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We narrowed to 842 results for: Eras

Showing: 531 - 540 of 842 results
  1. Quickest Way to Deposit Plasmids: The Deposit Spreadsheet

    Type
    Blog Post
    Published
    March 8, 2017, 3:15 p.m.
    ...expression, lentiviral, retroviral, AAV, RNAi, luciferase, cre/lox, yeast expression, worm expression, ...Growth Temp Choose from 30 °C, 37 °C, or room temperature. Growth Strain Please indicate which growth...GenBank files. We encourage submission of QUEEN-generated GenBank files (Mori and Yachie, 2021) but can ...we’ll use the information in the spreadsheet to generate plasmid pages. We’ll send you a deposit confirmation...
  2. 6 Tips for Grant Writing

    Type
    Blog Post
    Published
    Oct. 18, 2016, 2:30 p.m.
    ...back to you, and for you to make the relevant alterations before submitting - handing them your application...during your career; you learn new techniques and interact with new people. If you are looking to move to...non-scientists. Most fellowships are funded by the general public (either through taxes or charitable donations...an IDP Get Tips on Keeping Up With Scientific Literature  ...
  3. 5 Tips for Troubleshooting Viral Transductions

    Type
    Blog Post
    Published
    Aug. 11, 2016, 2:23 p.m.
    ...more than once. Fibronectin is another membrane-interacting protein that can be used to enhance transduction...aren’t working, you can check if virus is being generated by your transfected packaging cells. This check...and transduction. Once you’ve harvested, it’s generally a good idea to titer your virus. Getting an accurate...of primary human T lymphocytes for adoptive immunotherapy." Acute Leukemias VIII. Springer Berlin Heidelberg...
  4. Illuminating Epigenetics with A FRET Based Biosensor

    Type
    Blog Post
    Published
    Nov. 19, 2015, 3:30 p.m.
    ...involve any changes to DNA sequence (Epigenetics literally means ‘on top of the genome’). Epigenetic modifications...prevents the cellular machinery from physically interacting with the genes in the compacted region and results...can influence the transcription of genes by RNA polymerase II, or by the addition of methyl- groups to the...restriction enzyme digestion, DNA adenine methyltransferase identification, and bisulfite sequencing. ...
  5. Golden Gate Assembly Upgrades: More Fragments, Faster Assembly, and Higher Fidelity

    Type
    Blog Post
    Published
    Oct. 11, 2018, 12:30 p.m.
    ...bloggers Becky Kucera, M.Sc. and Eric Cantor, Ph.D. from New England Biolabs. Becky Kucera is a Principal...This post was contributed by guest bloggers Becky Kucera, M.Sc. and Eric Cantor, Ph.D. from New England ... more than 10 modules often rely on two-step hierarchical approaches using different Type IIS restriction...
  6. 3D Printing Meets CRISPR Cas9

    Type
    Blog Post
    Published
    Oct. 17, 2017, 12:55 p.m.
    ...that allowed researchers to create stunning, interactive images of their proteins. Since the 1980’s, journal...obscures the flexible nature of all proteins in general, and the amazing conformational flexibility that...strand. In addition, this model also displays the several Lys/Ala mutations reported by Ian Slaymaker and...classroom or trying to explain your science to the general public, visit the Center for BioMolecular Modeling...
  7. Quick Guide to Working with Drosophila Part 1: Getting Started with Flies

    Type
    Blog Post
    Published
    July 13, 2017, 2:30 p.m.
    ... 25°C. At low temperatures like 18°C, flies take longer to develop. At high temperatures like 30°C, flies...and Y chromosomes are miniscule, so researchers generally do not manipulate them. Essentially, the X, second...the mutation won’t drift away with each passing generation.  Another feature of balancer chromosomes is ...
  8. PRIDICT: Predicting Efficiencies of Prime Editing Guide RNAs

    Type
    Blog Post
    Published
    June 25, 2024, 1:15 p.m.
    ...efficiencies with pridict.it. This approach leverages the strengths of PRIDICT/PRIDICT2.0 while accounting...into play. Nicolas Mathis received his PhD from Gerald Schwank's lab at the University of Zurich (Switzerland...DeepSpCas9, a deep learning–based model with high generalization performance. Science Advances, 5(11), eaax9249..., A., Kuzmin, I., Raudvere, U., Peterson, H., Liberante, F. G., & Parts, L. (2023). Prediction of prime...
  9. Antibodies 101: Conventional vs Spectral Flow Cytometry

    Type
    Blog Post
    Published
    Aug. 3, 2023, 1:15 p.m.
    ...the beads or cells. Overall, spectral flow cytometry has the ability to generate incredible amounts of...which spectral flow cytometry requires special considerations. Conventional flow cytometry During conventional...student at Mayo Clinic studying host-microbe interactions in early life.    Resources More resources ...
  10. Anatomy of a Plasmid Page at Addgene

    Type
    Blog Post
    Published
    Feb. 4, 2016, 3:30 p.m.
    ...trail at the top of any plasmid page gives the hierarchy of web pages the plasmid page is nested in. You...it? Your best bet is the Plasmid Map: We auto-generate a plasmid map based off of the plasmid sequence...plasmids are sent as bacterial stabs at room temperature. Core of the plasmid page - Core of the plasmid...), antibiotic resistance, and optimal growth temperature are listed, as well as the name of the strain...
Showing: 531 - 540 of 842 results