...sample. The second sensor is a fusion protein consisting of sfGFP fused to a His-tagged ABC transporter...exposed to ultraviolet light. To do so, she generated fusion proteins in which Dronpa domains flanked either...either side of an enzyme’s active site. In these fusions, ultraviolet light exposure caused oligomerization...iterations of FLiPs only used N- and C-terminal dronpa fusions. With these various designs, Xin has been able ...

... own. When the two fragments come together, they fuse and fluoresce. The team engineered C. elegans cell...2020.07.02.185249 Predicting cell cycle length with a histone-fused fluorescent timer By Kate Harten DeMaio Studies ...Shangqin Guo and her colleagues at Yale University fused a color-changing fluorescent timer (FT) protein ...hours in primary mouse embryonic fibroblasts. This fusion protein can be used to determine cell cycle length...lentiviral preps at Addgene hSyn1-SIO-stGtACR1-FusionRed is a Cre-dependent, soma-targeting inhibitory ...

...Moshe Szyf at McGill University. Some enzymatic fusions are godsends. Tethering dCas9 to the histone acetyltransferase...where they were absent before. But other enzymatic fusions, such as dCas9-TET, can, like the iron ball-and-chain...notoriety – as a tool of choice in the field: dCas9 fused to the enzymatic domain of TET1 or TET2 (Morita ...by dCas9 alone, without TET (or any demethylase) fusion. This may seem counterintuitive: how can a DNA-...–peptide repeat and scFv–TET1 catalytic domain fusions. Nat Biotechnol 34, 1060–1065. https://doi.org/...

...purified by centrifugation. Gag-Cas9 fusion protein The MLV Gag protein fused to a Flag-tagged SpCas9 is incorporated...transcriptional activator dCas9-VPR, if these variants are fused to the MLV Gag in place of SpCas9. gRNA A gRNA targeting...association with the the Cas9 portion of the Gag-Cas9 fusion protein. A single Nanoblade particle can be loaded...space surrounding the zygote, where they can then fuse with the target cell membrane, and transfer their...

... that they only facilitate degradation of their fused protein under certain conditions (typically the ...novel degrader of the 12 kDa protein FKBP12F36V. By fusing FKPB12F36V to your protein of interest, it can ...molecule which induces dimerization of the FKPB12F36V fusion protein with the endogenous CRBN E3 ligase complex...complex which will polyubiquitinate the fusion protein. This will target the protein of interest to the ... molecules induce dimerization of an FKBP12F36V fused target protein-of-interest and the CRBN E3 ligase...

... perfectly capable of synthesizing fluorophore fusions on their own. Subsequent advances in fluorescent...moving from transient overexpression of protein fusions to gene-edited cells can decrease the number of... (1) a genetically encoded “tag” expressed as a fusion with your favorite protein and (2) a synthetic ...small-molecule labeling approaches can provide a fresh infusion of photons for your imaging experiment. Innovative...Resources on Addgene.org Generate Fluorescent Protein Fusions Request Plasmids from the Michael Davidson Collection...

...constantly being developed! Fusion tagging: One of the most common uses, GFP can be fused to the N- or C-terminus...of empty backbones for constructing fluorescent fusions. Transcription Reporter: Placing GFP under the ...overlapping excitation/emission spectra are used; one fused to each protein or domain being tested. Find FRET...interactions. In this case, two portions of EGFP are fused to the proteins of interest, and when they come ...

... of E. coli tRNA adenosine deaminases (ecTadA), fused it with dCas9, and transformed it into E. coli harboring...and tomato using a CRISPR-Cas9 cytidine deaminase fusion." Nature biotechnology 35.5 (2017): 441. PubMed... wheat and maize with a Cas9-cytidine deaminase fusion." Nature biotechnology 35.5 (2017): 438. PubMed...Efficient C-to-T base editing in plants using a fusion of nCas9 and human APOBEC3A." Nature biotechnology...rice and wheat using a Cas9-adenosine deaminase fusion." Genome biology 19.1 (2018): 59. PubMed PMID: ...

...catalytically dead Cas9 (dCas9) alone or a dCas9 fused to the omega subunit of RNA polymerase, the lab   ...for CRISPRi and CRISPRa using RNAP-Omega-dCas9 fusions. Here’s some others. SoxS based CRISPRa In the ...The authors were able to engineer a dCas9 variant fused to bacterial enhancer binding protein required for...editing. This process is generally accomplished by fusing an enzyme capable of converting one base to another...identified a cytidine deaminase, PmCDA1, that could be fused to dCas9 and efficiently introduce cytosine-to-thymine...glycosylase inhibitor and degradation tag on the fusion protein to limit the activity of the system as ...

...binding scFv that’s fused to GFP. When the multiple copies of the scFv-GFP fusion bind the SunTag scaffold...smaller than antibodies. scFvs are often genetically fused to other proteins, which has led to their application...detection probe. It contains an HA-binding scFv that’s fused to a fluorescent protein such as GFP. The HA frankenbody...brings T cells to a tumor site. ScFvs can also be fused to cellular toxins, radioisotopes, cytokines, and...