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Showing: 101 - 120 of 371 results

  1. Developing a cold tolerant E. coli using specialized chaperones

    Type
    Blog Post
    Published
    Jan. 28, 2020, 2:35 p.m.
    ... biosensor - IISER Bhopal is back in the SynBio arena this year with a fresh team of twenty excited undergrads...expression under low temperature conditions, thereby enabling efficient CRISPR-Cas mediated editing for psychrophilic...

  2. Viral Vectors 101: Biosensors

    Type
    Blog Post
    Published
    July 6, 2023, 1 p.m.
    ...simply – they are systems that measure biological phenomena by generating an output which is directly proportionate...directly measure a cellular process. Cellular phenomena such as exocytosis, autophagy, voltage, and tension...

  3. Viral Vectors 101: The AAV Retrograde Serotype

    Type
    Blog Post
    Published
    April 27, 2023, 1:15 p.m.
    ...the AAV retrograde serotype (AAVrg) and how it enables scientists to access neurons, and study their connections... across broader physical areas of the brain and enable access to neurons that are physically blocked, ...

  5. dTAG - You're it!

    Type
    Blog Post
    Published
    June 21, 2018, 2:06 p.m.
    ...Bradner and Gray labs, we synthesize compounds that enable selective removal of proteins-of-interest from ...that are available from Addgene. These plasmids enable lentiviral expression of N- or C-terminally FKBP12F36V-tagged...

  9. Pairing CombiGEM and CRISPR for Combinatorial Genetic Screening

    Type
    Blog Post
    Published
    April 12, 2016, 2:30 p.m.
    ...Enhanced killing of antibiotic-resistant bacteria enabled by massively parallel combinatorial genetics." ...al. "Multiplexed barcoded CRISPR-Cas9 screening enabled by CombiGEM." Proceedings of the National Academy...

  10. Addgene's Tips for Plasmid Quality Control

    Type
    Blog Post
    Published
    Jan. 14, 2016, 3:30 p.m.
    ...Other information may also be critical to the maintenance and use of certain plasmids, such as special ...for the inserts or the full plasmids. This will enable you to quickly BLAST your sequencing result against...

  11. Plasmids for Endogenous Gene Tagging in Human Cells

    Type
    Blog Post
    Published
    April 6, 2017, 1:02 p.m.
    ...transfect the cells, these large regions of homology enable us to effectively utilize the host cell’s inherent...tagging with fluorescent proteins as described here enables live cell imaging to study cellular structures ...

  12. Popular Retroviral Vectors and Their Uses in Scientific Research

    Type
    Blog Post
    Published
    July 24, 2018, 12:51 p.m.
    ...integrate permanently into the host genome and thereby enable long-term stable gene expression. Gamma-retroviral.... This empty MuLV vector from the Weinberg lab enables researchers to express siRNAs of their choice in...

  14. Rabies and Neuronal Tracing

    Type
    Blog Post
    Published
    May 29, 2018, 1:51 p.m.
    ...that blood flows through the heart and lungs and oxygenates the body. However, in neuroscience, this architecture... (e.g., optogenetic or chemogenetic receptors) enables complex control of specifically defined neuronal...

  16. Nanoblades: Tiny CRISPR Ninjas for Genome Editing Difficult Cells

    Type
    Blog Post
    Published
    Sept. 26, 2019, 12:50 p.m.
    ...after Nanoblade treatment as measured by their maintenance of pluripotency marker expression and response...Nanoblades targeting the hydroxyphenylpyruvate dioxygenase (HPD) gene, all nine treated mice had ~10% editing...

  17. Corralling and Cornering the Goldilocks Antibody

    Type
    Blog Post
    Published
    Aug. 9, 2022, 1:15 p.m.
    ...Allen Brain Atlas. The comparison with two atlases enables us to see the reproducibility and accuracy while...from antibodies that we screen. This atlas will enable scientists to have an intuitive guide for checking...

  20. Plasmids 101: The Wide World of Natural Plasmids

    Type
    Blog Post
    Published
    Oct. 8, 2024, 1:15 p.m.
    ...toxic substances like heavy metals or pesticides, enabling their hosts to survive where others can’t. This...diversity of natural plasmids and their functions has enabled researchers to develop and deposit the many plasmid...
Showing: 101 - 120 of 371 results