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We narrowed to 602 results for: ACE

Showing: 251 - 300 of 602 results

  1. The Challenges of Cell Culture

    Type
    Blog Post
    Published
    May 5, 2016, 2:30 p.m.
    ...forensic technique (aka DNA fingerprinting) that can place suspects at the scene of a crime (every human in...experience through working for a variety of pharmaceutical companies including GSK, AstraZeneca-MedImmune...talks and seminars on cell culture and other pharmaceutical topics. The LGC Group also provides standards...

  2. Getting the Most from Your Lentiviral Transduction

    Type
    Blog Post
    Published
    April 7, 2016, 2:30 p.m.
    ...protein attaches to cell surface receptors, other important binding events take place during the course of...viral envelop and the receptors on the target cell surface. The possible interactions and hence possible targets...

  3. SARS-CoV-2/COVID-19 Detection Methods Based on CRISPR/Cas

    Type
    Blog Post
    Published
    May 5, 2020, 1:15 p.m.
    ...target site, while Cas12a requires a PAM (Protospacer Adjacent Motif) for target cleavage. This test can...assay. This reaction also contains SSB, strand displacement DNA polymerase, recombinase, and the ssDNA-FQ...

  5. Plasmids 101: Biotinylation

    Type
    Blog Post
    Published
    Nov. 15, 2018, 1:50 p.m.
    ...Immunosorbent Assay) Southern/Northern blotting Cell surface labelling Attaching polypeptides to a substrate...non-selectively label DNA and RNA. Photobiotin acetate consists of a biotin molecule attached to a photoreactive...activated patterning of dye-labeled molecules on surfaces.” Journal of the American Chemical Society 125.27...

  6. Targeting HIV-1 with CRISPR: Shock and Kill or Cut it Out?

    Type
    Blog Post
    Published
    May 10, 2016, 2:30 p.m.
    ...reservoir, researchers have previously used histone deacetylase (HDAC) inhibitors to increase transcription of... for an “escape” virus to propagate and infect adjacent cells. It will be important to determine if CRISPR...application can benefit many others and accelerate the pace of research. We at Addgene hope to see the problem...

  7. Scientific Reproducibility - Focusing on Solutions at the Minisymposium on Reproducibility

    Type
    Blog Post
    Published
    May 18, 2018, 7:32 p.m.
    ... characterised antibodies against all human extracellular proteins with community-based validation. They...experiments on similar subjects in a single, searchable space. Finally there are new publishing outlets like F1000...their experimental designs. There are many more facets to reproducibility issues in the biological sciences...

  8. Viral Vectors 101: AAV Variables That Matter

    Type
    Blog Post
    Published
    June 1, 2023, 1:15 p.m.
    ... Modification. Pharmaceutics, 13(5), 750. https://doi.org/10.3390/pharmaceutics13050750 Murlidharan, G...GABA neuron, for instance, may not transduce the adjacent norepinephrine neuron at all. There may be more...

  9. Antibodies 101: Multiplex Immunofluorescence

    Type
    Blog Post
    Published
    May 23, 2023, 1:15 p.m.
    ...you want to know if they co-localize at the same place in the cell? Or you know that the presence of several...your microscope. Abcam's sample protocol is a good place to get started.  One important consideration when...the offending red-green image, go to Image>Color>Replace Red with Magenta. Or you can use Color>Split Channels...

  10. Hot Plasmids: Spring 2025

    Type
    Blog Post
    Published
    June 3, 2025, 1:15 p.m.
    ...needed to replicate inside a host cell but with space to insert your genetic cargo of choice. After hearing...GAG), and are (typically) tethered to the cell surface by a GPI-anchor. Image courtesy of IPI.   ...(LAMP5) — as well as cholinergic (choline acetyltransferase, ChAT) neurons. The authors characterized ...

