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We narrowed to 920 results for: Gene

Showing: 201 - 240 of 920 results

  1. Plasmids 101: Antibiotic Resistance Genes

    Type
    Blog Post
    Published
    Jan. 30, 2014, 3:29 p.m.
    ...region of the gene they are trying to disrupt or delete, which both inactivates the gene and acts as a...maintain the plasmid. Adding an antibiotic resistance gene to the plasmid solves both problems at once – it...Historically, antibiotics have also been used to disrupt genes at the chromosomal level. Scientists introduce an...typically has two separate resistance cassettes and your gene of interest is cloned into/inactivates or completely...resistance!  What are antibiotics? Antibiotics are generally defined as agents that kill bacteria, or inhibit...lab, their mechanism for killing bacteria, and general working concentrations. For instructions on how...how to prepare antibiotic stocks, see Addgene's Reference Page. How else can antibiotics be used in the...

  2. What's New in CRISPR - May 2019

    Type
    Blog Post
    Published
    May 28, 2019, 12:49 p.m.
    ... gRNAs) that targets ~4,000 E. coli genes with ~15 gRNAs per gene and includes ~10,000 gRNAs targeting...CRISPR interference (CRIPSRi) platform for targeted gene repression in Candida albicans was developed by ... transcription of HSP90 an essential C. albicans gene. Read the paper in mSphere Find the dCas9 and... a few of the new CRISPR plasmids available at Addgene. We will still periodically focus on specific CRISPR...a new CRISPR tool you’ve recently deposited to Addgene and you’d like it to be included in the next What...more information. Additional resources on the Addgene blog Download the CRISPR 101 eBook Find our CRISPR...posts Read our CRISPR cheat sheet Resources on Addgene.org Read our CRISPR guide Find CRISPR plasmids by...

  3. CRISPR-Cas14: a family of small DNA-targeting enzymes enabling high-fidelity SNP genotyping

    Type
    Blog Post
    Published
    Nov. 29, 2018, 1:53 p.m.
    ...Energy’s Joint Genome Institute for uncharacterized genes that were proximal to a CRISPR array and the universal...CRISPR-Cas systems containing cas1, cas2, cas4, and a new gene cas14. cas14 encodes for a small Cas protein (40...-Cas systems. There are 24 variants of the cas14 gene that cluster into 3 subgroups (cas14a-c). All these...potential of Cas14, the Doudna lab cloned the cas14 gene together with the adjacent CRISPR array and intergenic...presence of infectious organisms (ex: SARS-CoV-2) and genetic mutations. By providing a gRNA specific of a viral...ID PMID: 30337455. Additional resources on the Addgene Blog Learn more about DETECTR Browse all of the...Download our CRISPR cheat sheet Resources on Addgene.org Find the Cas14 plasmids here Checkout our CRISPR...

  4. Negative Can Be Positive: Open AAV Data with Addgene

    Type
    Blog Post
    Published
    Sept. 6, 2022, 1:15 p.m.
    ...Adeno-Associated Viral Vector-Mediated Gene Delivery to the Hypothalamus. Human Gene Therapy, 21(6), 673–682. https...conditions commonly encountered in human gene therapy trials. Human Gene Therapy Methods, 26(2), 71–76. https...Grames, M. S., & Klein, R. L. (2018). More expansive gene transfer to the rat CNS: AAV PHP.EB vector dose–...dose–response and comparison to AAV PHP.B. Gene Therapy, 25(5), 392–400. https://doi.org/10.1038/s41434-018...Adeno-Associated Virus Serotype 9 (AAV9) Preparations. Human Gene Therapy Methods, 30(6), 214–225. https://doi.org...Resources on addgene.org  Download Addgene's Viral Vectors 101 e-book Additional Resources on the Addgene Blog... by Addgene in 2021 asking scientists what data would improve our AAV Data Hub.   The Addgene Data...

  5. Viral Vectors 101: The Retroviral Lifecycle

    Type
    Blog Post
    Published
    July 27, 2023, 1:15 p.m.
    ... are, in most cases, interested in transferring genes to mammalian cells (i.e. transferring DNA), yet ...infection; however this would not be great for a gene therapy vector! For viral vectors, only the protein... is (thankfully) gutted of many essential viral genes. How it is, exactly, that heaps of viral vector ... particles are made while missing the polymerase gene may seem like a mystery. But it’s simple. The RT...the only component that is not dependent on viral genes. It is derived from the host/producer cell membrane...full “life cycle” of expression of selfish, lazy genes of a non-living viral particle, and how we as dedicated...Kantor, B. (2021). Lentiviral Vectors for Delivery of Gene-Editing Systems Based on CRISPR/Cas: Current State...

