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TypeBlog PostPublishedNov. 13, 2015, 3:30 p.m....Addgenies represent. They kicked off their day with breakfast and an introduction to Addgene with our Executive...
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Download Science-Themed Zoom Backgrounds from Addgene
TypeBlog PostPublishedApril 22, 2021, 1:15 p.m....helping us make Plasmids 101 videos and needed a break so they're hanging out with Aavery in this Zoom ... -
Measuring Kinase Activity at the Single-Cell Level with Kinase Translocation Reporters (KTRs)
TypeBlog PostPublishedJuly 26, 2018, 12:46 p.m.... activity that occurs during nuclear envelope breakdown (without the nucleus, there’s no nuclear localization... -
Fluorescent Proteins 101: History of Fluorescent Proteins
TypeBlog PostPublishedAug. 7, 2017, 1:58 p.m.... in molecular detail. On the underside of the “umbrella” of A. victoria there is a ring that glows with... -
What's New in CRISPR - Spring 2019
TypeBlog PostPublishedMarch 21, 2019, 12:47 p.m....that allows it to be repurposed for double strand breaks at target sites. Read the paper in Nature Communications... -
Download Addgene’s Science Career Guide, Plus 8 Career Tips from Addgenies
TypeBlog PostPublishedFeb. 26, 2019, 1:40 p.m....Searching for a new job can be an imposing endeavor. Break the process down into steps and set aside time during... -
Quick, Versatile Plant Transgenesis with GreenGate Plasmids
TypeBlog PostPublishedJan. 23, 2014, 3:23 p.m....researchers introduced what’s been called a major breakthrough in cloning technology with the Golden Gate method... -
The 10 Most Distributed Plasmid Technologies in Addgene's First 10 Years
TypeBlog PostPublishedJan. 8, 2014, 3:10 p.m....Addgene. Thank you for inspiring us with your breakthrough research and your commitment to scientific sharing... -
Our Most Popular Blog Posts from 2019
TypeBlog PostPublishedDec. 31, 2019, 2:26 p.m.... Addgene depositor and guest blogger Erik Snapp breaks down how to prepare a chalk talk for an academic... -
Top Requested AAV of 2017: pmSyn1-EBFP-CRE
TypeBlog PostPublishedJan. 17, 2018, 2:57 p.m....controlled by a neuron specific promoter or you could breed your Cre-dependent mCherry line with a separate ... -
Editor's Choice, September 2016
TypeBlog PostPublishedOct. 7, 2016, 4 p.m....what new post contributed the most to this record breaking month and to find other posts that deserve a second... -
Management for Scientists: Seeking Feedback
TypeBlog PostPublishedJan. 8, 2015, 2:34 p.m....going on and can contribute. Members periodically break, go exploring outside the team, and bring information... -
CRISPR Protocol for Genomic Deletions in Mammalian Cell Lines [Video]
TypeBlog PostPublishedFeb. 18, 2015, 3:09 p.m....Cas9, we began to explore the paired double-strand break (DSB) approach at a variety of loci. We were pleasantly... -
Three Tips to Organize Your Lab Notebooks in the New Year
TypeBlog PostPublishedJan. 2, 2014, 3:44 p.m.... your first day back in the lab after a holiday break filled with family, food, and travel, and you can... -
Tag Your Favorite Yeast Genes with Ease
TypeBlog PostPublishedNov. 19, 2013, 2:37 p.m....repair genomic damage, specifically double strand breaks. Researchers have long taken advantage of this ... -
Supporting Rare Disease Research with BeHeard 2017
TypeBlog PostPublishedJuly 20, 2017, 2:30 p.m....We often hear about breakthroughs in diabetes and lymphoma research or the many different ways you can... -
Increasing Your Work's Visibility with Addgene: Citation, Search, and Collections
TypeBlog PostPublishedMarch 10, 2016, 3:30 p.m....on the right side of the search page. A great breakdown of our search functionality can be found in the... -
pSiM24: Simplifying Plant Genetic Engineering
TypeBlog PostPublishedSept. 29, 2015, 2:30 p.m....important foundation for life on Earth. Selective breeding methods have shaped the plants that we grow and... -
Celebrating Outstanding CRISPR/Cas9 Achievements at the Dr. Paul Janssen Award Dinner
TypeBlog PostPublishedSept. 15, 2014, 6:05 p.m....emphasized the potential of this extraordinary breakthrough. In fact, the panelists agreed that, for example... -
London Calling - Addgene’s New Europe Office in London
TypeBlog PostPublishedJune 10, 2014, 3:20 p.m....using Bioluminescence Resonance Energy Transfer (BRET) technique, and plasmids to study the expression... -
CRISPR 101: Cas9 Nickase Design and Homology Directed Repair
TypeBlog PostPublishedMarch 15, 2018, 12:59 p.m....create a single-strand rather than a double-strand break, and when used with two adjacent gRNAs, can lower... -
Oh, The Places You Can Go: Careers in Science Communication - Science Writing
TypeBlog PostPublishedOct. 3, 2017, 12:11 p.m....’s biggest piece of advice for those looking to break into the field of science communications-- Be able... -
An Addgene Summer Internship
TypeBlog PostPublishedAug. 8, 2024, 7:22 p.m....week at Addgene. This report shared my work by breaking down the process of each day, my background, observations... -
Chemogenetics vs. Optogenetics: Which Method Should I Choose?
TypeBlog PostPublishedJune 11, 2020, 1:34 p.m....Optogenetic approaches excel at discrete cell or tissue-subregion targeting down to the limits of light delivery... -
CRISPR 101: Drug discovery and CRISPR
TypeBlog PostPublishedMay 4, 2023, 1 p.m....you identified gene target X for BRCA-deficient breast cancer and identified several candidate inhibitors... -
New CRISPR Web Resources and #12DaysofCRISPR Recap
TypeBlog PostPublishedDec. 12, 2017, 3:13 p.m....>G:C edits in DNA without causing double-strand breaks. Our new page shows you base editing plasmids for... -
Tips for Improving Your Next Manuscript
TypeBlog PostPublishedNov. 22, 2016, 3:30 p.m.... The most beneficial aspect of this course was breaking up into focus groups with a facilitator. In the... -
Sleeping Beauty Awakens for Genome Engineering
TypeBlog PostPublishedJune 30, 2015, 2 p.m....cell.4.) The transposase creates a double strand break in the DNA and allows the tranposon to integrate... -
Addgene @ Keystone: Thoughts on Precision Genome Engineering and Synbio
TypeBlog PostPublishedJan. 15, 2015, 1:50 p.m....meeting, and its history of code making and code breaking is wonderfully analogous to genetic circuits discussed... -
Advice for Choosing a Research Project
TypeBlog PostPublishedApril 17, 2014, 2:45 p.m....topic that “looks at the complexity of biology and break apart a piece that can address a topic in a mechanistic...