  11. Recombinase-based State Machines Enable Order-dependent Logic in vivo

    Type
    Blog Post
    Published
    July 28, 2016, 2:30 p.m.
    ...PBAD), and the A118 gene downstream of the diacetylphloroglucinol (DAPG)-inducible promoter (PPhlF).  Output...we used to drive recombinase expression may be replaced by sensors that correspond to the desired inputs...are composed. Fear not – the state machine design space is highly degenerate and often times there are multiple...

  12. Generating Mouse Models Using CRISPR/Cas9

    Type
    Blog Post
    Published
    July 12, 2016, 2:30 p.m.
    ...the run, it got “dirty”, with multiple sequence traces overlapping each other! When the count was done...nucleotide change, insertion of a reporter gene, or replacement of the murine sequence with a human gene. Advantages...comes to larger scale genome editing, such as replacement of a mouse gene with its human ortholog (greater...

  13. Plasmids 101: Restriction Cloning

    Type
    Blog Post
    Published
    Feb. 18, 2016, 3:42 p.m.
    ...restriction enzymes that allow your insert to be placed into the backbone in the proper orientation. For... restriction sites EcoRI, XhoI, and HindIII. To place your gene in the proper orientation downstream of... important to have nice crisp bands and to have space to cut out the bands. Because of this we recommend...

  14. Building Global Connections with the International Mentorship Program USA-EUROPE

    Type
    Blog Post
    Published
    Nov. 6, 2015, 3:30 p.m.
    ...of the world, professional internationalization faces several obstacles; for instance there are few resources...career are common problems that many others have faced yet, still, this challenge did not hinder them from...evaluation of the pilot program IMP 2014/2015, took place on July 27th, 2015 at a dedicated international ...

  15. Quick Guide to All Things Lentivirus

    Type
    Blog Post
    Published
    March 21, 2017, 2:30 p.m.
    ...packaging plasmid caused the Psi sequence to be placed on the packaging plasmid forming a vector that ... Alternatively, your gene and the marker can be placed under the control of two different promoters. Both... Tet-off system a Tet-response element (TRE) is placed upstream of the promoter in your transfer vector...

  16. Lambda Red: A Homologous Recombination-based Technique for Genetic Engineering

    Type
    Blog Post
    Published
    Dec. 15, 2016, 3:57 p.m.
    ...Overview of using Lambda Red recombineering system to replace a gene of interest with an antibiotic resistance...as non-selectable DNA fragments, such as gene replacements and tags.  The typical frequency of recombinants....e. make changes to the third base pair of the adjacent 4-5 codons that alter the nucleotide sequence ...

  17. Transferable Skills Guide: Teamwork

    Type
    Blog Post
    Published
    Nov. 28, 2017, 2 p.m.
    ...teamwork was what drew me to the school in the first place. The famous discoveries and experiments I’d been...at Addgene, an unofficial title that I happily embrace (and admittedly, I may have started that nickname...sometimes even necessary, to build a team in the first place? Let’s think about some of the advantages of team-based...

  18. A Guide to Getting Started in Undergrad Research

    Type
    Blog Post
    Published
    June 8, 2021, 1:15 p.m.
    ...that’s where you’re at too, it’s a perfectly great place to begin. Here’s my advice: Reflect on your personal... next four years. But college, and science, are places for learning and growth! As you learn more about....org/10.1126/science.aba8039 Cordova FA (2016) Embrace uncertainty. Science 351:994–994 . https://doi....

  19. With an Eye Towards the Future, We Look Back at the March for Science

    Type
    Blog Post
    Published
    Oct. 24, 2017, 1:54 p.m.
    ...government, evidence to inform policies, a protected place to work, funding for science, and numerous other...every political persuasion) must be humble in the face of evidence. Stephanie Hays is moving from engineering...Harvard University to studying an evolutionary arms race at University of California, Berkeley. She is a ...