  6. Human Germline Editing Using CRISPR

    Type
    Blog Post
    Published
    Aug. 10, 2017, 2:19 p.m.
    ...dominant disease-causing gene, or that carry only one copy of a recessive gene. Ma et al. claim that CRISPR...Variants Target Multiple Genes at Once with CRISPR Resources on Addgene.org Browse All CRISPR Plasmids...authors to title the paper “Correction of a pathogenic gene mutation..” rather than using term ‘editing.’ While...but this advantage likely won’t transfer to all genes we’d like to target. In addition, as on-target editing...Reference Ma, Hong, et al. "Correction of a pathogenic gene mutation in human embryos." Nature. Epub 02 August...hypertrophic cardiomyopathy and other genetic disorders, pre-implantation genetic diagnosis (PGD) screens embryos...PubMed PMID: 28783728. Additional Resources on the Addgene Blog Check out Our New CRISPR Topic Page Read ...

  7. CRISPRainbow and Genome Visualization

    Type
    Blog Post
    Published
    Feb. 28, 2017, 3:30 p.m.
    ...detect gene copy number based on the number of fluorescent puncta observed. By labeling two genes of a ...vivo. Catalytically dead Cas9 (dCas9) cannot induce gene editing, but it retains its gRNA-directed targeting...Chen et al. successfully labeled protein-coding genes with both intronic and exonic gRNAs. In fact, the...also monitor the spatial relationship of the two genes over time, at a range of distances from 2-75 Mbp... envision using CRISPRainbow in combination with gene editing. CRISPRainbow requires very short, 11-mer...plasmids from this publication at Addgene. Additional Resources on the Addgene Blog Read More about Fluorescent...cells! This technique was developed in the lab of Addgene depositor Stanley Qi and used in Chen et al. to...

  8. Pairing CombiGEM and CRISPR for Combinatorial Genetic Screening

    Type
    Blog Post
    Published
    April 12, 2016, 2:30 p.m.
    ...functionally characterize combinatorial gene knockouts generated via multiplexed gRNA expression (Wong ...et al., 2014; Konermann et al., 2014) individual genes for studies interrogating their functions. The continual...list of effective gRNA sequences targeting your genes of interest. Thanks to the tremendous efforts made... et al. "Genome-scale CRISPR-mediated control of gene repression and activation." Cell 159.3 (2014): 647... "High-Resolution CRISPR Screens Reveal Fitness Genes and Genotype-Specific Cancer Liabilities." Cell ...Hongming, et al. "A CRISPR-based screen identifies genes essential for West-Nile-virus-induced cell death...incredibly useful for the efficient generation of individual (and multiple) genetic perturbations in single cells...

  9. Teaching an Old DOG New Tricks: Controlling Protein Activity with GFP

    Type
    Blog Post
    Published
    Nov. 24, 2015, 3:30 p.m.
    ...label cells. If GFP could be co-opted to control gene expression, he could then selectively manipulate...used LexA and rTetR systems such that they activate gene expression from different promoters. Moving to an.... GFP expression robustly activated the reporter gene TdTomato, whose expression was absent without electroporated...sites. Placing a lox-STOP-lox cassette upstream of a gene of interest blocks transcription when Cre is absent...present, it removes the STOP cassette, activating gene expression. Although Cre isn’t a modular protein...fluorescent proteins as scaffolds for cell-specific gene manipulation.” Cell 154(4) (2013): 928-939. PubMed...commonly used in optogenetics, opening up the possibility of adapting GFP lines for optogenetics experiments...

  10. Multicolor Animals: Using Fluorescent Proteins to Understand Single Cell Behavior

    Type
    Blog Post
    Published
    March 5, 2019, 1:08 p.m.
    ...deletion of one or several of these genes permits the expression of genes farther downstream in the construct... two tandem sets of genes aligned head-to-head and the deletion of one of the gene pairs. Brainbow 2.0...Brainbow uses a Cre/lox system to label neurons. Genes encoding the fluorescent proteins are organized ...organized in tandem with a single promoter, but only the gene directly following the promoter is expressed. Cre-mediated... which uses Cre to trigger the inversion of two genes aligned head-to-head. Another variation, Brainbow...located directly upstream of the fluorescent protein genes, Cre recombination is required before fluorescence...fluorescent proteins topic page Resources on Addgene.org Browse Addgene’s fluorescent protein collection Learn...

