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We narrowed to 557 results for: ESP

Showing: 101 - 120 of 557 results

  1. Supporting Rare Disease Research with the 2020 BeHEARD Awards

    Type
    Blog Post
    Published
    Sept. 8, 2020, 2 p.m.
    ...to a large family of solute channels that are responsible for maintaining the nutrient and ionic balance...of an autoinflammatory loop: keratinocytes both respond to and release IL-36 cytokines. Keratinocytes are...cell and translate those signals into cellular responses. For example, GPCRs and their downstream G protein...

  2. Troubleshooting Your Plasmid Cloning Experiment

    Type
    Blog Post
    Published
    Sept. 24, 2019, 12:55 p.m.
    ...always a good idea to perform primer optimization, especially if you are having difficulty amplifying your ...colonies, check that the antibiotic in the plate correspond to the antibiotic resistance marker present in...small culture volume (2 or 3 ml) containing the corresponding antibiotic, extract the plasmid, and analyze...

  3. Channelrhodopsins with improved light sensitivity for minimally-invasive optogenetics

    Type
    Blog Post
    Published
    Nov. 3, 2020, 2:15 p.m.
    ... as channelrhodopsins (ChRs) makes them light-responsive. When light shines on excitatory channelrhodopsin-expressing...channelrhodopsin-expressing neurons, the channelrhodopsins respond by opening and allowing an influx of ions into ...expressing mice did not have this self-stimulation response. This result showed that ChRger2 stronger photocurrent...

  4. Developing Lab Management Software for Biology

    Type
    Blog Post
    Published
    Aug. 25, 2016, 2:30 p.m.
    ...everything. If it’s barcoded, it should have a corresponding entry in your database. Enjoy your happy lab...at some point, decide early on what to do in a namespace collision. It may even be worth it to have extra... you're solving, but consistency is key. Some namespace collisions you may encounter: vector, sequence...

  5. Plasmids 101: Cre-lox

    Type
    Blog Post
    Published
    Jan. 13, 2015, 3:47 p.m.
    ...cyclization recombinase”), is a 38 kDa protein responsible for intra- and inter-molecular recombination ...lox system is a well-established research tool, especially in the field of mouse transgenics. A few of the... (FRT) site being analogous to Cre and loxP, respectively. The FLP-FRT technology can be an effective ...

  6. Adeno Associated Virus (AAV) for Cell and Gene Therapy

    Type
    Blog Post
    Published
    Nov. 7, 2017, 1:59 p.m.
    ...lack all viral genes, including those that are responsible for integration into host chromosomes, further... (HDR). This characteristic makes AAV vectors especially amenable to correcting disease-associated mutations...PMCID: PMC4450362. 4. Sun, J. Y., et al. "Immune responses to adeno-associated virus and its recombinant ...

  7. Adenoviral Vector Production and Troubleshooting

    Type
    Blog Post
    Published
    June 14, 2018, 11:44 a.m.
    ... using AdV is that it triggers a strong immune response after in vivo administration, which results in...expression ceases relatively quickly due to immune response against AdV. Importantly, recombination-competent...spectrophotometry. An absorbance of at 260nm corresponds to 1.1 x 1012 particles/mL. To calculate the ...

  8. Cpf1 Update: Comparison to Cas9 and NgAgo

    Type
    Blog Post
    Published
    July 14, 2016, 2:30 p.m.
    ... and AsCpf1 are 3.7 kb and 3.9 kb in length, respectively, larger than SaCas9 but smaller than SpCas9....These nucleases should fit well into AAV vectors, especially given that Cpf1 gRNAs are also shorter than those...rate is similar to that of high fidelity Cas9s eSpCas9 and SpCas9-HF1. Both AsCpf1 and LbCpf1 ribonucleoproteins...

  9. Gaining Leadership Skills Volunteering at a Professional Organization

    Type
    Blog Post
    Published
    Aug. 6, 2015, 2:30 p.m.
    ...communication and learning to work with people despite the distance improved my verbal and written communication...strengths and work effectively. Leadership responsibilities Being a leader in an organization, you need... different hats and came to understand the responsibilities that come with leadership. The NPA was a great...

  10. PRIDICT: Predicting Efficiencies of Prime Editing Guide RNAs

    Type
    Blog Post
    Published
    June 25, 2024, 1:15 p.m.
    ...with the Krauthammer lab (University of Zurich), especially with my deep learning-wizard colleague Ahmed ...selecting the most promising pegRNA designs and especially facilitates the use of prime editing in pooled...true for any prime editor experimentalist, and especially shines once large-scale prime editing experiments...

