We narrowed to 317 results for: pho

1. Summer SciComm: Preprints

   Type

   Blog Post

   Published

   Aug. 16, 2022, 1:15 p.m.

   ... CERN — it's a new age of accelerating science! Photo courtesy of Henry Mühlpfordt, under a CC BY-SA license...

2. Summer SciComm Series: Cognitive Load

   Type

   Blog Post

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   June 16, 2022, 1:15 p.m.

   ... the slide deck (or reminding them to put their phones away), or maybe I was assuming something was intuitive...

3. Tagging Optogenetics and Chemogenetics Receptors: Fluorescent Proteins and Other Options

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   Blog Post

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   May 11, 2021, 1:15 p.m.

   ...expression of transgenes of interest. Furthermore, fluorophore variants (for example green and red fluorescent...

4. Transferable Skills Guide: Public Speaking

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   Blog Post

   Published

   April 20, 2023, 4 p.m.

   ...Public speaking is not just one of the most common phobias–it’s a key part of being a scientist and an incredibly...

5. Enhancing CRISPR Targeting Specificity with eSpCas9, SpCas9-HF1, & HypaCas9

   Type

   Blog Post

   Published

   Dec. 16, 2015, 3:30 p.m.

   ...al disrupted interactions between Cas9 and the phophate backbone of the target DNA strand via mutations...

6. Teaching an Old DOG New Tricks: Controlling Protein Activity with GFP

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   Blog Post

   Published

   Nov. 24, 2015, 3:30 p.m.

   ...FPs optimized for oxidizing environments to photoconvertible variants, it seems like GFP is always learning...

7. Writing Scientific Manuscripts: Literature Searching, Reading, & Organizing

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   Blog Post

   Published

   May 5, 2015, 3:54 p.m.

   ...Louisiana State University. She is a fan of photosynthesis and writes about autotrophs and biochemistry...

8. CRISPR 101: Anti-CRISPR Proteins Switch Off CRISPR-Cas Systems

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   Blog Post

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   July 23, 2020, 1:20 p.m.

   ...CRISPR-Cas activity. For example, fusing AcrA4II to the photo-sensitive LOV2 domain allows anti-CRISPR activity...

9. How to Prepare for an Industry Interview

   Type

   Blog Post

   Published

   July 9, 2020, 1:15 p.m.

   ...PI of the lab and for one position, I just had a phone interview. I also transitioned between different...

10. Nanoblades: Tiny CRISPR Ninjas for Genome Editing Difficult Cells

    Type

    Blog Post

    Published

    Sept. 26, 2019, 12:50 p.m.

    ...change to large insertions like the addition of a fluorophore or tag. To utilize HDR, a donor template containing...

11. Overcoming the Challenges of Lentiviral Production

    Type

    Blog Post

    Published

    Aug. 7, 2018, 12:31 p.m.

    ...transfection method (i.e. polyethyleneimine, calcium phosphate or liposomal methods), the volume of the reaction...

12. Popular Retroviral Vectors and Their Uses in Scientific Research

    Type

    Blog Post

    Published

    July 24, 2018, 12:51 p.m.

    ...genes via infection with a single ecotropic or amphotropic MuLE lentivirus provides significant genetic ...

13. Adenoviral Vector Production and Troubleshooting

    Type

    Blog Post

    Published

    June 14, 2018, 11:44 a.m.

    ...looking at absorbance at 260nm using UV/Vis spectrophotometry. An absorbance of at 260nm corresponds to...

14. Plasmids 101: In-house NGS Plasmid Quality Control Now at Addgene

    Type

    Blog Post

    Published

    April 6, 2021, 12:30 p.m.

    ...quantification of various intermediate steps, a sizing electrophoretic gel, and qPCR quantification of the final libraries...

15. Hot Plasmids and Viral Preps - March 2021

    Type

    Blog Post

    Published

    March 23, 2021, 1:15 p.m.

    ....   G-baToN can also be applied to other fluorophore/antibody pairs. Because the anti-GFP nanobody...

16. Quickest Way to Deposit Plasmids: The Deposit Spreadsheet

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    Blog Post

    Published

    March 8, 2017, 3:15 p.m.

    ... Addgene account username. Shipping address and phone number. Plasmid sequences or GenBank files. We encourage...

17. CRISPR in the Clinic

    Type

    Blog Post

    Published

    Nov. 2, 2023, 1:15 p.m.

