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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 316 results for: Mut

Showing: 101 - 120 of 316 results

  1. Easi-CRISPR: Generating Knock-In and Conditional Mouse Models

    Type
    Blog Post
    Published
    April 5, 2018, 12:42 p.m.
    ...first generate your desired mutation in mouse ES cells, select for the mutation and inject the ES cells into... models. For simple knockout alleles or point mutations, the 8-10 month process of conventional gene targeting...PAM; if not, you can add a silent PAM or guide mutation. Designing an Easi-CRISPR conditional allele The...

  2. CRISPR 101: Multiplex Expression of gRNAs

    Type
    Blog Post
    Published
    Sept. 10, 2020, 11:45 a.m.
    ... are available: wt humanized Cas9, D10A nickase mutant (Cas9n), dCas9 (transcriptional repression), and...recombineering, with 96-99% efficiency for point mutations and 66% efficiency for small insertions. Multiplexing...degradation tag (LVA tag) to achieve specific point mutations in E. coli. gRNAs are delivered on a different.... Yin, Linlin, et al. “Multiplex Conditional Mutagenesis Using Transgenic Expression of Cas9 and sgRNAs...

  3. Keeping Up With CRISPR/Cas9

    Type
    Blog Post
    Published
    March 11, 2014, 5:55 p.m.
    ...that CRISPR's could be used to correct disease mutations, both in mice and in human cells. But that important...genes in one step without producing off-target mutations," Sha was quoted as saying when the news first...blogger Caroline LaManna put it well: "Wow, custom mutations in monkeys already. I know we've discussed it ...

  4. Optogenetics + CRISPR, Using Light to Control Genome Editing

    Type
    Blog Post
    Published
    Sept. 3, 2020, 12:15 p.m.
    ...by blue light (470nm), paCas9-1 induced indel mutations via NHEJ (frequency of 20.5%) and induced modifications...significantly alter the system’s efficiency at generating mutations when activated with light and lowered background...Hemphill et al. demonstrated that the Cas9 K866PCK mutant was indeed inactive prior to irradiation with UV...., 2015   Nitrobenzyl-caged lysine Cas9 K866PCK mutant Genome editing by NHEJ and HDR Jain et al., 2016...

  5. CRISPR Methods for Bacteria: Genome Engineering, CRISPRa, CRISPRi, Base Editing, and More

    Type
    Blog Post
    Published
    Sept. 28, 2020, noon
    ...non-edited cells to survive have inactivating mutations in the Cas9 or spacer sequence, and these rare...pneumoniae and can be used to generate multiple mutations simultaneously (Jiang et al. 2013). Genome editing...recombineering, with 96-99% efficiency for point mutations and 66% efficiency for small insertions. Multiplexing...they were effectively able to introduce point mutations in P. aeruginosa, Pseudomonas putida, Pseudomonas...

  6. Choosing Your Perfect Empty Backbone

    Type
    Blog Post
    Published
    Aug. 19, 2014, 3:39 p.m.
    ...wild-type version of YGOI to rescue a mutated strain or a mutated version of YGOI to test the different...different phenotypes of the specific mutations. For these purposes Addgene has a large collection of yeast expression...

  7. Bright Monomeric Fluorescent Proteins: mNeonGreen, mTFP1, and mWasabi

    Type
    Blog Post
    Published
    April 25, 2019, 3:01 p.m.
    ...-breaking mutations. The library was then screened through successive rounds of mutagenesis for the brightest...scientists from Florida State University, they used a mutagenesis approach and found that specific amino acid changes...

  10. Plasmids 101: Origin of Replication

    Type
    Blog Post
    Published
    Nov. 11, 2020, 2:23 p.m.
    ...negative regulation and can be manipulated with mutations in the replicon. For example, the pMB1 ori maintains...per cell, while pUC – which differs by only two mutations – will produce as many as 700 copies per cell....for single- and multiple-unnatural amino acid mutagenesis in Escherichia coli. Biochemistry. 2013 Mar 12...

