We narrowed to 149 results for: CO

1. Cloning Mammalian Cells with the Agarose Method

   Type

   Blog Post

   Published

   Sept. 7, 2017, 12:17 p.m.

   ... under-expressing clones. If GFP-encoding cDNA is co-transfected at a favorable ratio to the gene of interest...

2. Google Forums Round Up: First Impressions of NgAgo

   Type

   Blog Post

   Published

   Aug. 4, 2016, 2:30 p.m.

   ... with an ssDNA guide. Therefore, researchers must co-transfect cells with 5’-P-ssDNA guides and an NgAgo...

3. Fluorescent Proteins 101: Green Fluorescent Protein (GFP)

   Type

   Blog Post

   Published

   May 15, 2014, 3:33 p.m.

   ...chromophore forms spontaneously and without additional co-factors, substrates, or enzymatic activity – it only...

4. Your Lentiviral Plasmid FAQs Answered

   Type

   Blog Post

   Published

   April 23, 2014, 1:08 p.m.

   ...explains how lentiviral particles are produced by co-transfecting three essential ingredients: the lentiviral...

5. Drew Endy Introduces the Biobrick Public Agreement Plasmid Collection

   Type

   Blog Post

   Published

   Dec. 12, 2013, 3:35 p.m.

   ...data and computing inside living cells. He's also a co-founder of the BioBricks Foundation, an organization...

6. INTEGRATE: Bacterial Genome Engineering Using CRISPR-Transposons

   Type

   Blog Post

   Published

   Dec. 7, 2020, 2:53 p.m.

   ... own nuclease activity. Instead, it forms a novel co-complex with the transposition protein TniQ for targeting...

7. Don’t FRET: Bimolecular Fluorescence Complementation Makes Visualizing Protein-Protein Interactions Easy

   Type

   Blog Post

   Published

   Aug. 27, 2020, 1:15 p.m.

   ... BiFC constructs. If your PIPs actually interact, co-expressing one of the endogenous PIPs should decrease...

8. Antibodies 101: Immunoprecipitation

   Type

   Blog Post

   Published

   Dec. 7, 2021, 2:15 p.m.

   ...linkage. This means that the primary antibody will not co-elute with the target protein, eliminating the risk...

9. AAVs in Retinal Gene Therapy

   Type

   Blog Post

   Published

   July 31, 2018, 12:55 p.m.

   ...a result, Luk Vandenberghe and colleagues at MEEI co-founded a non-profit to help push forward candidate...

10. New Tools Enable CRISPRa for Neuroscience Applications

    Type

    Blog Post

    Published

    July 2, 2019, 12:51 p.m.

    ...out two Bdnf isoforms (I and IV) for investigation. Co-transduction of primary rat neurons with dCas9-VPR...

11. Course-Based Undergraduate Research Experiences (CUREs)

    Type

    Blog Post

    Published

    Feb. 9, 2023, 2:15 p.m.

    ...barriers to implementation. Funded by the NSF (with Co-PIs Jessica Bell, Lisa Gentile, Ellis Bell, and Amy...

12. Tips for a 1st time AAV user (by a Rookie AAV user)

    Type

    Blog Post

    Published

    Oct. 23, 2018, 11:49 a.m.

    ...intimidating, but I had my lab’s protocols and some great co-workers to help me out. Even with these tools, I ...

13. Simplify Cloning with in vivo Assembly

    Type

    Blog Post

    Published

    Oct. 18, 2018, 12:37 p.m.

    ...fragment, encode homology arms to allow immediate co-transformation with linearized vector backbone. ...

14. AAVs CREATed for Gene Delivery to the CNS and PNS

    Type

    Blog Post

    Published

    Sept. 28, 2017, 2:01 p.m.

    ...expressing the inducer (i.e. tet-off transactivator) is co-injected. The dose of this AAV can be adjusted to...

15. Lentiviral Vector Uses and Overview

    Type

    Blog Post

    Published

    May 19, 2016, 2:30 p.m.

    ...viral packaging and host genome integration. After co-transfection of these plasmids, 293T cells released...

16. Minigenomes - a Safe Way to Study Dangerous Viruses Like the Ebola Virus

    Type

    Blog Post

    Published

    March 17, 2016, 2:30 p.m.

    ...genes are each encoded on separate plasmids and are co-transfected into cells alongside a plasmid encoding...

17. Avoiding the Dark Side of Fluorescent Protein Fusions with mOX FPs

    Type

    Blog Post

    Published

    Oct. 27, 2015, 3 p.m.

    ...University of North Carolina at Chapel Hill. Under the co-mentorship Blossom Damania and Jack Griffith, her...

18. Negotiating Work and Life: How to Find the Joy

    Type

    Blog Post

    Published

    March 20, 2014, 3:04 p.m.

    ...work and make this happen. Be realistic and: Your co-workers will learn to work within these bounds You...

19. Overcoming the AAV Size Limitation for CRISPR Delivery

    Type

    Blog Post

    Published

    Sept. 16, 2020, 1 p.m.

    ...packaging capacity but necessitating purification and co-infection of two AAVs. Cas9 orthologs: shorter, ...

20. Binning Singletons: Tackling Conference Networking When You Don’t Know Anyone

    Type

    Blog Post

    Published

    Feb. 18, 2020, 2:15 p.m.

    ... General Meeting (now Microbe) in 2013 in Denver, CO. While I had been to the General Meeting seven other...