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  1. 5 Great Apps for Lab Life

    Type
    Blog Post
    Published
    Sept. 22, 2016, 2:30 p.m.
    ...paper lab notebooks you’re saving trees! The Earth thanks you. 2. Gibco Cell Culture by Gibco Whenever I ...please share it in the comments section below! Many thanks to our guest blogger Sean Stacey! Sean D. Stacey...

  2. Using CRISPR/Cas9 to Edit Disease Out of the Genome

    Type
    Blog Post
    Published
    June 23, 2022, 1:15 p.m.
    ... in organoid cultures are stable,” said Gerald Schwank, first author of the cystic fibrosis report. “This...Cell. 13, 659-662 (5 December 2013). 8. Gerald Schwank et al. "Functional Repair of CFTR by CRISPR/Cas9...

  5. Behind the scenes: Addgene’s new search engine and more

    Type
    Blog Post
    Published
    Dec. 12, 2019, 2:22 p.m.
    ...multidisciplinary effort and cross-team collaboration (thanks to all Addgenies involved). The new homepage and...example, matches of a name in the depositor field will rank higher than matches of the same name in the publication...

  6. 15 Years of Addgene: The Top 15 Plasmids

    Type
    Blog Post
    Published
    Jan. 8, 2019, 1:54 p.m.
    ...Find pSpCas9(BB)-2A-Puro (PX459). Thank you! We would like to thank all of our depositors and requestors...

  7. Antibodies 101: Buffers, Storage, and Conjugates

    Type
    Blog Post
    Published
    Feb. 1, 2022, 2:15 p.m.
    ...buffer, which will be noted on the product sheet. Thankfully, sodium azide can easily be removed from the ...complex subjects in antibody-based protocols - but thankfully this is an Antibody 101 post, so we'll keep it...

  8. Dos & Don'ts When Publishing a Scientific Manuscript

    Type
    Blog Post
    Published
    May 22, 2014, 4:02 p.m.
    ...authors are asked to add an identifier (a bit like a GenBank ID) to all antibodies, animal models, and software...publishing your manuscript, or add your own tips below. Thank You to Our Guest Blogger! Maaike Pols is a Developmental...

  9. Transferable Skills Guide: Public Speaking

    Type
    Blog Post
    Published
    April 20, 2023, 4 p.m.
    ... lost in a sea of data, my mind went completely blank. After several seconds of truly panicked silence... so they could understand it. When my mind went blank, my options were to stop talking until I could present...

  10. To Each HIS Own

    Type
    Blog Post
    Published
    March 2, 2023, 2:15 p.m.
    ...and can elute proteins with up to 95% purity (Janknecht 1991, Hochuli 1988). In this purification approach...Adsorbent. Bio/Technology. 1988; 6: 1321–1325. Janknecht R, de Martynoff G, Lou J, Hipskind RA, Nordheim...

  11. CRISPR 101: Cas9 vs. The Other Cas(s)

    Type
    Blog Post
    Published
    Dec. 8, 2022, 2:15 p.m.
    ...editing. Fast facts – PAM requirement: protospacer flanking sequence – A, U, or C. Best for: targeting single...use. Fast facts – PAM requirement: protospacer flanking sequence – none. Best for: targeting single stranded...

  12. Summer SciComm Series: Cognitive Load

    Type
    Blog Post
    Published
    June 16, 2022, 1:15 p.m.
    ... provide detailed evidence of knowledge new to mankind. And you probably understand how much faster it...communicate. It’s okay (and encouraged) to leave blank spaces or to gloss over some aspects when needed...

  13. New Tool for Lineage Tracing: The ClonTracer Library

    Type
    Blog Post
    Published
    Sept. 22, 2015, 2:30 p.m.
    ...library was developed when Carrie was a post-doc in Frank Stegmeier’s lab in Novartis Oncology.  Bhang did...ways you put the ClonTracer library to use! Many thanks to Carrie and Novartis for taking the time to talk...

  14. CRISPR in the Clinic

    Type
    Blog Post
    Published
    Nov. 2, 2023, 1:15 p.m.
    ...isolated from two patients with cystic fibrosis (Schwank et al). They were able to demonstrate functional...Cas9." Cell Stem Cell. 13, 659-662 (2013). Gerald Schwank et al. "Functional Repair of CFTR by CRISPR/Cas9...

  15. A Guide to Designing a Scientific Poster: Content, Layout, and More

    Type
    Blog Post
    Published
    Oct. 22, 2019, 12:20 p.m.
    ...majority of the visual data. This central box is then flanked by two equally-sized columns, where the introduction...sections like the introduction, methods, and figures flank the central region, and a scannable barcode is included...

  16. Design Tips for Prime Editing

    Type
    Blog Post
    Published
    Jan. 23, 2025, 2:15 p.m.
    .... J., Hussmann, J. A., Yan, J., Knipping, F., Ravisankar, P., Chen, P.-F., Chen, C., Nelson, J. W., Newby...j.cell.2014.02.001 Yan, J., Oyler-Castrillo, P., Ravisankar, P., Ward, C. C., Levesque, S., Jing, Y., Simpson...

  17. Plasmids for Endogenous Gene Tagging in Human Cells

    Type
    Blog Post
    Published
    April 6, 2017, 1:02 p.m.
    ...whole segment—a GFP tag and 1kb of homologous DNA flanking both sides (about 2.7kb total)—is inserted into... use genomic tagging in your experiments. Many thanks to our guest bloggers from the Allen Institute ...

  19. Mouse Modeling, Part 1: Genetically Engineered Mice

    Type
    Blog Post
    Published
    July 11, 2019, 1:26 p.m.
    ...site within the genome using homology arms that flank the gene of interest. In either case, a positive... sites are inserted directly into the genome to flank the gene that will be deleted following Cre-mediated...

  20. The Materials Science of Optogenetics Experiments

    Type
    Blog Post
    Published
    Sept. 17, 2015, 2:30 p.m.
    ...deliver light pulses to the target brain region. Thankfully, due to their prominence, the preparation of ...performing optogenetics behavioral experiments. Thank you to our guest blogger Derek Simon! Derek P....
Showing: 101 - 120 of 440 results