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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 837 results for: Eras

Showing: 581 - 590 of 837 results

  1. Don’t Wait To Launch Your Health Science Startup

    Type
    Blog Post
    Published
    Aug. 25, 2015, 2:30 p.m.
    ... due to a few limiting factors.  To start, the average health science startup requires a great deal more...those ideas into commercial products and next generation companies.  We launched in the early spring of...(CFO).  As CEO, I am responsible for internal operations and building external strategic partnerships ...

  2. Another Pathway into Cells: iTOP

    Type
    Blog Post
    Published
    June 23, 2015, 8:37 p.m.
    ...enter cultured cells and activate expression of a luciferase reporter. CPP was not required for protein entry...cells! After verifying the specificity of their luciferase assay, they concluded that components in the ...lethality and were sorted into single wells to generate clonal populations. After iTOP treatment, both...

  3. Extracurricular Activities for a Strong Science Career Path

    Type
    Blog Post
    Published
    Jan. 21, 2014, 3:08 p.m.
    ...However, careers outside of the lab highlight “transferable” skills such as leadership, management, and ...impact your work? What design variables need consideration? Enrich your knowledge and skills outside of...tools such as the Individual Development Plan to generate a list of potential professions Google potential...

  4. What Good Citizenship Can Do for Reproducibility in Science

    Type
    Blog Post
    Published
    Nov. 7, 2019, 2:18 p.m.
    ...minor variation in experimental conditions will derail a research program (think a subtle difference in...to help your fellow researchers advance science overall. Communication is the issue Much of the problem...President. Her current responsibilities encompass overall leadership of the Cell Press editorial department...

  5. Celebrate National Book Month with Addgene

    Type
    Blog Post
    Published
    Oct. 3, 2019, 12:47 p.m.
    ..., why it affects our emotions, and even what therapeutic properties it has. As with other works of Dr....of characters from five different families over several millennia as their paths cross with each other ...Nicholson Baker’s The Anthologist. The book ended up literally changing my life. Though I have an undergraduate...

  6. Quantifying DNA? Here are Five DNA Quantification Methods to Consider

    Type
    Blog Post
    Published
    July 21, 2020, 1:15 p.m.
    ...reading, you can calculate the DNA concentration. Generally, A260 of 1.0 is equivalent to 50 ug/ml pure dsDNA...samples and are often used to quantify DNA for next generation sequencing. Unlike absorbance-based methods, .... This method is often used preceding next-generation sequencing or microarray studies and is not used...

  8. How Dry Ice Affects Viral Vector Sample pH & How to Avoid It

    Type
    Blog Post
    Published
    June 20, 2017, 2:30 p.m.
    ...-rings are, in fact, permeable to CO2 at low temperatures (-80°C) and once in the tube, the CO2 can alter... storage, we then thawed the samples at room temperature and used the color of the liquid solution and...not all O-rings) were permeable to CO2 at low temperatures. We tested two different types of tubes: the...

  9. Viral Vectors 101: Inverted Terminal Repeats

    Type
    Blog Post
    Published
    Oct. 19, 2023, 1:15 p.m.
    ...in a cell or animal model. AAV plasmids contain several important elements, but among the most important...occurring.  How do I validate my ITR sequences?  Next-generation sequencing and Sanger sequencing might fail to...vector stability and sequencing results. Next-generation sequencing is also helpful in quantifying ITR...

  10. Hot Plasmids - January 2023

    Type
    Blog Post
    Published
    Jan. 31, 2023, 2:15 p.m.
    ... need a second GFP antibody?  Similarities:  Generated against full length GFP and converted to recombinant... also withstands denaturing conditions, high temperatures, and other chaotropic conditions better than...fluorescence in high molarity GdnHCl, at high temperatures, and in the presence of oxidizing agents. Adapted...
Showing: 581 - 590 of 837 results