Addgene

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Showing: 251 - 300 of 398 results

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Management for Scientists: What Makes a Good Manager Anyway?

Type

Blog Post

Published

Dec. 9, 2014, 2:22 p.m.

...manage their personal lives to achieve a healthy work-life balance. It is harder to manage when the measure...
10 Ways to Share Your Science!

Type

Blog Post

Published

May 13, 2014, 8:34 p.m.

...Science Communication Program at the University of California, Santa Cruz, has been turning scientists into...
Plasmids 101: Antibiotic Resistance Genes

Type

Blog Post

Published

Jan. 30, 2014, 3:29 p.m.

...Posts Learn How to Use Blue White Screening to Simplify Your Cloning Experiments See Where You Can Find...
Choosing a Good Mentor for Scientists

Type

Blog Post

Published

Jan. 16, 2014, 2:56 p.m.

...deserve your hard work and an advisor that will be a lifelong partner in developing your career in science. ...
History of CRISPR Cas - A tale of survival and evolution

Type

Blog Post

Published

Nov. 25, 2013, 10:12 p.m.

...a powerful and adaptive defense strategy against life-threatening viruses. Evolving to survive Although...
In Vivo Biotinylation of Bacterial Fusion Proteins

Type

Blog Post

Published

Jan. 25, 2018, 2:09 p.m.

...including surface plasmon resonance (Biacore, GE Life Sciences) or bio-layer interferometry (Octet, Pall...
Science Careers: Unruly Interests Feed Many Paths

Type

Blog Post

Published

Sept. 26, 2017, 12:12 p.m.

...walk one path at a time, the variety of paths in life is mind-boggling. Unlike a mountain – with many ...
5 Uncommon Ways to Gain Career Skills During an Uncommon Time

Type

Blog Post

Published

Oct. 20, 2020, 1:15 p.m.

... a few colleagues to work together on career and life goals. For tips on how to get started see Form Your...
Behind-the-scenes of the Isolation of the Thermostable IgnaviCas9 From a Yellowstone Hot Spring

Type

Blog Post

Published

Nov. 12, 2019, 2 p.m.

...dark matter – large tracts of the microbial tree of life that remained unexplored. Using new single-cell ...
Tetbow: Bright Multicolor Labeling for Neuronal Tracing

Type

Blog Post

Published

Jan. 24, 2019, 2:24 p.m.

...circuits with fluorescent proteins and chemical tags." eLife 7 (2018): e40350. PubMed PMID: 30454553. PubMed ...
Natural or synthetic? How Addgene’s dataset reveals trends in biological innovation

Type

Blog Post

Published

Nov. 8, 2018, 3:47 p.m.

...to source genes from farther away in the tree of life." Nature communications 9.1 (2018): 4425. PubMed...
Intro to Our Summer SciComm Series!

Type

Blog Post

Published

June 9, 2022, 1:15 p.m.

...sequence that uses trprimers and enzymes that will amplify a sequence only if present.” You can see how you...
Plasmids 101: Origin of Replication

Type

Blog Post

Published

Nov. 11, 2020, 2:23 p.m.

...sensitive; others ori's can be "tricked" into amplifying more copies with the addition of chloramphenicol...
Donations from Addgene to Yield Answers for Rare Disease Researchers

Type

Blog Post

Published

Sept. 15, 2015, 2:30 p.m.

...win some of the latest tools and technologies in life sciences research. The rare disease research community...
Advice for Moving Into Sales After Your Science Postdoc

Type

Blog Post

Published

Aug. 21, 2014, 3:15 p.m.

... like these are widely available, especially for life science researchers. Many biotechnology companies...
Interview: Ed Boyden on Optogenetics, Neuroscience, and the Future of Neuroengineering

Type

Blog Post

Published

March 4, 2014, 4:49 p.m.

... come from basically every branch on the tree of life. Our most recent paper in Nature Methods offers ...
Creating accessible biology activities in schools with BioBits

Type

Blog Post

Published

Aug. 2, 2018, 12:56 p.m.

