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Showing: 101 - 120 of 327 results

  1. Fluorescent Proteins 101: Luciferase

    Type
    Blog Post
    Published
    June 24, 2014, 3:59 p.m.
    ...peak emission at 560nm. Shortly after the initial article describing the cloning of firefly luciferase was...your tips below! Read More: All Plasmids 101 Articles Browse plasmids that contain luciferase ...

  2. 15 Years of Addgene: The Top 15 Plasmids

    Type
    Blog Post
    Published
    Jan. 8, 2019, 1:54 p.m.
    ... for producing lentiviral and MuLV retroviral particles. It can be used with packaging plasmids such as...integration needed for the production of lentiviral particles. It’s capable of inducible expression of shRNA...

  3. Hot Plasmids - May 2022

    Type
    Blog Post
    Published
    May 26, 2022, 1:15 p.m.
    ...the repository through our hot plasmids articles. These articles provide brief summaries of recent plasmid...

  4. Building and Validating Recombinant Antibodies: A Case Study

    Type
    Blog Post
    Published
    April 5, 2022, 1:20 p.m.
    ...to make the sequences of monoclonal antibodies publicly available. The Neuro Mab Sequencing Initiative...Trimmer lab hybridoma collection and make them publicly available. The lab has also expanded their recombinant...

  5. Hot Plasmids - February 2022

    Type
    Blog Post
    Published
    Feb. 22, 2022, 2:15 p.m.
    ...the repository through our hot plasmids articles. These articles provide brief summaries of recent plasmid...

  6. Viral Vectors 101: Biosensors

    Type
    Blog Post
    Published
    July 6, 2023, 1 p.m.
    ...determine viral tropism—the type of cells the viral particles infect. This can direct your biosensor to specific...choose lentivirus, gamma retrovirus, or AAV as your vehicle, we’ve got you covered with packaging plasmids!...

  7. Viral Vectors 101: Types of viruses

    Type
    Blog Post
    Published
    June 6, 2023, 1:15 p.m.
    ...infect both dividing and non-dividing cells. Lenti particles enter the nucleus through nuclear pores and thus...Relative to the other viruses discussed in this article, adenovirus has the highest maximum titer, a plus...

  8. RNA Extraction Without A Kit

    Type
    Blog Post
    Published
    March 24, 2020, 1:15 p.m.
    ...solutions when all the spin columns run out. In this article, we cover the basics of isolating RNA without a...is to precipitate the RNA using lithium chloride. LiCl solutions selectively precipitate RNA, but not DNA...

  9. Summer SciComm Series: Cognitive Load

    Type
    Blog Post
    Published
    June 16, 2022, 1:15 p.m.
    ...have the words to focus on. If you’re reading an article online, you have the words and maybe a few links...vying for your attention. If you’re reading an article with images, now you have words, images, and links...

  10. Transferable Skills Guide: Problem Solving

    Type
    Blog Post
    Published
    Nov. 30, 2021, 2:15 p.m.
    ...transferable skills seemed fuzzy or ill-defined. In this article, I try to clarify what people mean when they ask... After sifting through dozens of career advice articles, I settled on defining problem solving skills ...

  12. Uncertainty about Labor Law Brings More Uncertainty to Postdoc Wages

    Type
    Blog Post
    Published
    Jan. 11, 2017, 3:30 p.m.
    ...were at institutions where no data had been made publicly available (below). However, on November 22nd a...Resources on the Addgene Blog Read Gary's Previous Article: Changing Labor Laws Bring Increased Postdoc Wages...

  13. Hot Plasmids - October 2020

    Type
    Blog Post
    Published
    Oct. 13, 2020, 1:15 p.m.
    ...the repository through our hot plasmids articles. These articles provide brief summaries of recent plasmid...

  14. A Look Back at One Year of Plasmid Sharing for COVID-19 Research

    Type
    Blog Post
    Published
    July 12, 2021, 1:15 p.m.
    ...You can learn more about their research in this article. To see other plasmids for SARS-CoV-2 spike pseudotyping...Protocol and Reagents for Pseudotyping Lentiviral Particles with SARS-CoV-2 Spike Protein for Neutralization...

  15. Hot Plasmids and Viral Preps - March 2021

    Type
    Blog Post
    Published
    March 23, 2021, 1:15 p.m.
    ...the repository through our hot plasmids articles. These articles provide brief summaries of recent plasmid...

  16. Viral Vectors 101: Chemogenetics

    Type
    Blog Post
    Published
    July 11, 2023, 1:15 p.m.
    ... and some, such as varenicline, are already approved for clinical use. Varenicline has been further modified...

  17. Popular Retroviral Vectors and Their Uses in Scientific Research

    Type
    Blog Post
    Published
    July 24, 2018, 12:51 p.m.
    ...Viruses are intracellular parasites and natural vehicles for genetic information. Therefore they make excellent...produce a ~10-fold higher titer of lentiviral particles than the original lentiCRISPR v1. Special note...

  18. Adeno Associated Virus (AAV) for Cell and Gene Therapy

    Type
    Blog Post
    Published
    Nov. 7, 2017, 1:59 p.m.
    ...adenovirus, and inorganic delivery tools such as nanoparticles. These technologies can have serious adverse...popularity as they are uniquely suited gene delivery vehicles with great safety profiles as well as other benefits...

  19. Tips for Technical Support Calls

    Type
    Blog Post
    Published
    Sept. 20, 2016, 2:39 p.m.
    ...thousands of depositors. That means their original articles are your best bet to figure out how to use a particular...using Google Scholar, which searches scientific articles, patents, and even some doctoral dissertations...

  20. A Guide to Designing a Scientific Poster: Content, Layout, and More

    Type
    Blog Post
    Published
    Oct. 22, 2019, 12:20 p.m.
    ...contains the same sections as a primary research article, it’s important to understand that presenting your...Posters should not look like a primary research article condensed into one big page. Your sections should...
Showing: 101 - 120 of 327 results