An “elegans” Approach to Better CRISPR/Cas9 Editing Efficiency
Type
Blog Post
Published
Dec. 17, 2020, 1:30 p.m.
...demonstrated that selection for repair of a temperature-sensitive pha-1 mutation significantly enriches for knock-in...from a single injected animal.
PAM sequence considerations
For changes 5’ to the PAM, sequences corresponding...