...Arabidopsis research center brings plant science to life Resources on Addgene.org Browse our protocol videos...
"Build Your Plasmid": The Game - Play to Teach

Type

Blog Post

Published

July 17, 2018, 12:23 p.m.

...with us! This exchange of ideas will help improve life science and biomedical education for future generations...
FPbase: A new community-editable fluorescent protein database

Type

Blog Post

Published

May 16, 2018, 1 p.m.

...more powerful ways, and the advanced search page simplifies the process of building advanced multi-parametric...
Reagent Repositories Are Speeding up Science During the Pandemic

Type

Blog Post

Published

Aug. 4, 2020, 1:15 p.m.

...nonprofit repository for a variety of materials for life science research, including over 4,000 cell lines...
25 Work From Home Tips for Scientists

Type

Blog Post

Published

March 19, 2020, 1:15 p.m.

...stay virtually connected with the people in your life. At Addgene, we’ve started virtual activities like...
How Dry Ice Affects Viral Vector Sample pH & How to Avoid It

Type

Blog Post

Published

June 20, 2017, 2:30 p.m.

...Cleaver BD, Clément N, Byrne BJ, Zolotukhin S. A simplified purification protocol for recombinant adeno-associated...
With Our New Viral Service, We're Taking CRISPR Further!

Type

Blog Post

Published

Dec. 6, 2016, 3:30 p.m.

...gRNA library in plasmid format, a researcher must amplify the library, use next-generation sequencing to ...
Fluorescent Protein Travel Awards - Protein Variants, a Serotonin Sensor, and an Artificial Leaf Replica System

Type

Blog Post

Published

June 4, 2019, 1:02 p.m.

... incredibly important for nearly every aspect of life.” Unger began with the a bacterial periplasmic binding...
Visualizing Genomic Loci with CRISPR-Sirius

Type

Blog Post

Published

April 18, 2019, 12:53 p.m.

...al. “CRISPR-Sirius: RNA scaffolds for signal amplification in genome imaging.” Nature Methods 15 (2018)...
The Crispr Babies Saga Shows the Need for Action, Not More Delays

Type

Blog Post

Published

Dec. 20, 2018, 1:15 p.m.

...time?” She lives with her partner (in business and life) in an old Girl Scout Cookie factory with their ...
Addgene-Seeding Labs plasmid grant round 3: microbial drug resistance and biofilms, antidiabetic plant components, visualizing brain injury - and a glowing robot fish

Type

Blog Post

Published

Nov. 27, 2018, 2 p.m.

... 11 years he has studied various aspects extreme life on earth including extremophile microorganisms, ...
Unique and persistent IDs for improved reproducibility: Addgene now supports RRIDs and compact identifiers for all plasmids

Type

Blog Post

Published

Nov. 20, 2018, 8:17 p.m.

...PID formats. For example, several journals like eLife, Nature, and Cell use Research Resource identifiers...
Plasmids 101: Gram Negative and Positive Bacteria

Type

Blog Post

Published

Aug. 2, 2022, 1:15 p.m.

... M. Gram Staining. https://serc.carleton.edu/microbelife/research_methods/microscopy/gramstain.html Smith...
Celebrate National Book Month with Addgene

Type

Blog Post

Published

Oct. 3, 2019, 12:47 p.m.

...Anthologist. The book ended up literally changing my life. Though I have an undergraduate degree in English...
Four Factors that Differentiate the Stem Cell Field

Type

Blog Post

Published

Sept. 28, 2016, 2:35 p.m.

...cell field have described the Yamanaka paper as "life changing". Dr. Yamanaka himself, during his keynote...
R Bodies: Membrane-Rupturing Microscopic Tools

Type

Blog Post

Published

April 14, 2016, 2:30 p.m.

...conformational changes. These tiny movements are then amplified tens or hundreds of thousands of times by propagating...
Optimizing Donor DNA for Enhanced CRISPR Genome Editing

Type

Blog Post

Published

March 24, 2016, 2:30 p.m.