    ...CRISPR spin. CRISPR CAR-T treatments for leukemia, lymphoma, and some solid tumors are currently in early-...

18. Plasmids 101: Modular Cloning

    Type

    Blog Post

    Published

    March 12, 2024, 1:15 p.m.

    ...Level 1 TUs with unique promoters, and the ideal phosphatase (or any modifying enzyme) to pair with it can...

19. Hot Plasmids February 2024

    Type

    Blog Post

    Published

    Feb. 20, 2024, 2:45 p.m.

    ...Ando et al., 2023). This variant has similar photostability and brightness compared to the original, though...

20. 5 Tips for Troubleshooting Viral Transductions

    Type

    Blog Post

    Published

    Aug. 11, 2016, 2:23 p.m.

    ...fibronectin-enhanced retroviral transduction of primary human T lymphocytes for adoptive immunotherapy." Acute Leukemias ...

21. Gaining Leadership Skills Volunteering at a Professional Organization

    Type

    Blog Post

    Published

    Aug. 6, 2015, 2:30 p.m.

    ... with other people mostly online and on monthly phone calls. This has its challenges as meeting in person...

22. 7 Simple Strategies to Resolve Conflicts with Difficult Supervisors

    Type

    Blog Post

    Published

    Jan. 21, 2015, 1:30 p.m.

    ...discuss important issues in person or over the telephone instead of email. As you cannot see a person’s...

23. Mobile-CRISPRi: Bringing CRISPRi to Diverse Bacteria

    Type

    Blog Post

    Published

    April 4, 2019, 12:53 p.m.

    ... of the highly conserved glutamine-fructose-6-phosphate aminotransferase (glmS) gene in the recipient ...

24. Degrading DNA with Cascade-Cas3

    Type

    Blog Post

    Published

    Feb. 11, 2025, 2:15 p.m.

    ...Yoshimi, K., Okuzaki, Y., Gee, P., Kunihiro, Y., Sonpho, E., Xu, H., Sasakawa, N., Naito, Y., Nakada, S...

25. Hot Plasmids and Viral Preps - May 2021

    Type

    Blog Post

    Published

    May 18, 2021, 1:15 p.m.

    ...page.  A new system uses light to deactivate photocleavable gRNAs within seconds. A CRISPR-based lineage...

26. AAV Vector Quality Control: Going the Extra Mile with NGS

    Type

    Blog Post

    Published

    Sept. 12, 2017, 1:44 p.m.

    ...’d like us to discuss. We are also available by phone or at [email protected] to answer your questions ...

27. Cloning Mammalian Cells with the Agarose Method

    Type

    Blog Post

    Published

    Sept. 7, 2017, 12:17 p.m.

    ...using cloning rings in terms of growth rates, morphology or other physiological properties. An advantage...

28. Quick Guide to Working with Drosophila Part 1: Getting Started with Flies

    Type

    Blog Post

    Published

    July 13, 2017, 2:30 p.m.

    ...mother. Inside the pupal case, the larva will metamorphose into its adult form. In all, it takes roughly...

29. Fluorescent Proteins 101: Visualizing Subcellular Structures & Organelles

    Type

    Blog Post

    Published

    June 22, 2017, 2:30 p.m.

    ...setup for a different fluorescent signal) and photobleaching i.e. FP stability. One should also be aware...

30. Fluorescent Proteins 101: Fluorescent Protein Timers

    Type

    Blog Post

    Published

    May 4, 2017, 2:30 p.m.

    ...change is usually hypothesized to be a result of chromophore oxidation. Some of the more popular timers (whether...

31. Starter guide to induced pluripotent stem cells (iPSCs) part 1:  A renaissance in regenerative medicine

    Type

    Blog Post

    Published

    Oct. 30, 2018, 1:04 p.m.

    ...form teratomas [11]. iPSCs also possess similar morphologies, growth properties, and express stem cell markers...

32. Fluorescent Tagging of Endogenous Genes with SapTrap

    Type

    Blog Post

    Published

    Oct. 12, 2017, 2:26 p.m.

    ...about Fluorescent Protein Timers Check out Photoactivatable Fluorescent Proteins Use Rosella to Monitor...

33. Genome-wide Screening Using CRISPR

    Type

    Blog Post

    Published

    Aug. 18, 2015, 2:30 p.m.

    ...a given phenotype. Image Credit: www.flickr.com/photos/72005145@N00/5600978712/ What CRISPR-based ...

