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  2. Build Your CRISPR Vocabulary

    Type
    Blog Post
    Published
    July 2, 2024, 1:15 p.m.
    ...other hand, viruses can evolve to evade CRISPR by mutating these sequences. Pro tip! Various labs have created...

  3. Hot Plasmids and Viral Preps - July 2021

    Type
    Blog Post
    Published
    July 27, 2021, 1:15 p.m.
    ...control and a SL-sensitive fluorophore (circularly permuted green fluorescent protein or cpGFP) to the petunia...

  5. Hot Plasmids - October 2022

    Type
    Blog Post
    Published
    Oct. 6, 2022, 1:15 p.m.
    ...high-throughput screening platform to apply saturating mutagenesis on 21 positions in the protein sequence of the...

  10. 10 Great Guest Posts We're Thankful For

    Type
    Blog Post
    Published
    Nov. 18, 2016, 3 p.m.
    ...fuel sources.” In her work, Dr. Polka made R-body mutants that respond to different pH’s and some that are...

  13. Antibodies 101: Epitope Tags

    Type
    Blog Post
    Published
    May 11, 2023, 1:15 p.m.
    ...Tags can enable the separation of detection of a mutant protein vs. endogenous WT levels of the same protein...

  14. New Viral Vectors - Winter 2025

    Type
    Blog Post
    Published
    Jan. 28, 2025, noon
    ...viral prep with multiple serotypes pAAV-hSyn-GRAB_OTmut AAV1 Biosensor Yulong Li New viral prep pAAV-CAG-NIR-GECO2G...

  16. Plasmid Cloning by PCR

    Type
    Blog Post
    Published
    March 29, 2016, 2:30 p.m.
    ...to use a high fidelity polymerase to minimize mutations. The fidelity of the polymerase becomes more important...

  18. Science Career Options

    Type
    Blog Post
    Published
    Feb. 23, 2017, 1:30 p.m.
    ...will you stay in one location? How long is the commute? Will the job require public speaking? Will you...

  20. DAP arrays for multiplex gene editing

    Type
    Blog Post
    Published
    July 7, 2022, 1:15 p.m.
    ...any CRISPR-based gene editors, for correcting mutations or installing functional genes to study and potentially...

  23. Components of CRISPR/Cas9

    Type
    Blog Post
    Published
    Feb. 2, 2016, 5 p.m.
    ...Cas9 homologs from different organisms or Cas9 mutants developed in a variety of labs (see table below...

  25. Luminescent Imaging with Nano-lanterns

    Type
    Blog Post
    Published
    May 25, 2017, 2:30 p.m.
    ...described the first Nano-lantern, a fusion of a mutagenized Renilla luciferase (RLuc8) with Venus. This luminescent...

  26. Top Requested Lentivirus and AAV of 2016

    Type
    Blog Post
    Published
    Jan. 6, 2017, 3:56 p.m.
    ...transduction with this virus. While various Cas9 mutants have been engineered to perform unique editing ...

  29. Viral Vectors 101: Viral Applications

    Type
    Blog Post
    Published
    April 13, 2023, 1:15 p.m.
    ... have been engineered to introduce frameshift mutations in genes of interest, turning expression off permanently...

  30. Viral Vectors 101: Biosensors

    Type
    Blog Post
    Published
    July 6, 2023, 1 p.m.
    ...and are ready-to-use. While biosensors span the gamut of applications within chemistry, biology, and physics...

  36. Antibodies 101: Affinity Reagents

    Type
    Blog Post
    Published
    April 14, 2022, 1:15 p.m.
    ...fragments. However, rigidity can be introduced through mutation, allowing diabodies to be used for assembling ...

  38. All in a Twist: dsRNA

    Type
    Blog Post
    Published
    Feb. 2, 2023, 2:15 p.m.
    ...inflammatory response. However, if dsRNA sensors are mutated or mechanisms which suppress endogenous dsRNA formation...

  39. CRISPR 101: Cas9 vs. The Other Cas(s)

    Type
    Blog Post
    Published
    Dec. 8, 2022, 2:15 p.m.
    ...engineering goals expand beyond generating frameshift mutations or single base pair edits, these enzymes are worth...
Showing: 201 - 240 of 312 results