  11. Five Popular Model Organisms, Part 2

    Type
    Blog Post
    Published
    Sept. 29, 2020, 1:15 p.m.
    ...human genes have counterparts in the rat (Gibbs, et al., 2004). Additionally, breakthroughs in gene modification...abundance of genes related to limb regeneration (Vieira et al., 2019). To study regeneration scientists ...during and after embryogenesis. Addgene has over 600 plasmids that contain Xenopus genes and a tool-kit ...models.  Check out Addgene’s collection of plasmids containing Rattus norvegicus genes! Mexican axolotl ...array of molecular tools, such as gene manipulation and mutagenesis techniques, are available to the scientific...world worked to characterize each gene’s function. Some key genetics research with Arabidopsis include... for scientists to study human disease-relevant genes in such a controlled environment displays how essential...

  12. Plasmids 101: Restriction Cloning

    Type
    Blog Post
    Published
    Feb. 18, 2016, 3:42 p.m.
    ...you were cloning a gene into an expression vector, you would want the start of the gene to end up just downstream... 5’ of the start of the gene and a HindIII site just 3’ of the end of the gene. This way you can then ...leaving the 5’ end of your gene just downstream of the promoter and placing the gene in the proper orientation... multiple sites allows you to easily orient your gene insert with respect to the promoter. For example...restriction sites EcoRI, XhoI, and HindIII. To place your gene in the proper orientation downstream of the promoter...binds the plasmid to the new fragment thereby generating a complete, circular plasmid that can be easily...green, insert) are cut with restriction enzymes to generate compatible overhangs that allow them to bind. ...

  13. Your Top Requested Plasmid in 2016!

    Type
    Blog Post
    Published
    Jan. 11, 2017, 8:47 p.m.
    ...the puromycin resistance gene through a 2A peptide. The puromycin resistance gene enables selection of cells...to translate Cas9-2A and the Puromycin resistance gene as separate peptides. PX459 This is the vector backbone...had a point mutation in the puromycin resistance gene that made it less effective in certain cell lines...Resources on the Addgene Blog Browse Our CRISPR Posts Learn about Using CRISPR to Generate Knockouts Read...Purpose  SpCas9 This plasmid expresses Streptococcus pyogenes (Sp) Cas9 from a Cbh promoter. Cbh is a modified... the key component of most CRISPR systems and generates double strange breaks at target sites 5' of the...your 20 nucleotide guide sequence of choice (for generation of a gRNA that will target SpCas9 to the approrpriate...

  14. Hot Plasmids - June 2020 - Barcoded CRISPR Library, Sparse Cell Labeling, Calcineurin Reporter, and DNA Staining Dye Alternative

    Type
    Blog Post
    Published
    June 2, 2020, 1:15 p.m.
    ...the sgRNAiBAR library covers every annotated human gene, it enables genome-wide screening with a high MOI...a stop cassette upstream of an effector/reporter gene. In cells where the stop cassette is removed, the...fish, and mice, by replacing the effector with a gene of interest in the cells you are studying. Read ...calcineurin-dependent response elements (CDREs) and drives gene expression. Because calcineurin is required for ...antifungals that target calcineurin. Current calcineurin gene reporters are based on the bacterial βgal assay,...Competition (SPARC) is a fully genetic tool for labeling just a fraction of a genetically-defined subset of cells...lentiviral vectors the iBARs can be detected by next generation sequencing.  In order to avoid a high false-positive...

  15. Reaching out to China: Canton Nucleic Acids Forum (CNAF) 2015

    Type
    Blog Post
    Published
    Dec. 1, 2015, 3:30 p.m.
    ...sensitizing gene cassettes. Thse vectors are engineered to replace the drug resistantance genes in the bacteria...were identified for 61 such diseases. Now single gene alleles have been identified for 4,163, over 50%...Lowe talked about his work on the 500+ human tRNA genes and, until now, we've overlooked regulatory fragments...may be involved in preventing silencing of self-genes. Dr. Mello presented a hypothesis that these pathways...involved in RNA based surveillance of repetitive gene families and provide a way to guide inheritance ... field. Resources at Addgene Browse Addgene's RNAi resources Browse Addgene's CRISPR resources Read ...countries in number of plasmid requests from Addgene. Addgene - Canton Nucleic Acids Forum 2015 from Joanne...

