We narrowed to 559 results for: ESP

...to a large family of solute channels that are responsible for maintaining the nutrient and ionic balance...of an autoinflammatory loop: keratinocytes both respond to and release IL-36 cytokines. Keratinocytes are...cell and translate those signals into cellular responses. For example, GPCRs and their downstream G protein...

...scientific publishing has a number of drawbacks, especially the increasingly growing time from submission... a few individuals.  Early-career researchers especially enjoy the ability to post a preprint as evidence...editors’ comments, reviewers’ comments, and author responses can be shared directly alongside the preprint ...

...stimulates the overall immune response. Once the initial antibody response starts to wane about a month...include any antibodies produced during the immune response.   Polyclonal antibodies are generated ...

...everything. If it’s barcoded, it should have a corresponding entry in your database. Enjoy your happy lab...at some point, decide early on what to do in a namespace collision. It may even be worth it to have extra... you're solving, but consistency is key. Some namespace collisions you may encounter: vector, sequence...

...cyclization recombinase”), is a 38 kDa protein responsible for intra- and inter-molecular recombination ...lox system is a well-established research tool, especially in the field of mouse transgenics. A few of the... (FRT) site being analogous to Cre and loxP, respectively. The FLP-FRT technology can be an effective ...

...lack all viral genes, including those that are responsible for integration into host chromosomes, further... (HDR). This characteristic makes AAV vectors especially amenable to correcting disease-associated mutations...PMCID: PMC4450362. 4. Sun, J. Y., et al. "Immune responses to adeno-associated virus and its recombinant ...

... using AdV is that it triggers a strong immune response after in vivo administration, which results in...expression ceases relatively quickly due to immune response against AdV. Importantly, recombination-competent...spectrophotometry. An absorbance of at 260nm corresponds to 1.1 x 1012 particles/mL. To calculate the ...

... and AsCpf1 are 3.7 kb and 3.9 kb in length, respectively, larger than SaCas9 but smaller than SpCas9....These nucleases should fit well into AAV vectors, especially given that Cpf1 gRNAs are also shorter than those...rate is similar to that of high fidelity Cas9s eSpCas9 and SpCas9-HF1. Both AsCpf1 and LbCpf1 ribonucleoproteins...

...communication and learning to work with people despite the distance improved my verbal and written communication...strengths and work effectively. Leadership responsibilities Being a leader in an organization, you need... different hats and came to understand the responsibilities that come with leadership. The NPA was a great...

... the spotlight. TnpBs are gaining popularity, especially among plant biologists, due to their compact ...Pre-existing stem cell heterogeneity dictates clonal responses to the acquisition of leukemic driver mutations... ex vivo cell engineering applications and is especially well-suited for lung cells via airway administration...

...there’s a PAM near your insertion site, the corresponding gRNA might be undesirable due to potential off-target...than SpCas9, in this case, NNGRRT and NNNNGATT, respectively. Researchers also engineered SpCas9 variants..., resulting in lower overall genome frequency. Despite the progress we’ve made, there is still a need ...

...with the Krauthammer lab (University of Zurich), especially with my deep learning-wizard colleague Ahmed ...selecting the most promising pegRNA designs and especially facilitates the use of prime editing in pooled...true for any prime editor experimentalist, and especially shines once large-scale prime editing experiments...

...expensive to gain access to routinely, and it can be especially challenging when trying to develop antibodies... a defined set of different trinucleotides (corresponding to different amino acids) in the regions that...further characterization and analysis. In a rapid response to the COVID-19 pandemic, the Seeger lab demonstrated...

... on which lab jobs needed to be done (Who is responsible for making the running buffer this week?). Scientists...what is most important to each person. It can especially be difficult to think creatively when you are...create a win-win situation? Learning about and respecting cultural differences is a crucial part of understanding...

...fluorescent proteins, it’s important to note that despite their distinction by name, chromoproteins are actually...pTOX6 express the YhaV, MqsR, and Tse2 toxins, respectively, and all encode the amilCP chromoprotein. Vectors...pTOX9 express the YhaV, MqsR, and Tse2 toxins, respectively, and encode the tsPurple chromoprotein.  Find...

...are also an important target for gene delivery, especially for the study of pain. While many new capsids...and AAV.PHP.S, that target the CNS or the PNS, respectively.  Read on to learn more about these two new ...Cre-dependent selection yields AAV variants for widespread gene transfer to the adult brain. Nature biotechnology...

...without triggering a destructive inflammatory immune response. At the time, adenoviral vectors were the most...disease-causing genes, including the RPE65 gene responsible for an autosomal recessive, severe, childhood-onset...follow in the footsteps of Luxturna™. However, despite promising results in preclinical studies some potential...

...individual molecules. Poor photon budgets are a widespread issue with fluorescent proteins—even the thriftiest...student searching couch cushions for spare change, desperate to extract a few more photons from a sample. Chemical...have excellent properties inside living cells, especially for advanced imaging experiments. We continue...

...and it would have been very difficult for us to respond to such a large number of requests by ourselves...with each other, which is critically important, especially for young researchers. It was a really nice incubator...Identification of Proteins Associated with an IFNgamma-Responsive Promoter by a Retroviral Expression System for...

...facilitate binding and cleavage of target DNA respectively. LshCas13a, on the other hand, uses only a short...(RPA) or Reverse-transcription RPA (RT-RPA), respectively. Amplified nucleotides are combined with Cas...expression. Although the collateral RNA cleavage response is well described in bacterial cells, LwaCas13a...