  11. Transferable Skills: Negotiation

    Type
    Blog Post
    Published
    March 3, 2022, 2 p.m.
    ... on which lab jobs needed to be done (Who is responsible for making the running buffer this week?). Scientists...what is most important to each person. It can especially be difficult to think creatively when you are...create a win-win situation? Learning about and respecting cultural differences is a crucial part of understanding...

  12. No Llamas Required - Synthetic Nanobodies Against Membrane Proteins

    Type
    Blog Post
    Published
    June 18, 2020, 1:15 p.m.
    ...expensive to gain access to routinely, and it can be especially challenging when trying to develop antibodies... a defined set of different trinucleotides (corresponding to different amino acids) in the regions that...further characterization and analysis. In a rapid response to the COVID-19 pandemic, the Seeger lab demonstrated...

  13. Chromoproteins: Colorful Proteins For Molecular Biology Experiments

    Type
    Blog Post
    Published
    Feb. 4, 2020, 2:15 p.m.
    ...fluorescent proteins, it’s important to note that despite their distinction by name, chromoproteins are actually...pTOX6 express the YhaV, MqsR, and Tse2 toxins, respectively, and all encode the amilCP chromoprotein. Vectors...pTOX9 express the YhaV, MqsR, and Tse2 toxins, respectively, and encode the tsPurple chromoprotein.  Find...

  14. xCas9: Engineering a CRISPR Variant with PAM Flexibility

    Type
    Blog Post
    Published
    March 28, 2018, 6:52 p.m.
    ...there’s a PAM near your insertion site, the corresponding gRNA might be undesirable due to potential off-target...than SpCas9, in this case, NNGRRT and NNNNGATT, respectively. Researchers also engineered SpCas9 variants..., resulting in lower overall genome frequency. Despite the progress we’ve made, there is still a need ...

  15. Hot Plasmids: Summer 2025

    Type
    Blog Post
    Published
    Aug. 28, 2025, 1:15 p.m.
    ... the spotlight. TnpBs are gaining popularity, especially among plant biologists, due to their compact ...Pre-existing stem cell heterogeneity dictates clonal responses to the acquisition of leukemic driver mutations... ex vivo cell engineering applications and is especially well-suited for lung cells via airway administration...

  16. AAVs in Retinal Gene Therapy

    Type
    Blog Post
    Published
    July 31, 2018, 12:55 p.m.
    ...without triggering a destructive inflammatory immune response. At the time, adenoviral vectors were the most...disease-causing genes, including the RPE65 gene responsible for an autosomal recessive, severe, childhood-onset...follow in the footsteps of Luxturna™. However, despite promising results in preclinical studies some potential...

  17. AAVs CREATed for Gene Delivery to the CNS and PNS

    Type
    Blog Post
    Published
    Sept. 28, 2017, 2:01 p.m.
    ...are also an important target for gene delivery, especially for the study of pain. While many new capsids...and AAV.PHP.S, that target the CNS or the PNS, respectively.  Read on to learn more about these two new ...Cre-dependent selection yields AAV variants for widespread gene transfer to the adult brain. Nature biotechnology...

  18. Better Dyeing Through Chemistry & Small Molecule Fluorophores

    Type
    Blog Post
    Published
    Sept. 8, 2016, 2:30 p.m.
    ...individual molecules. Poor photon budgets are a widespread issue with fluorescent proteins—even the thriftiest...student searching couch cushions for spare change, desperate to extract a few more photons from a sample. Chemical...have excellent properties inside living cells, especially for advanced imaging experiments. We continue...

  19. Interview: Hodaka Fujii on enChIP, New CRISPR Tools, and More

    Type
    Blog Post
    Published
    Dec. 2, 2014, 7:23 p.m.
    ...and it would have been very difficult for us to respond to such a large number of requests by ourselves...with each other, which is critically important, especially for young researchers. It was a really nice incubator...Identification of Proteins Associated with an IFNgamma-Responsive Promoter by a Retroviral Expression System for...

  20. Plasmids 101: Stringent Regulation of Replication

    Type
    Blog Post
    Published
    June 20, 2024, 1:15 p.m.
    ...Quiñones-Valles, C., Espíndola-Serna, L., Martínez-Antonio, A., Quiñones-Valles, C., Espíndola-Serna, L., & Martínez-Antonio...Solar, G., Giraldo, R., Ruiz-Echevarría, M. J., Espinosa, M., & Díaz-Orejas, R. (1998). Replication and...
Showing: 101 - 120 of 557 results