  16. pCXLE toolkit: Efficient episomal plasmid-based method to reprogram peripheral blood cells to iPSCs

    Type
    Blog Post
    Published
    Dec. 14, 2017, 2:08 p.m.
    ...Ehrhardt, A., et al., Episomal vectors for gene therapy. Curr Gene Ther, 2008. 8(3): p. 147-61. PubMed PMID... functional genes at the site of insertion and which can potentially promote tumorigenesis in vivo. The...fellow at Massachusetts General Hospital with research interests in the fields of gene editing and stem cells...non-integrating plasmid from blood cells with unique epigenetic and gene expression signatures. Cell Res, 2011. 21...The phenomenon has been recorded in past gene therapy trials and remains a safety concern in the clinical...clonal T cell proliferation in two patients after gene therapy for SCID-X1. Science, 2003. 302(5644): p...the chances of insertional mutagenesis, which is the integration of transgenes into the genome that can ...

  17. CRISPR 101: Anti-CRISPR Proteins Switch Off CRISPR-Cas Systems

    Type
    Blog Post
    Published
    July 23, 2020, 1:20 p.m.
    ...used for genome editing, activating gene expression, repressing gene expression, and much more. But there...engineering viruses. For example, an Acr gene was used to replace genes in the difficult-to-engineer Sulfolobus... Selection marker while engineering viruses Acr genes can be used as positive selectable markers in engineering...et al., 2018). Only viral particles that have the gene deletion could replicate when challenged with the...Experiment Read more about Optogenetic Control of CRISPR   Resources on Addgene.org Find CRISPR Plasmids ...Nme) (Pawluk et al., 2016). While Streptococcus pyogenes Cas9 (SpyCas9) is the most used and well studied...10.1126/sciadv.1701620 Additional Resources on the Addgene Blog First time CRISPR user? Check out this post...

  18. A New Generation of Adenine Base Editors Improves Editing in Primary Human Cells

    Type
    Blog Post
    Published
    May 7, 2020, 1:15 p.m.
    ...editing single genes is very useful, sometimes, you need to modify multiple genes to generate a cellular ...Nicole Gaudelli, the Associate Director and Head of Gene Editing Technology at Beam Therapeutics, used a ...In addition to stem cells the authors targeted 6 genes in primary human T cells using 8 selected ABE8s ...the reduction in protein expression of these six genes. All ABE8s edited more efficiently than ABE7.10 ... was the winner. It could simultaneously edit 3 genes (98.1%, 98.3%, or 98.6% efficiency, respectively... eighth generation of adenine base editors, called ABE8 (Gaudelli et al., 2020). The generated ABE8s needed...replaced S. pyogenes Cas9 in the ABE complex with PAM-variant Cas9 proteins: the S. pyogenes NG-Cas9 (PAM...

  19. Anatomy of a Plasmid Page at Addgene

    Type
    Blog Post
    Published
    Feb. 4, 2016, 3:30 p.m.
    ...within the gene/insert, this information can be found here. There may be more than one gene inserted into...can easily clone my gene into it? Your best bet is the Plasmid Map: We auto-generate a plasmid map based... deposited with Addgene, you can list the original vector’s plasmid number here. Gene/insert: This describes...the backbone, and provides information about this gene or DNA element of interest. If the depositor has... into a backbone, and each is given its own gene/insert section on the plasmid page. Tags are listed according...We Do Quality Control at Addgene Resources on Addgene.org Join the Addgene Community Learn the Benefits...plasmid. Use the Addgene catalog number to easily find template citations for Addgene plasmids you have...

  20. Plasmids 101: Transformation, Transduction, Bacterial Conjugation, and Transfection

    Type
    Blog Post
    Published
    June 25, 2019, 12:54 p.m.
    ...Horizontal gene transfer (HGT) is the movement of genetic material between organisms. It plays a key ... comic, when a bacterium uptakes a plasmid, the genes on the plasmid can give them different phenotypes...this plasmid encodes a green fluorescent protein gene which makes the bacterium fluoresce green. Comic...used to create both transient cell lines, where a gene of interest is expressed but not integrated into...Conjugation was the first extensively studied method of gene transfer and was discovered in 1946 by Joshua Lederberg...uptake of genetic material from the environment by bacterial cells. In nature, this genetic material often...the donor can transfer genetic material to the recipient bacterium. The genetic material transferred is...

