We narrowed to 837 results for: Eras

1. Walking beside Academia and giving it a high five: My transition from graduate work to a position at Addgene

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   Blog Post

   Published

   July 2, 2015, 8:37 p.m.

   ...Addgene is a non-profit, we can focus more on generating blog content that scientists will find useful...giving it a high five. While screaming can be therapeutic and I’ve certainly done my fair share of venting...management, information distillation, etc) are transferable to many different fields. If you start thinking...

2. Plasmids 101: Antibiotic Resistance Genes

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   Blog Post

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   Jan. 30, 2014, 3:29 p.m.

   ...inefficient process– just 1 out of 10,000 cells on average! Without some means of quickly determining which...resistance!  What are antibiotics? Antibiotics are generally defined as agents that kill bacteria, or inhibit...lab, their mechanism for killing bacteria, and general working concentrations. For instructions on how...

3. Hot Plasmids - June 2020 - Barcoded CRISPR Library, Sparse Cell Labeling, Calcineurin Reporter, and DNA Staining Dye Alternative

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   Blog Post

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   June 2, 2020, 1:15 p.m.

   ...fluorescent dyes for DNA-protein interaction studies? Kyubong Jo's lab generated fluorescent protein fusions...lentiviral vectors the iBARs can be detected by next generation sequencing.  In order to avoid a high false-positive...

4. Summer SciComm Series: Master of SciComm

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   Blog Post

   Published

   July 21, 2022, 1:15 p.m.

   ...translate to a desire to do the research itself. Several years of exclusively writing lab reports and empirical...busy point of the first semester in which I had several projects nearing deadline (an animated video depicting...chance to carry out a comprehensive review of the literature informing the notion of “heading” in soccer as...

5. Antibodies 101: Epitope Availability

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   Blog Post

   Published

   Jan. 11, 2022, 2 p.m.

   ...find some proteins of interest for which you have several antibodies in stock. Why are there so many options...let’s think about induced conformation changes. Several types of assays, such as ELISAs and Westerns, frequently...complex if you’re looking for proteins that are interacting with one another, or if you’re looking for a ...

6. Hot Plasmids and Viral Preps - September 2021

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   Blog Post

   Published

   Sept. 21, 2021, 1:15 p.m.

   ...highlights from the past couple months including several pooled libraries: Cas13X and Cas13Y, two compact...It was designed based on MYC-ChIP-seq data from several MYC-dependent cancer cell lines. It can be applied...the new viral preps from recent months: Second generation GRAB dopamine sensors from Yulong Li’s lab are...

7. Addgene Receives NIH BRAIN Initiative Grant to Create Open-Access Recombinant Antibody Resource

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   Blog Post

   Published

   May 25, 2021, 2:29 p.m.

   ...screening, and next-generation sequencing of hybridomas have opened the door for a new generation of recombinant...Animal-based methods for antibody production have several drawbacks. Polyclonal antibodies produced in animals...

8. Using Addgene's New Viral Service for Your Research

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   Blog Post

   Published

   Nov. 17, 2016, 3:30 p.m.

   ...testing at Addgene meaning they come ready made to accelerate your research.   Why distribute virus? Plasmid...This service will further enable scientists and accelerate research." What viral preps are available...expression of a gene under the control of the tet operator. AAV AAVs are the workhorses of both chemo- and...

9. Viral Vectors 101: Transductions

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   Blog Post

   Published

   April 30, 2024, 1:15 p.m.

   ...biological tools, and with the right safety considerations, they aren't dangerous for controlled lab usage...and episomal expression will last ~2-7 days at moderate levels. With regard to target cells or organism...Transduce on! Resources Resources on Addgene.org Generating Stable Cell Lines with Lentivirus Virus Handling...

10. Science Careers: Unruly Interests Feed Many Paths

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    Blog Post

    Published

    Sept. 26, 2017, 12:12 p.m.

    ...unfold, absorbing the stochasticity of molecular interactions and embedding individual diversity to get from...than what fits for applied research. I focus in general terms on a couple of very broad topic domains, ...networks, as much as with technical knowledge. I leverage a breadth of knowledge to put diverse aspects ...