... by controlled timing of CRISPR/Cas9 delivery." Elife 3 (2015): e04766. PubMed PMID: 25497837. PubMed ...
Resume Writing for Non-academic Science Careers

Type

Blog Post

Published

Dec. 18, 2014, 4:06 p.m.

...years. Does an academic career work well with your life and career plan? This might be a tough question ...
Choosing Your Fluorescent Proteins for Multi-Color Imaging

Type

Blog Post

Published

Oct. 9, 2014, 3 p.m.

...fluorescent channels on the microscope. With the proliferation of fluorescent proteins in recent years, there...
Plasmids 101: Mammalian Vectors

Type

Blog Post

Published

March 25, 2014, 3:15 p.m.

...antigen (293E or 293T cells), allow for episomal amplification of plasmids containing the viral EBV or SV40...
Why and how to start studying chromatin (with Addgene's help)

Type

Blog Post

Published

June 12, 2018, 11:40 a.m.

...that those other components are still there in real life. Certainly referring to any DNA within the context...
Plasmids 101: Inducible Promoters

Type

Blog Post

Published

Jan. 18, 2018, 2:34 p.m.

...promoter and ceases repression of the target gene. A simplified lac inducible system is found in many bacterial...
A Novice's Perspective on Neuroscience - SfN2017

Type

Blog Post

Published

Dec. 15, 2017, 2:07 p.m.

...that will have impacts on many different facets of life. It also seems as if many of the experiments in ...
Quick Guide to Working with Drosophila Part 3: Genome Engineering in Flies

Type

Blog Post

Published

July 28, 2017, 1:30 p.m.

...can be dissected at various times throughout the lifespan of the fly to determine the implications of your...
Enabling Precision Functional Genomics with the Target Accelerator Plasmid Collection

Type

Blog Post

Published

May 11, 2017, 2:30 p.m.

...and allele function via Cell Painting. Rohban MH, Elife, 2017. PubMed PMID: 28315521. PubMed Central PMCID...
Viral Vectors 101: Types of viruses

Type

Blog Post

Published

June 6, 2023, 1:15 p.m.

...integration to express its cargo, in fact its viral life cycle does not include genomic integration. While...
Viral Vectors 101: The AAV Retrograde Serotype

Type

Blog Post

Published

April 27, 2023, 1:15 p.m.

...Janelia Research Campus and the University of California, Berkeley engineered an AAV variant that efficiently...
Plasmids 101: Screens vs. Selections

Type

Blog Post

Published

Nov. 10, 2022, 2:15 p.m.

... of enzymes: Library screening strategies. IUBMB Life, 61: 222-228 (2009). doi: https://doi.org/10.1002...
Early Career Researcher Toolbox: Social Media for Scientists

Type

Blog Post

Published

Dec. 11, 2018, 12:46 p.m.

...scientists can start using Twitter and provide real-life examples of how they and others have used Twitter...
Hot Plasmids December 2018 - C. elegans RNAi, TurboID, Detecting Viral Outbreaks, and Protein Binding Specificity Tools

Type

Blog Post

Published

Dec. 6, 2018, 1:34 p.m.

... to be easily concentrated. Viral RNA is then amplified isothermally, and detected through a toehold switch...
Viral Vectors 101: Gamma-Retroviral Packaging Systems

Type

Blog Post

Published

Aug. 6, 2024, 1:15 p.m.

...Addgene blog Viral Vector Elements The Retroviral Life Cycle Popular Retroviral Vectors and Their Use in...
Hot Plasmids - February 2022

Type

Blog Post

Published

Feb. 22, 2022, 2:15 p.m.

... the tag can recruit multiple frankenbodies to amplify the signal. Liu et al. Genes Cells. 2021. https...
A Quick Guide to a Career in Software Product Management

Type

Blog Post

Published

Nov. 24, 2020, 2:15 p.m.

...explore a career in product management. A day in the life Now that you know a bit about what a software product...
CRISPR 101: Non-Homologous End Joining

Type

Blog Post

Published

April 16, 2015, 3:45 p.m.

...together, thus repairing the break. This is a simplified, streamlined version of this pathway and does...

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