  21. Hot Plasmids - March 2020 - base editors, GEVI, MoClo, and optogenetics

    Type
    Blog Post
    Published
    March 3, 2020, 2:15 p.m.
    ...limits the delivery of larger transgenes, such as base editors which have a gene size of ~5.2 kb. To overcome...Article contributed by Jennifer Tsang AAVs are a great gene therapy delivery mechanism, but their small packaging...dosage comparable to those currently used in human gene therapy trials. They further tested the base editors...expression levels from different promoters, and inhibited gene expression using CRISPRi. The kit functions in the...that increases temporal and spatial precision of gene expression and reduces toxicity as compared to previous...fluorescent genetically encoded voltage indicator Article contributed by Shreya Vedantam Genetically encoded...splitting removes any exogenous sequences and regenerates a native peptide bond at the split site. ...

  22. Screening for Successful Genome Editing with Digital PCR

    Type
    Blog Post
    Published
    March 30, 2017, 2:30 p.m.
    ...NHEJ to generate translational reading frame-altering indels for “functional knockout” of a gene of interest...) validate successful mutation of your favourite gene, there are several different methods available. ... for complete functional knockout of your target gene. Mismatch nuclease assays are completely “blind”...U., Hauber, I. & Fehse, B. Digital PCR to assess gene-editing frequencies (GEF-dPCR) mediated by designer...et al. Digital detection of endonuclease mediated gene disruption in the HIV provirus. Sci. Rep. 6, 20064...validation, such as this "CRISPR 101" post. Next-generation sequencing technologies are the gold standard.... 6. Fu, Y. et al. High-frequency off-target mutagenesis induced by CRISPR-Cas nucleases in human cells...

  23. Viral Vectors 101: Types of viruses

    Type
    Blog Post
    Published
    June 6, 2023, 1:15 p.m.
    ... et al. Adenovirus-Mediated Gene Delivery: Potential Applications for Gene and Cell-Based Therapies in...Well, they are essentially evolutionarily optimized gene delivery devices. Their survival depends on their...Altogether, AAV has many excellent features for human gene therapy and is currently being used in clinical ...in the New Era of Personalized Medicine. Genes Dis. 2017 Jun;4(2):43-63. DOI: 10.1016/j.gendis.2017.04.001...v13102073 Resources on Addgene.org Adeno-associated Virus (AAV) Guide Addgene’s viral plasmids and resources...resources Addgene’s viral services: AAV Resources on the Addgene blog Viral Vectors 101: Viral Vector...due to their propensity to integrate into proto-oncogenes and disrupt their expression (Robinson, 1982)....

  24. Hot Plasmids - August 2020

    Type
    Blog Post
    Published
    Aug. 18, 2020, 1:15 p.m.
    ...stem cells By Jennifer Tsang CRISPR gene interference (CRISPRi) and gene activation (CRISPRa) are great ways...the plasmids at Addgene! A new tool for synthetic biology uses CRISPRi to control gene expression. This...nucleus and modulate transcription of the mCitrine gene. mCherry is constitutively expressed. Cells are ...selectivity and found that the best motif was from the Lck gene. For marking microtubules of mammalian cells, the... ways to modulate the expression of nearly any gene via specific guide RNA (gRNA) sequences. But these...-IgG1-Fc, Addgene #140700) pMAK463 (NBαMouse-IgK, Addgene #140701) pMAK464 (NBαEGFP, Addgene #140702) ...Find these AAVs at Addgene New controls, biosensor, chemogenetic and optogenetic AAV for expression ...

  25. Fluorescent Proteins 101: Fluorescent Protein Timers

    Type
    Blog Post
    Published
    May 4, 2017, 2:30 p.m.
    ...expression time. 2. Timer functionality with a single gene Although it is possible to make a timer system composed...cargoes to FP timer mk-GO (Tsuboi et al.) Monitor gene expression in the developing pancreas by placing...FP timer DsRed-E5 under the control of Neurog3 (a gene controlling pancreatic differentiation) in mouse...(trafficking to a subcellular location, start of gene expression, development of a cell structure, etc...formation of limbs are correlated with changes in gene expression. In a developing embryo, for example,...long-lived transcription factors control gross changes in gene expression as new body sections are formed. In synthetic...knowledge of protein structure and saturation mutagenesis. mK-GO matures from green to red, and the FT ...