  11. 15 Years of Addgene: The Top 15 Plasmids

    Type
    Blog Post
    Published
    Jan. 8, 2019, 1:54 p.m.
    ...plasmid of all time, but was found to have a point mutation in the puromycin cassette that makes it less efficient...Cas9, this plasmid expresses Cas9n (a D10A nickase mutant) to nick, rather than cleave DNA. Find pSpCas9n...(BB)-2A-Puro (PX459) V2.0. It contains a point mutation in the puromycin cassette that makes it less efficient...

  12. Hot Plasmids December 2018 - C. elegans RNAi, TurboID, Detecting Viral Outbreaks, and Protein Binding Specificity Tools

    Type
    Blog Post
    Published
    Dec. 6, 2018, 1:34 p.m.
    ...to study. To overcome this, most scientists use mutant animals that are genetically altered to overexpress...overexpress endogenous RNAi pathways. These mutations however can interfere with other genetic pathways making...than standard RNAi protocols without the need for mutant genetic backgrounds. The standard RNAi vector, ...

  13. Back to Bacteria: CRISPR gRNA Multiplexing Using tRNAs

    Type
    Blog Post
    Published
    June 2, 2015, 6:06 p.m.
    ...vivo and in vitro, as well as to insert targeted mutations through homologous recombination. To further increase...protoplasts, Xie et. al observed slightly higher mutation rates than they’d previously reported in protoplasts..., they found that 50% of plants had biallelic mutations at all five loci. This highly efficient multiplexing...

  15. Addgene's Tips for Plasmid Quality Control

    Type
    Blog Post
    Published
    Jan. 14, 2016, 3:30 p.m.
    ...plasmid are the backbone, inserted gene/functional mutations, antibiotic resistance, restriction sites used...most sequence assembly errors occur), deletions, mutations, in-frame protein fusions, and multiple cloning...cases, we deal with multiple inserts or internal mutations that require more than two sequencing reactions...

  16. Hot Plasmids February 2024

    Type
    Blog Post
    Published
    Feb. 20, 2024, 2:45 p.m.
    ... generated a series of StayGold mutants and identified a triple mutant, Y187F, R144I and T155Q, which ...Balasubramanian and colleagues showed the single mutation E138D is sufficient to disrupt dimerization while...

  17. A Look at Addgene's QC Process

    Type
    Blog Post
    Published
    May 6, 2025, 1:15 p.m.
    ... function of the plasmid. This includes point mutations, frame shifts, missing elements, and nucleotide...plasmid that are less likely to affect function when mutated; elements that are difficult to sequence; approaches...Sequence discrepancies are not always a result of mutations in the plasmid, but can arise due to errors in...

  18. CRISPR 101: Targeting Non-Coding RNAs with CRISPR/Cas9

    Type
    Blog Post
    Published
    Nov. 14, 2024, 2:15 p.m.
    ...the majority of the lncRNA locus, scientists can mutate the transcription start site, splice sites, exons...function (Hazan and Bester 2021; Zibitt et al. 2021). Mutating splice sites is particularly effective as it can... Transcription start site (TSS) deletion. (B) Mutations of splice sites. (C) Removal of an exon or a large...

  19. Hot Plasmids: Summer 2025

    Type
    Blog Post
    Published
    Aug. 28, 2025, 1:15 p.m.
    ... during differentiation after they introduced mutations that drive leukemia (Figure 3). Figure...useful for studying the impacts of other cancer mutations and non-genetic diversity in human cancer stem...responses to the acquisition of leukemic driver mutations. Cell Stem Cell, 32(4), 564–580.e6. https://doi.org...

  20. Fluorescent Proteins 101: Fluorescent Protein Timers

    Type
    Blog Post
    Published
    May 4, 2017, 2:30 p.m.
    ...knowledge of protein structure and saturation mutagenesis. mK-GO matures from green to red, and the FT ...absorption spectra). Subach et al’s directed mutagenesis also produced the three separate timers shown...3. Verkhusha, Vladislav V., et al. "An enhanced mutant of red fluorescent protein DsRed for double labeling...
Showing: 101 - 120 of 316 results