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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 354 results for: HRE

Showing: 101 - 120 of 354 results

  3. Hot Plasmids: Spring 2025

    Type
    Blog Post
    Published
    June 3, 2025, 1:15 p.m.
    .../F0, Time-to-peak, Rising τ, and Decay τ of the three sensors. Image adapted from Aggarwal et al. 2025... highly-specific recombinant antibodies against three of the six human glypican proteins (GPC1, GPC3, ...

  4. CRISPR 101: Off-Target Effects

    Type
    Blog Post
    Published
    Feb. 8, 2024, 2:15 p.m.
    ...to ensure off-target events are below a given threshold for certain experiments where they can’t be tolerated...or maybe you just can’t get them below a given threshold even when you’ve tried everything listed here....

  5. Viral Vectors 101: AAV Variables That Matter

    Type
    Blog Post
    Published
    June 1, 2023, 1:15 p.m.
    ...ready to start selecting, testing, and optimizing three key variables of an AAV vector: serotype, promoter..., & Burke, R. E. (2016). Expression mediated by three partial sequences of the human tyrosine hydroxylase...

  6. Is this the right place for me? 8 tactics for choosing a lab

    Type
    Blog Post
    Published
    Oct. 2, 2018, 12:56 p.m.
    ...Academic Mentors.  What is workplace bullying? Threats to professional status – including belittling opinions...intimidating use of discipline or competence procedures Threats to personal standing – including undermining personal...

  8. Deep Dive: Statistical Tests (Comparisons)

    Type
    Blog Post
    Published
    March 7, 2023, 2:15 p.m.
    ... for more variables and groups. ANOVAs can test three or more groups (two samples and a control, for example...Bonferroni does this by establishing a significance threshold at a p value less than or equal to the alpha value...

  9. Important Considerations When Using AAVs

    Type
    Blog Post
    Published
    June 13, 2017, 2:30 p.m.
    ... real estate and using AAVs have in common? The three most important items when looking for a house and...Applying this mantra to AAV selection, there are three important factors related to location. Location...

  11. A Guide to Getting Started in Undergrad Research

    Type
    Blog Post
    Published
    June 8, 2021, 1:15 p.m.
    ... diseases. Others were inspired by the imminent threat of climate change to study plant and marine biology...Chrousos GP, Mentis A-FA (2020) Imposter syndrome threatens diversity. Science 367:749.2-750 . https://doi.org...

  13. Typing CRISPR Systems

    Type
    Blog Post
    Published
    March 18, 2025, 1:15 p.m.
    ... types — weird and poorly understood. There are three subtypes, A–C, and they are grouped together because...when introducing CRISPR as a technology. There are three subtypes, A–C, with the beloved SpCas9 (from Streptococcus...

  15. With an Eye Towards the Future, We Look Back at the March for Science

    Type
    Blog Post
    Published
    Oct. 24, 2017, 1:54 p.m.
    ...one person vocally protesting us, he was not threatening in any way. Participating in this march proved...policies that benefit polluting industries.In March, three climate scientists and an economist testified before...

  16. CRISPR 101: Cytosine Transversion Editors

    Type
    Blog Post
    Published
    March 25, 2025, 1:15 p.m.
    ...Mycobacterium smegmatis, termed UdgX, they found three proteins whose fusion improved the CGBE: DNA polymerase...where we’ll tackle transversion editors for the three other DNA bases, bringing the dream of any-base ...

  17. A Needle in a Base-Stack: Cas9 Structural Biology

    Type
    Blog Post
    Published
    June 4, 2024, 1:15 p.m.
    ...random binding to precision gene editing requires three major steps: RNA loading, DNA sequence recognition...DNA strand are not resolved due to disorder. All three stem loops and all 20 guide bases of the RNA are...

  18. CRISPR 101: Multiplex Expression of gRNAs

    Type
    Blog Post
    Published
    Sept. 10, 2020, 11:45 a.m.
    ... vectors expressing wt humanized Cas9 and up to three gRNAs. Entry vectors containing the U6 promoter ...fragments are PCR amplified for cloning into one of three Zeamays codon-optimized Cas9-containing binary vectors...

  19. CRISPR 101: Cytosine and Adenine Base Editors

    Type
    Blog Post
    Published
    Feb. 13, 2025, 2:15 p.m.
    ... editing work? Base transition editing requires three elements. They are, broadly: A Cas protein (catalytically...Cas9n-UGI linkers to improve product purity, and these three improvements represent the fourth generation of ...

  20. 15 Hot Plasmids from 2017

    Type
    Blog Post
    Published
    Jan. 9, 2018, 3:02 p.m.
    ...fluorescence, brightness, and proper maturation. Three new RFPs were isolated and characterized: mScarlet...lifetime of 1.3 ns, quantum yield of 0.20). All three mScarlet variants have shown great performance in...
Showing: 101 - 120 of 354 results