  26. CRISPR 101: Off-Target Effects

    Type
    Blog Post
    Published
    Feb. 8, 2024, 2:15 p.m.
    ...confounding mutations, the risk is high. You are generating a gene therapy product with CRISPR to (hopefully)...not to cleave DNA and instead activates/represses gene expression by various mechanisms. The RNA guide ...which involves making specific knock-out edits in a gene(s) of interest and has an average off-target frequency...involves making a large sgRNA library to thousands of genes and targeting and sequencing millions of cells. ...confound an experiment due to their location (e.g. in a gene in a similar pathway, a growth factor, etc.) this...Increasing the Specificity of the CRISPR-Cas System in Gene Editing. Mol Ther. 2021 Mar 3;29(3):937-948. doi... Gao F, Guo Y. Off-target effects in CRISPR/Cas9 gene editing. Front Bioeng Biotechnol. 2023 Mar 9;11:...

  27. Multiplex Genome Editing with CRISPR-Cpf1

    Type
    Blog Post
    Published
    May 9, 2017, 2:12 p.m.
    ...Cpf1 multiplexing method, other multiplex CRISPR gene editing methods relied solely on Cas9. Overall, ...Lastly, an AAV vector was used to multiplex edit 3-4 genes in a primary culture of mouse neurons and in vivo...summary of multiplexing efficiency in vivo. Multiplex gene editing with CRISPR-Cpf1 is one of the latest developments...transfection, viral transduction) for simultaneous multi-gene editing with a single crRNA array. Are you interested...        References 1. Zetsche et al. "Multiplex Gene Editing by CRISPR–Cpf1 Asing a Single crRNA Array...review of Cpf1, check out this blog post or see Addgene's CRISPR guide page for a review of Cas9.  For a...plasmids; for multiple edits, single plasmids are generally more efficient. Pro-Tip Make sure to reverse the...

  28. Tips for Titering Your Lentiviral Preps

    Type
    Blog Post
    Published
    March 15, 2016, 2:30 p.m.
    ...LTRs, gag, WPRE, antibiotic resistance-genes, or the transgene itself. Many researchers prefer to design...multiplicity of infection (MOI) for predicting gene transfer events." Genetic vaccines and therapy 2.1 (2004): 1....with your virus and assaying for expression of a gene carried on the transfer plasmid or quantifying the...popular approach utilizes the antibiotic resistance genes carried on transfer plasmids. Target cells are transduced... to contain 1 integrated copy of the qPCR target gene. Developing the proper control line typically requires...Additional Resources on Addgene.org Learn How We Do Viral Production at Addgene Read Our Lentiviral Guide... that specific feature. Primers targeting the transgene are also beneficial as they ensure that the correct...

  29. Comparing Cas9 to NgAgo: Can the Argonautes Best CRISPR?

    Type
    Blog Post
    Published
    June 9, 2016, 2:30 p.m.
    ... certain guide sequences - using 5 guides/gene for 10 genes, they did not observe differences in cleavage... BELOW Biologists are going gaga over the newest gene-editing protein - a DNA-cleaving Argonaute from ... original sequence. If you’re looking to disrupt gene function via indel formation, this property could...Natronobacterium gregoryi, or NgAgo for short. Addgene has already distributed this plasmid all over the...supplied. Find Plasmids from the NgAgo Publication at Addgene Cas9 and NgAgo head to head NgAgo has a number ...27136078. Find plasmids from this publication at Addgene. 2. Swarts, Daan C., et al.  “DNA-guided DNA interference...PMC4697943. Find plasmids from this publication at Addgene. 3. Swarts, Daan C., et al. “Argonaute of the ...

