...Kit from John Chodera's Lab - Get high expression of His-tagged human kinases in bactera. Listen to Our...Listen to the entire Hot Plasmids episode! We generally publish 5-10 Hot Plasmid articles with each of...

...a Clinical Fellow in Pathology at Masachusetts General Hospital and Postdoctoral Fellow in the Church ...gene drives focusing on safety and the ability to generate mutant microbial strains faster than ever before... Advisory Board with a particular interest in generating Cas9 based tools to enable facile control of ...

...service to further contribute to our mission to “accelerate research and discovery.” Here are the details...in the research on the SARS-CoV-2. Nine of the overall plasmid selection will be open to industry scientists...preparation is quality controlled through next generation sequencing and spectrophotometry to test purity...

...researchers facilitate and accelerate scientific discovery. How can Addgene help accelerate your research? Let...Addgene headquarters every day. That would be an average of 673 plasmids each day. This requires tremendous...

...dual-nuclease Cas9-Cas9 chimeras Scot Wolfe’s lab has developed dual-nuclease Cas9-Cas9 chimeras with higher target... in Nature Communications Find the Cas9-Cas9 chimera plasmids A modular toolkit for genome editing...

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...Twenty years of accelerating scientific discovery. Over 150,000 plasmids empowering researchers worldwide...sequencing capabilities to complement our next-generation sequencing, setting new standards for plasmid... not just sharing research materials — we're accelerating the future of science. Want to learn more about...

...and in my personal experience, is a quick way to generate many mutated plasmid constructs. The second post...and discusses a newer, CRISPR-based technique to generate C->T or G->A transitions in a target sequence ...potential specificity issues, and other means of generating mutations in live cells are all discussed further...

... and PTPN2, which are of interest as cancer immunotherapy targets but are embryonic lethal, hindering ... titer lentivirus preparations than the first generation CHIME (pXPR_053). Find X-CHIME plasmids here!...., & Sharpe, A.H. (2019). PTPN2 regulates the generation of exhausted CD8+ T cell subpopulations and restrains...

...communicating science to the general public and training the next generation of science communicators. In... is a source of scientific information for the general public as well as a platform for scientists to ...