...resource developed by the Biological General Repository for Interaction Datasets (BioGRID) in order to increase... Users can cross-interrogate screens from the literature for a drug, disease, or condition of interest...

...Addgene is a non-profit, we can focus more on generating blog content that scientists will find useful...giving it a high five. While screaming can be therapeutic and I’ve certainly done my fair share of venting...management, information distillation, etc) are transferable to many different fields. If you start thinking...

...inefficient process– just 1 out of 10,000 cells on average! Without some means of quickly determining which...resistance!  What are antibiotics? Antibiotics are generally defined as agents that kill bacteria, or inhibit...lab, their mechanism for killing bacteria, and general working concentrations. For instructions on how...

...biological tools, and with the right safety considerations, they aren't dangerous for controlled lab usage...and episomal expression will last ~2-7 days at moderate levels. With regard to target cells or organism...Transduce on! Resources Resources on Addgene.org Generating Stable Cell Lines with Lentivirus Virus Handling...

...highlights from the past couple months including several pooled libraries: Cas13X and Cas13Y, two compact...It was designed based on MYC-ChIP-seq data from several MYC-dependent cancer cell lines. It can be applied...the new viral preps from recent months: Second generation GRAB dopamine sensors from Yulong Li’s lab are...

...screening, and next-generation sequencing of hybridomas have opened the door for a new generation of recombinant...Animal-based methods for antibody production have several drawbacks. Polyclonal antibodies produced in animals...

...fluorescent dyes for DNA-protein interaction studies? Kyubong Jo's lab generated fluorescent protein fusions...lentiviral vectors the iBARs can be detected by next generation sequencing.  In order to avoid a high false-positive...

...translate to a desire to do the research itself. Several years of exclusively writing lab reports and empirical...busy point of the first semester in which I had several projects nearing deadline (an animated video depicting...chance to carry out a comprehensive review of the literature informing the notion of “heading” in soccer as...

...find some proteins of interest for which you have several antibodies in stock. Why are there so many options...let’s think about induced conformation changes. Several types of assays, such as ELISAs and Westerns, frequently...complex if you’re looking for proteins that are interacting with one another, or if you’re looking for a ...

...unfold, absorbing the stochasticity of molecular interactions and embedding individual diversity to get from...than what fits for applied research. I focus in general terms on a couple of very broad topic domains, ...networks, as much as with technical knowledge. I leverage a breadth of knowledge to put diverse aspects ...