  30. Ghanaian Researchers to Receive Plasmid Awards from Addgene and Seeding Labs

    Type
    Blog Post
    Published
    June 15, 2016, 1:30 p.m.
    ...developed as well as CRISPR-based gene repression tools to find and test genes that may play a role in the ...proteomic tools to discover genes and proteins associated with pathogenesis. In his future work. Dr. Bengyella...in China Additional Resources on Addgene.org Learn About Addgene's Mission Check Out Our International...As we mentioned a few months ago, Addgene has been working with Seeding Labs to provide plasmid grants...and Duedu and how they plan to use plasmids from Addgene to advance their research. Louis Bengyella Dr. ...that enable E. coli and Citrobacter freundii to generate sugars from cellulosic biomass. Dr. Duedu would...would like to use the plasmids he acquires through Addgene to produce molecular biological tools like Taq ...

  31. Hot Plasmids: Spring 2025

    Type
    Blog Post
    Published
    June 3, 2025, 1:15 p.m.
    ... Strong expression: A CMV promoter drives robust gene expression in mammalian cells. Flexible tagging:...tag. Selectable and stable: A puromycin resistance gene makes stable cell line creation a breeze. Modular...labeling By Alyssa Shepard To fully understand how genes are regulated, it's important to know what proteins...identify both new and known regulators at the FOS gene promoter and the MYC locus (Figure 5). Figure...identification of heat-shock proteins at the FOS gene promoter using TurboCas, streptavidin immunoprecipitation...very quick and efficient way to learn more about gene regulation. Find the TurboCas plasmid here! Cenik...Enhancer-AAVs targeting interneurons By Mike Lacy Targeting gene expression to specific cell types in the brain can...

  32. The MTA at Addgene

    Type
    Blog Post
    Published
    July 23, 2024, 2:17 p.m.
    ...MTAs per year.  The MTA Process at Addgene As a repository, Addgene doesn’t actually own the majority ...distribution for their plasmids. MTAs at Addgene Since Addgene was founded in 2004, we’ve handled the majority...If you’ve deposited or requested plasmids from Addgene, then you’ve been involved in the material transfer.... The UBMTA, which is the MTA most plasmids at Addgene are shared under, is well-understood and accepted...for managing research use agreements like MTAs.  Addgene does a lot to ensure that we can facilitate a fast...signing of MTAs by the Provider and Recipient (in Addgene parlance, the depositor and requestor) organizations...that differs.  When you deposit a plasmid with Addgene, your organization’s tech transfer office is sent...

  33. Bioinformatics at Addgene

    Type
    Blog Post
    Published
    July 14, 2022, 1:15 p.m.
    .... More than half of these sequences were generated by Addgene as part of our QC process. Automation and...From the beginning, one of Addgene’s goals was to ensure the scientific community obtained high-quality...the years to keep up with the latest tools. In Addgene’s early days, we would ‘spot-check’ critical regions...sequencing at our facility using Illumina MiSeq Next Generation Sequencing (NGS) technology. As our repository...will describe the importance of bioinformatics at Addgene and how our search for solutions led us to a partnership...bioinformatics needs       Fig. 1: QC'ing data at Addgene is like standing under a Niagara Falls sized rainbow...our software, even with continuous updates. The generous donation of a Illumina MiSeq sequencer enabled...

  34. Working 9-5: an Addgene Co-op

    Type
    Blog Post
    Published
    June 2, 2022, 2:15 p.m.
    ...intended. With the use of applications like SnapGene and Geneious, I was able to determine if any discrepancies...able to explore other parts of Addgene. This was done through the “Addgene 101” series, where each team ... Communication function at Addgene. Working a 9-5 As it turned out, Addgene uses something called “core...completing their co-op at Addgene. This is her second contribution to the Addgene blog.      More resources...brought me, a second year bioengineering student, to Addgene. I am currently working on the QC/QA team as a ...specific overview. It was immediately clear that Addgene wanted to ensure applicants understood what the...that the two hours were split up between several Addgene employees, who all made the daunting task feel ...

  35. Addgene at SfN23!

    Type
    Blog Post
    Published
    Nov. 9, 2023, 2:15 p.m.
    ... at Addgene's booth, #3422, in the nonprofits section.  The Booth What will you find at Addgene's booth...scientific curation manager, Angela, experts on the Addgene repository, are also attending! Finally, our team....  You'll also find tons of information about Addgene's ready-to-use antibodies, viral vectors, and, of...course, plasmids! Plus, we'll be giving away fun Addgene swag for the duration of the conference (be warned...

