We narrowed to 318 results for: Mut

...activation and genome editing by fusing Cas9 to a mutated human estrogen receptor. This protein fusion stays...

...useful to me as some days it was not as easy to commute as others. I know some fear that remote work would...

...manager on paper or as an email send directly from a mutual connection. If the organization is small, any person...

...other hand, viruses can evolve to evade CRISPR by mutating these sequences. Pro tip! Various labs have created...

...to generate a collection of monitored genes and mutants - viral integration, transposon, and many more!...

...confirm the identity of the gene and check for any mutations. Once we identify the gene, we can easily annotate...

... The opsin ChETA, for example, is a ChR2 opsin mutated to have faster off kinetics and allow neurons to...

...control and a SL-sensitive fluorophore (circularly permuted green fluorescent protein or cpGFP) to the petunia...

...high-throughput screening platform to apply saturating mutagenesis on 21 positions in the protein sequence of the...

... exciting tool for producing specific genetic mutations and gene disruptions without the use of viral ...

...CRISPR/Cas defense by generating a single point mutation in the PAM sequence, preventing the Cas nucleases...

...sgRNAs guide Cas9 to cut targeted genes, creating mutations that prevent their expression. The knockout cells...

...viral prep with multiple serotypes pAAV-hSyn-GRAB_OTmut AAV1 Biosensor Yulong Li New viral prep pAAV-CAG-NIR-GECO2G...

...Tags can enable the separation of detection of a mutant protein vs. endogenous WT levels of the same protein...

...Kwart, including a detailed look at how cut-to-mutation distance affects editing efficiency. Optimizing...

...receptor and subsequently evaluated interactions with mutant and wild-type erythropoietin molecules. The in ...

...modify up to 60% of the disease-causing point mutations. They allow single-nucleotide modifications to...

...cloning strains, browse the table of common gene mutations in our Plasmids 101: Common Lab E. coli Strains...

...fuel sources.” In her work, Dr. Polka made R-body mutants that respond to different pH’s and some that are...

... Cas9 Homolog Cpf1 Check Out High Fidelty Cas9 Mutants Browse All Genome Engineering Posts Additional...

...to use a high fidelity polymerase to minimize mutations. The fidelity of the polymerase becomes more important...

...modeling, directed evolution, site-saturation mutagenesis, and medium-throughput screening, she increased...

...can’t really help you. The exception is having a mutual acquaintance connect you to someone who you need...

...later optimized these first generation FLiPs by mutating Dronpa to lower its aggregation tendency, increase...

...any CRISPR-based gene editors, for correcting mutations or installing functional genes to study and potentially...

...will you stay in one location? How long is the commute? Will the job require public speaking? Will you...

...if breaking the gene is worse than leaving the mutation alone). CRISPR delivery by nucleofection The in...

...Cas9 homologs from different organisms or Cas9 mutants developed in a variety of labs (see table below...

...Prathima has a research background in protein mutagenesis and cancer immunotherapies. She is currently ...

...and are ready-to-use. While biosensors span the gamut of applications within chemistry, biology, and physics...

...essentially those needed to make site-directed mutagenesis, many researchers new to GCE aren’t able to successfully...

... have been engineered to introduce frameshift mutations in genes of interest, turning expression off permanently...

... contain the expected features and are free of mutation.  Once the samples arrive, each group of plates...

...immune measures and correlating them with tumor mutations and clinical responses. This platform has the ...

...described the first Nano-lantern, a fusion of a mutagenized Renilla luciferase (RLuc8) with Venus. This luminescent...

...transduction with this virus. While various Cas9 mutants have been engineered to perform unique editing ...

...essentially any gene,” she says. The technical leap from mutating and deactivating a gene or genes to selectively...

... gRNA is all you need to generate a frameshift mutation that will permanently ablate protein expression...

...inflammatory response. However, if dsRNA sensors are mutated or mechanisms which suppress endogenous dsRNA formation...

...early-onset cataracts, is characterized by point mutations in the Iron Response Element (IRE) RNA motif of...