, flies take longer to develop. At high temperatures like 30°C, flies...and Y chromosomes are miniscule, so researchers generally do not manipulate them. Essentially, the X, second...the mutation won’t drift away with each passing generation.  Another feature of balancer chromosomes is ...
  6. Anatomy of a Plasmid Page at Addgene

    Type
    Blog Post
    Published
    Feb. 4, 2016, 3:30 p.m.
    ...trail at the top of any plasmid page gives the hierarchy of web pages the plasmid page is nested in. You...it? Your best bet is the Plasmid Map: We auto-generate a plasmid map based off of the plasmid sequence...plasmids are sent as bacterial stabs at room temperature. Core of the plasmid page - Core of the plasmid...), antibiotic resistance, and optimal growth temperature are listed, as well as the name of the strain...
  7. Golden Gate Assembly Upgrades: More Fragments, Faster Assembly, and Higher Fidelity

    Type
    Blog Post
    Published
    Oct. 11, 2018, 12:30 p.m.
    ...bloggers Becky Kucera, M.Sc. and Eric Cantor, Ph.D. from New England Biolabs. Becky Kucera is a Principal...This post was contributed by guest bloggers Becky Kucera, M.Sc. and Eric Cantor, Ph.D. from New England ... more than 10 modules often rely on two-step hierarchical approaches using different Type IIS restriction...
  8. 3D Printing Meets CRISPR Cas9

    Type
    Blog Post
    Published
    Oct. 17, 2017, 12:55 p.m.
    ...that allowed researchers to create stunning, interactive images of their proteins. Since the 1980’s, journal...obscures the flexible nature of all proteins in general, and the amazing conformational flexibility that...strand. In addition, this model also displays the several Lys/Ala mutations reported by Ian Slaymaker and...classroom or trying to explain your science to the general public, visit the Center for BioMolecular Modeling...
  9. Antibodies 101: Producing Recombinant Antibodies

    Type
    Blog Post
    Published
    Aug. 13, 2024, 1:15 p.m.
    ...polyclonal antibodies are readily available from several sources, fewer sources of recombinant antibodies...appropriate buffer, it’s time to quantify it. There are several options to choose from depending on the time, reagents...2018). Aggregation can arise from a shift in temperature, pH, or salt concentration (Amin et. al., 2014... shipped to customers on ice, preventing any temperature shifts. Of course, we are also cautious during...
  10. Plasmids 101: Colony PCR

    Type
    Blog Post
    Published
    May 12, 2016, 2:30 p.m.
    ...set up a standard PCR reaction (primers, dNTPs, polymerase) using the supernatant of lysed bacteria as template...standard PCR reaction: combine template, primers, polymerase, and dNTPs and then incubate with a standard ...heating step of the PCR reaction. A standard Taq polymerase is sufficient. Controls: Controls can make ...them onto the gel. The figure above summarizes generalized expected results for the three primers previously...
Showing: 521 - 530 of 823 results