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We narrowed to 842 results for: Eras

Showing: 551 - 560 of 842 results
  1. Scientific Reproducibility - Focusing on Solutions at the Minisymposium on Reproducibility

    Type
    Blog Post
    Published
    May 18, 2018, 7:32 p.m.
    ...of understanding how the body works, generating new therapeutics, and sometimes formulating new biological...Bio-protocol all work to provide more descriptive and interactive protocols that make it much easier to learn new...Initial positive results and the excitement they generate can, however, bias us. We’ve all heard stories...
  2. Plasmids 101: Restriction Cloning

    Type
    Blog Post
    Published
    Feb. 18, 2016, 3:42 p.m.
    ...binds the plasmid to the new fragment thereby generating a complete, circular plasmid that can be easily...green, insert) are cut with restriction enzymes to generate compatible overhangs that allow them to bind. ...single tube allowing the compatible overhangs generated by restriction digestion to anneal to one another...midiprep or a maxiprep. In these purifications, you generally lyse the bacteria; add chemicals to precipitate...
  3. The Twelve Days of CRISPR

    Type
    Blog Post
    Published
    Dec. 22, 2020, 2:15 p.m.
    ...base editing from the first generation of base editors to the eighth generation (and dual base editors, too...been updating many of these posts over the last several months. Now the #12DaysOfCRISPR is back on Twitter...
  4. How to Deposit Your Plasmids with Addgene

    Type
    Blog Post
    Published
    Feb. 27, 2024, 2:15 p.m.
    ...process, simply enter your preprint title or a general description of the deposit. Select your desired...millions. Pooled libraries must be verified by next-generation sequencing. Please see the help center article...that can be used under BSL1 conditions were generated in your lab are necessary for use of a plasmid...need information on how to verify any genomic alterations unique to your strain. Please contact us at deposit...
  5. New Podcast Segment: Hot Plasmids

    Type
    Blog Post
    Published
    May 17, 2017, 3:37 p.m.
    ...Kit from John Chodera's Lab - Get high expression of His-tagged human kinases in bactera. Listen to Our...Listen to the entire Hot Plasmids episode! We generally publish 5-10 Hot Plasmid articles with each of...
  6. Alex Chavez on MD PhDs, CRISPR, Gene Drives, & More

    Type
    Blog Post
    Published
    Nov. 15, 2016, 3:30 p.m.
    ...a Clinical Fellow in Pathology at Masachusetts General Hospital and Postdoctoral Fellow in the Church ...gene drives focusing on safety and the ability to generate mutant microbial strains faster than ever before... Advisory Board with a particular interest in generating Cas9 based tools to enable facile control of ...
  7. Celebrating One Million Plasmids Shared!

    Type
    Blog Post
    Published
    Sept. 25, 2018, 2:39 p.m.
    ...researchers facilitate and accelerate scientific discovery. How can Addgene help accelerate your research? Let...Addgene headquarters every day. That would be an average of 673 plasmids each day. This requires tremendous...
  8. Addgene’s Newest Way to Speed Your Research: The Cloning Grade DNA Service

    Type
    Blog Post
    Published
    July 28, 2020, 1:15 p.m.
    ...service to further contribute to our mission to “accelerate research and discovery.” Here are the details...in the research on the SARS-CoV-2. Nine of the overall plasmid selection will be open to industry scientists...preparation is quality controlled through next generation sequencing and spectrophotometry to test purity...
  9. What's New in CRISPR - Winter 2018

    Type
    Blog Post
    Published
    Dec. 18, 2018, 1:17 p.m.
    ...dual-nuclease Cas9-Cas9 chimeras Scot Wolfe’s lab has developed dual-nuclease Cas9-Cas9 chimeras with higher target... in Nature Communications Find the Cas9-Cas9 chimera plasmids A modular toolkit for genome editing...
  10. How Do I Place an Addgene Order Using a PO Number?

    Type
    Blog Post
    Published
    Feb. 27, 2020, 2:15 p.m.
    ...information that is entered at checkout is used to generate the legal agreement (MTA), which streamlines the...Pay by Purchase Order.” Enter the PO number you generated through your organization in this field. Please...purchasing department requires a quote in order to generate a PO, learn more about how to create a quote. ...
Showing: 551 - 560 of 842 results