  20. Viral Vectors 101: Systemic Capsids

    Type
    Blog Post
    Published
    Oct. 12, 2023, 1:15 p.m.
    ...ultrasound (FUS), intracorporeal (IC), intracerebroventricular (ICV), intramuscular (IM), intrathecal ...many of the same restrictions, like limited cargo space (~4.7 kb) for your genetic payload — there are a...enhancers, and switches all take up precious cargo space in AAV vectors. Dual expression systems, which use..., 2019). LY6A is functionally expressed on the surface of brain endothelial cells in C57BL/6J mice, but...

  21. Participate in the Pets of Addgene Scavenger Hunt

    Type
    Blog Post
    Published
    April 1, 2021, 1:15 p.m.
    ... year to give our lab and shipping teams enough space at HQ and for some of us, that means spending more...co-workers” have made their way onto our Slack workspace over the last year. Now, we’ve collected photos...

  22. Summer Fun at Addgene!

    Type
    Blog Post
    Published
    May 12, 2022, 1:15 p.m.
    ...Are you following us on Twitter or Instagram (or Facebook or LinkedIn) but want to hear and see even more...behind-the-scenes peaks of Addgene’s people, culture, and workplace. Rumor has it that a few Addgenies* may even be...

  23. 2018 #DeckTheLab Contest Winners

    Type
    Blog Post
    Published
    Jan. 15, 2019, 2:19 p.m.
    ...Matthew J. Sikora (@mjsikora) December 18, 2018 Fireplace from the Dyer Lab If you’re feeling as chilly ..., they turned one of the lab computers into a fireplace scene.  Got our @EmoryChem lab office looking...

  24. Grad School Advice Part 2: Building Community

    Type
    Blog Post
    Published
    Oct. 6, 2016, 2:30 p.m.
    ...to younger graduate students   Check Out Angela Depace's Talk on "Precision and Plasticity in Mentoring...studies developmental gene regulation with Dr. Angela DePace in the Department of Systems Biology, and he tweets...

  25. Inntags: Innovative Protein Epitope Tagging

    Type
    Blog Post
    Published
    Nov. 10, 2015, 3:30 p.m.
    ... tag a protein, inntags IT5 and IT6 are a great place to start. These tags are easy to use in multiple...high-throughput applications. Perhaps IT5 and IT6 will even replace our older epitope tags - only time will tell! ...

  26. Editor's Choice, September 2016

    Type
    Blog Post
    Published
    Oct. 7, 2016, 4 p.m.
    ...useful post was contributed by guest blogger Sean Stacey from Eastern Tennessee State University and provides..., Lianna Swanson, and researcher at Sanofi Pharmaceuticals. This post highlights a rare disease, Fibrodysplasia...

  27. Scientist Networking: What is an Informational Interview?

    Type
    Blog Post
    Published
    July 1, 2014, 3:42 p.m.
    ...Perhaps you can offer to buy coffee or lunch at a place convenient to your interviewee? It is always best... interaction. Most people like to help One good place to look for interesting connections is your university...

  29. Scientific Sharing in the Time of COVID-19: Databases and Resources

    Type
    Blog Post
    Published
    May 12, 2020, 1:15 p.m.
    ... to scientists around the world. To support this pace of research, new, centralized databases and centers...protocols, publications, and more to accelerate the pace of research into COVID-19. While this program is...

  31. New Norepinephrine Indicators: nLightG and nLightR

    Type
    Blog Post
    Published
    Dec. 12, 2023, 2:15 p.m.
    ...pixel-wise ΔF/F0 heatmaps. White insets indicate surface expression of the indicators over white dashed ...Wasielewski, L. M., Sönmez, L., Benke, D., Weber, B., Bohacek, J., Reiner, A., … Patriarchi, T. (2023). Sensitive...

  32. pSiM24: Simplifying Plant Genetic Engineering

    Type
    Blog Post
    Published
    Sept. 29, 2015, 2:30 p.m.
    ...efficiency in both types of bacteria. They also replaced the promoter regulating the gene of interest with...the M24 promoter, allowing it to be removed and replaced if needed. In total, three MCS regions permit ...