34. Don’t FRET: Bimolecular Fluorescence Complementation Makes Visualizing Protein-Protein Interactions Easy

    Type

    Blog Post

    Published

    Aug. 27, 2020, 1:15 p.m.

    ...non-radiative transfer for energy between neighboring fluorophores, FRET can be used to detect the inter- and intramolecular...

35. Great Results Start with Great Standard Curves

    Type

    Blog Post

    Published

    Aug. 15, 2024, 1:15 p.m.

    ... absorbance of both is then measured on a spectrophotometer and concentration versus absorbance is plotted...

36. CRISPR 101: Ribonucleoprotein (RNP) Delivery

    Type

    Blog Post

    Published

    Sept. 6, 2018, 12:02 p.m.

    ...Cas9, such as visualizing genomic loci using fluorophore tagged dCas9 may require the use of plasmid or...

37. Simplify Cloning with in vivo Assembly

    Type

    Blog Post

    Published

    Oct. 18, 2018, 12:37 p.m.

    ...unamplifiable’ Check your amplification by gel electrophoresis. In our experience, good amplification after...

38. Plasmids 101: Protein tags

    Type

    Blog Post

    Published

    Dec. 11, 2014, 4:26 p.m.

    ...tag for purification? Or you want to combine a fluorophore with a tag that localizes your protein to the...

39. Binning Singletons: Tackling Conference Networking When You Don’t Know Anyone

    Type

    Blog Post

    Published

    Feb. 18, 2020, 2:15 p.m.

    ...provided moral support was invaluable.  Mixing metaphors and scientists - Culturing a scientific network...

40. Save Time with Transient Plant Leaf Transformations

    Type

    Blog Post

    Published

    July 25, 2019, 12:03 p.m.

    ...used in C. roseus, but processes specific to photosynthetic active tissues cannot be studied in hairy roots...

41. Designing Your Chalk Talk for the Academic Job Interview

    Type

    Blog Post

    Published

    March 12, 2019, 1:37 p.m.

    ...distance runner, gardener, cook, and outdoor photographer.  Additional resources on the Addgene blog ...

42. Antibodies 101: The Four ELISAs and When to Use Them

    Type

    Blog Post

    Published

    July 25, 2024, 1:15 p.m.

    ... as horseradish peroxidase (HRP) or alkaline phosphatase (AP). Fluorescent tags may also be used, but ...

43. Hot Plasmids Spring 2024

    Type

    Blog Post

    Published

    May 23, 2024, 1:15 p.m.

    ...6).   Figure 6: Illustration of split fluorophore assay to test reconstitution via mRNA trans-splicing...

44. CRISPR 101: Targeting RNA with Cas13a (C2c2)

    Type

    Blog Post

    Published

    Sept. 21, 2017, 2:07 p.m.

    ...will be cleaved resulting in activation of the fluorophore. The fluorescent signal can be used as an indicator...

45. Hot Plasmids - November 2023

    Type

    Blog Post

    Published

    Nov. 14, 2023, 3:35 a.m.

    ... promoter. For example, neurons treated with a phorbol ester activator gave fluorescent signal in the ...

46. Plasmids 101: Broad Host Range Plasmids

    Type

    Blog Post

    Published

    April 11, 2024, 1:15 p.m.

    ...Gluconacetobacter xylinus, Gluconobacter spp., Hyphomicrobium spp., Klebsiella aerogenes, K. pneumoniae, ...

47. 5 Reasons to Use Reddit for Science Communication

    Type

    Blog Post

    Published

    Oct. 20, 2016, 2:30 p.m.

    ...notes which Evernote even allows me to access on my phone. I use it for everything from receipts to blogs ...

48. Healthcare Consulting: A Door to the Business of Life Sciences

    Type

    Blog Post

    Published

    Oct. 13, 2016, 2:30 p.m.

    ...chemotherapies, or developing innovative mobile phone applications and services that help diabetic patients...

49. Bacterial Toxin-antitoxin Systems as Molecular Biology Tools

    Type

    Blog Post

    Published

    Nov. 1, 2018, 12:35 p.m.

    ... regulated by guanosine [corrected] 3', 5'-bispyrophosphate: a model for programmed bacterial cell death...

50. Transferable Skills Guide: Career Planning Resources

    Type

    Blog Post

    Published

    Nov. 14, 2017, 2 p.m.

    ...HR, greatly increasing your chances of getting a phone interview! If you don’t have a particular employer...