  36. Viral Vectors 101: Pseudotyping

    Type
    Blog Post
    Published
    April 20, 2021, 2:30 p.m.
    ...Precious Tools to Improve Gene Modification of Hematopoietic Cells for Research and Gene Therapy. Viruses 12...To deliver genes using lentiviral vectors, you need an envelope protein on the virus’s surface and a ... plasmid which contains a promoter, the envelope gene, and a polyA tail.   Figure 1: Lentiviral...can’t be used for neuronal tracing or peripheral gene delivery. Combining the rabies glycoprotein into...Lentiviral Vectors: One Vector, Many Guises. Human Gene Therapy Methods 28:291–301 . https://doi.org/10.1089...Resources on Addgene.org: Check out the lentivirus guide Find ready-to-use lentiviral preps at Addgene ...your viral vector, you use the same protocol for generating lentivirus, but you use a different envelope ...

  37. Getting the Most from Your Lentiviral Transduction

    Type
    Blog Post
    Published
    April 7, 2016, 2:30 p.m.
    ...cell lines that stably express a gene of interest. While the general approach for using lentivirus, infect...multiplicity of infection (MOI) for predicting gene transfer events.” Genetic Vaccines and Therapy 2.1 (2004) Pubmed...Mycoplasma infection significantly alters microarray gene expression profiles.” Biotechniques 35.4 (2003)....Downstream processing of oncoretroviral and lentiviral gene therapy vectors.” Biotechnology Advances 24.3 (2006...production of lentiviral and retroviral vectors.” Human Gene Therapy Clinical Development 25.3 (2014). 175-85...lentiviral vectors through pseudotyping.” Current Gene Therapy 5.4 (2005). 387-98. Pubmed PMID: 16101513... cells do not take up nucleic acid or express transgenes as well as actively dividing cells resulting ...

  38. Your Top Requested Plasmid in 2015!

    Type
    Blog Post
    Published
    Dec. 30, 2015, 3:30 p.m.
    ...to knock-out single genes in clonal populations and to knock-out thousands of genes in mixed population...targeting over 19,000 and 20,000 human and mouse genes respectively, use lentiCRISPR v2 as the backbone...Resources on the Addgene Blog Browse Our CRISPR Posts Learn about Using CRISPR to Generate Knockouts Read...announce that the plasmid most requested from the Addgene repository in 2015 was... lentiCRISPR V2   LentiCRISPR...Availability and Use LentiCRISPR v2 is available from Addgene both as a single empty vector and in pooled libraries...reagents available to the research community through Addgene and look forward to another great year of sharing... Plasmids to Mammalian Cells Resources on the Addgene Website Visit our Lentiviral Guide Pages Find ...

  39. CRISPR in the Clinic

    Type
    Blog Post
    Published
    Nov. 2, 2023, 1:15 p.m.
    ... J.A. Engineering the next-generation of CAR T-cells with CRISPR-Cas9 gene editing. Mol Cancer 21, 78 ...dominant cataract disorder caused by a single copy of a gene known as Crygc (Wu et al). They found that the cataracts...able to demonstrate functional correction of the gene in clonally expanded organoids. This work, and many...immunotherapy for cancer treatment by traditional gene therapy pipelines (viral), and researchers are currently...single nucleotide polymorphisms (SNPs) in the TTR gene and is molecularly characterized by misfolding of...blog post on CRISPR’s potential for correcting monogenetic diseases. Now, almost 10 years later, CRISPR’...Resources References Yuxuan Wu et al. "Correction of a Genetic Disease in Mouse Via Use of CRISPR/Cas9." Cell ...

  40. Adapting Toehold Switches to Detect Zika Virus

    Type
    Blog Post
    Published
    Aug. 30, 2016, 2:30 p.m.
    ... at Addgene 2. Green, Alexander A., et al. "Toehold switches: de-novo-designed regulators of gene expression...was an in vitro paper-based platform that allowed gene circuits to be deployed out of the laboratory in...combined technologies, we built paper-based synthetic gene networks for the detection of antibiotic resistance...-of-concept work was exciting because it enabled gene circuit-based sensors to be used outside of the ... 3. Pardee, Keith, et al. "Paper-based synthetic gene networks." Cell 159.4 (2014): 940-954. PubMed PMID...Sensor Constructs at Addgene As we report, we have embedded this next generation of toehold switch-based...corresponding portions of the Zika virus genome with Addgene to facilitate rapid sharing of these tools with...
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