  33. 6 Steps to Submitting a Resume That Gets Seen

    Type
    Blog Post
    Published
    July 9, 2015, 2:30 p.m.
    ...and to get the important information in the right place on the page. 5) Apply for the job as requested in...Resources representative). Pro tip: if there is no place to upload a cover letter, make your cover letter...

  36. Genetically-encoded Sparse Cell Labeling - A SPARC of Innovation

    Type
    Blog Post
    Published
    May 21, 2020, 1:15 p.m.
    ...In the second-generation SPARC2 system, HDVR is placed upstream of the second attP site to cleave and ...expression of using the SPARC system. The effector is placed downstream of the rest of the SPARC components ...

  37. Sensing Neuronal Dopamine

    Type
    Blog Post
    Published
    July 30, 2019, 12:59 p.m.
    ...the brain has been in vivo microdialysis of extracellular fluid (Darvesh et al., 2011). This technique... across the semipermeable membrane from the extracellular fluid into the perfused liquid. The probe then...

  38. Addgene's a Nonprofit? Nonprofit Awareness Day 2017

    Type
    Blog Post
    Published
    June 5, 2017, 3:19 p.m.
    ...scientists to deposit and request plasmids (via web interface, processes, distribution, and customer service...Questions? Feel free to reach out to us on Twitter, Facebook, LinkedIn, email, or even the comments section...

  39. Bricking Science: Portraying Scientific Reality Through LEGO

    Type
    Blog Post
    Published
    Nov. 29, 2016, 3:30 p.m.
    ...of plastic for us now). With the personality in place, everything now is about the plot. This part is ...after all, who working in a laboratory has never faced an odd situation with experiments? Our art simply...

  40. New CRISPR Web Resources and #12DaysofCRISPR Recap

    Type
    Blog Post
    Published
    Dec. 12, 2017, 3:13 p.m.
    ...tools is especially exciting, including histone acetylation by p300, histone demethylation by LSD1, cytosine... REPAIR (RNA Editing for Programmable A to I Replacement) system. Like RNA targeting systems, RNA editing...

  41. 5 Uncommon Ways to Gain Career Skills During an Uncommon Time

    Type
    Blog Post
    Published
    Oct. 20, 2020, 1:15 p.m.
    ...level of intimacy that creates the magic of a safe space. No matter why, now is a great time to find a few...Funding Opportunities Open Education Database: 100 Places to Find Funding For Your Research University of...

  43. Meet the New Addgene Board Members!

    Type
    Blog Post
    Published
    April 28, 2020, 1:14 p.m.
    ...Therapeutics, which was acquired by Ultragenyx Pharmaceutical in 2017. As one of the original employees at...impactful. At Novartis, Stegmeier deposited the ClonTracer Barcoding Library, a library that “enables the...

  45. What Do I Do Now? Academic v. Non-Academic Career Decisions

    Type
    Blog Post
    Published
    March 9, 2017, 3:40 p.m.
    ...self-promoting—No one is going to tell you to pick up the pace Constant worry about grants and renewals What is... can be an issue in getting promoted (especially race and gender) Interpersonal issues can create interpersonal...

  48. In Vivo Biotinylation of Bacterial Fusion Proteins

    Type
    Blog Post
    Published
    Jan. 25, 2018, 2:09 p.m.
    ...successfully used this plasmid to biotinylate the extracellular domain of the erythropoietin receptor and subsequently...kinetics using a multitude of methods including surface plasmon resonance (Biacore, GE Life Sciences) or...

  50. Summer SciComm Series: Master of SciComm

    Type
    Blog Post
    Published
    July 21, 2022, 1:15 p.m.
    ...communication, then, is its sheer versatility in the face of disparate spheres of research. In my case, an...Over time, this interest came to incorporate other facets which occupied my attention, until these coalesced...
Showing: 251 - 300 of 602 results