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Addgene

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We narrowed to 148 results for: Coli

Showing: 101 - 120 of 148 results

  2. Antibodies 101: Affinity Reagents

    Type
    Blog Post
    Published
    April 14, 2022, 1:15 p.m.
    ...specificity and binding affinity, can be produced in E. coli, and are both soluble and easily engineered.  Monobodies...

  4. CRISPR in the Clinic

    Type
    Blog Post
    Published
    Nov. 2, 2023, 1:15 p.m.
    ...infections. The Cas3 enzymes target several strains of E. coli and lethally chop their genomes into many, many ...

  5. Genome-wide Screening Using CRISPR

    Type
    Blog Post
    Published
    Aug. 18, 2015, 2:30 p.m.
    ...CRISPR libraries that target mouse, human, fly, E. coli, and T. gondii genes.  2) What genetic modification...

  8. Pooled Library Amplifications

    Type
    Blog Post
    Published
    May 30, 2024, 1:30 p.m.
    ...transformation amplification protocol is the Chong Zhang E. coli Genome-wide Inhibition Library. DNA and cell amounts...

  10. Anatomy of a Plasmid Page at Addgene

    Type
    Blog Post
    Published
    Feb. 4, 2016, 3:30 p.m.
    ...  Most Addgene plasmids are maintained in the E. coli lab strain DH5a, but others may require strains ...

  11. Hot Plasmids - August 2020

    Type
    Blog Post
    Published
    Aug. 18, 2020, 1:15 p.m.
    ...Nanobodies are easily expressed in the cytosol of E. coli, however the nanobody proteins tend to get stuck...inclusion body problem and makes nanobody production in E.coli as simple as following a DNA miniprep protocol!... were expressed in SHuffle T7 Express competent E.coli (NEB) using IPTG induction. To biotinylate the ...

  13. Neuronal labeling with Spaghetti Monster

    Type
    Blog Post
    Published
    Aug. 14, 2018, 12:42 p.m.
    ...strep-tag and, more recently, OLLAS (Escherichia coli OmpF linker and mouse langerin) and Sun Tag (Viswanathan...

  14. Plasmids 101: Secondary Nanobody Toolbox

    Type
    Blog Post
    Published
    Feb. 27, 2018, 2:04 p.m.
    ...Activity Learn more about protein tags Read about E. coli strains for protein expression Additional Resources...

  15. Fluorescent Proteins 101: Aptamer Fluorophores

    Type
    Blog Post
    Published
    April 11, 2017, 2:30 p.m.
    ...fluorescence-activated cell sorting (FACS) of E. coli transformed with aptamer libraries, the Jaffrey ...nucleotide aptamer they called Broccoli. As a product of SELEX and FACS, Broccoli is inherently more stable...new RNA-based scaffolds to contain and stabilize Broccoli cassettes, thereby extending their use into mammalian...mammalian cells. They’ve also dimerized Broccoli to further enhance its biosensor capabilities (see Fig...et al. “In-gel imaging of RNA processing using broccoli reveals optimal aptamer expression strategies....Addgene.org Find the Spinach Plasmids at Addgene Find Broccoli Plasmids at Addgene View All Fluorescent Protein...

  16. Viral Production at Addgene

    Type
    Blog Post
    Published
    Oct. 24, 2023, 1:15 p.m.
    ...propagated in the endA-mutated NEB Stable strain of E. coli. In addition, plasmids are typically prepared using...

  18. CRISPR Antimicrobials

    Type
    Blog Post
    Published
    May 3, 2016, 2:30 p.m.
    ...beta-lactam and quinolone resistance genes in E. coli using plasmid and phagemid delivery systems. Phagemids...
Showing: 101 - 120 of 148 results