We narrowed to 291 results for: Pol

...Modular by design: Key elements (promoter, tag, polyA site) are flanked by unique restriction sites so...general heat-shock proteins using IP with CycT1 or PolII-CTD antibodies and unlabeled MS in DLD-1 cells (...

...Wednesday we worked with the Harvard GSAS Science Policy Group to organize a Minisymposium on Reproducibility.... Down the line we may see further adoption of policies that promote reproducible publishing practices...

...Promoters control the binding of RNA polymerase and transcription factors. Since the promoter region ...guide to working with Drosophila Terminators and PolyA Signals FLEx Vectors Resources at Addgene.org ...

...105 colonies. Pro Tip: Use a high-fidelity Taq polymerase with proof-reading ability for generating dsDNA...nucleotides 1 out of 104 to 105 Use a high fidelity Taq polymerase to generate insert ssDNA Point mutations or...

... development, business development, consulting, policy, big data and the list goes on and on. As you think...Hire American" Executive Order which sets broad policy intentions directing federal agencies to propose...

...thaliana dCas9 Activation of gene transcription Polstein et al., 2015 LACE CRY2 and CIB1 from A. thaliana...https://doi.org/10.1016/j.chembiol.2014.12.011 Polstein LR, Gersbach CA (2015) A light-inducible CRISPR-Cas9...

... or a dCas9 fused to the omega subunit of RNA polymerase, the lab   successfully repressed or activated... al., 2014). These could be genes, specific polymorphisms, including antibiotic resistance genes. This...

...of assembling these multi-protein fusions or “polyproteams”, the Howarth lab created the MBPx-SpyCatcher...sequential additions have been made, the final polyproteam can be eluted using maltose. This procedure allows...

... Gut to BrainResearchers: Atharva Sahasrabudhe; Polina Aniveeka   A big thank you to all who have contributed...

... pcDNA-SADB19L Contains rabies viral RNA polymerase; see depositing paper for more information Edward...

...ladder works well for both agarose and native polyacrylamide gels. Get Tips on Verifying Your Plasmid ...

...head over to Twitter to vote on @addgene's Twitter poll. Voting is open until Feb. 22, 2022, and we'll unveil...

...not. We will have Addgene trivia (with prizes!), polls, and shout outs, and we’ll be featuring the work...

...Jensen New serotype pAAV-CAG-2xNLS-tdTomato-WPRE-hGHpolyA AAV9, PHP.eB Controls Feng New viral service, ...

...against a target antigen. This contrasts with polyclonal antibodies, which are mixtures of monoclonal ...

...be entirely endotoxin free. Endotoxin is a lipopolysaccharide of the cell wall of Gram negative bacteria...

...R. A., Virk, H., Laflamme, C., Houston, D. W., Polinski, N. K., Meijers, R., Levey, A. I., Saper, C. B...

... February, the Office of Science and Technology Policy issued a memorandum calling on Federal agencies...

...these may require restriction fragment length polymorphism analysis, allele-specific PCR, T7EN1 cleavage...

...advance COVID-19 research to hopefully help guide policies and ultimately treatments in a timely fashion....

...expertise in law, business, journalism, entertainment, politics, K-12, etc.” Addgene's team includes many PhD ...

...has you covered! His His tags, often called polyhistidine tags, typically include six consecutive His ...

...patterns, post-translational modifications and polymorphisms Codon optimization tool: To access this tool...

... with my teammates acted as my safety net and trampoline all at once. I was able to do something that ...

...have faster off kinetics and allow neurons to repolarize faster.  Chemogenetics does not offer the same...

...molecular biology like restriction enzymes and polymerases. In the situation where you need to use plasmid...

..., as well as to optogenetically control the depolarization of R2 neurons using CsChrimson::tdTomato, an...

...BitesizeBio is the protocol for making your own Taq polymerase by Outreach Scientist, Andy Baltus. Addgene founder...

...blog Antibodies 101: Validation Antibodies 101: Polyclonal Antibodies Antibodies 101: Selecting the Right...

..., management consulting, science communication, policy and the diverse options in nonprofit science. No...