We narrowed to 221 results for: PORA

1. Choosing Your Perfect Empty Backbone

   Type

   Blog Post

   Published

   Aug. 19, 2014, 3:39 p.m.

   ...have to make sure that your plasmid has been incorporated into the host organism, usually achieved with...

2. History of CRISPR Cas - A tale of survival and evolution

   Type

   Blog Post

   Published

   Nov. 25, 2013, 10:12 p.m.

   ...phages to test if the exogenous DNA would be incorporated into the bacterial genome. Cas genes, which ...

3. Viral Vectors 101: Transductions

   Type

   Blog Post

   Published

   April 30, 2024, 1:15 p.m.

   ...nature. All three of these methods involve DNA incorporation into the cell, but the mechanisms vary. Why ...

4. A New Optogenetic Tool Based on AraC Controls Gene Expression with Blue Light

   Type

   Blog Post

   Published

   June 17, 2021, 1:17 p.m.

   ...transcription.However, this system lacks spatio-temporal regulation, and is difficult to reverse, making...

5. Summer SciComm Series: Master of SciComm

   Type

   Blog Post

   Published

   July 21, 2022, 1:15 p.m.

   ...environment. Over time, this interest came to incorporate other facets which occupied my attention, until...

6. Viral Genome Sequencing - A One Stop Shop for AAV Identity and Serotype Confirmation

   Type

   Blog Post

   Published

   April 23, 2020, 1:15 p.m.

   ...sequencing and analysis approach can be easily incorporated into standard QC regimens and will provide scientists...

7. New and Upcoming Viral Vectors - December 2019

   Type

   Blog Post

   Published

   Dec. 19, 2019, 2:40 p.m.

   ...Li Lab. These GPCR based sensors provide the spatiotemporal resolution required for precise in vivo measurements...

8. Why Add Sucrose? Improved Yields for Adeno-associated Virus Preparation

   Type

   Blog Post

   Published

   Dec. 13, 2018, 1:14 p.m.

   ...new tools and protocols that they can easily incorporate into their own labs. This week we are pleased...

9. CRISPR Challenges: Standardization and Homology Directed Repair

   Type

   Blog Post

   Published

   Feb. 14, 2018, 2:36 p.m.

   ...strand annealing (SDSA) and single-strand DNA incorporation (ssDI). "Easi-CRISPR for creating knock-in and...

10. In Vivo Biotinylation of Bacterial Fusion Proteins

    Type

    Blog Post

    Published

    Jan. 25, 2018, 2:09 p.m.

    ...acids. Unfortunately, the location of biotin incorporation and to a lesser extent, the number of added ...

11. What Do I Do Now? Academic v. Non-Academic Career Decisions

    Type

    Blog Post

    Published

    March 9, 2017, 3:40 p.m.

    ...cushy”—tenured faculty position for the grind of corporate life”.   In fact, there are a lot of good reasons...

12. 10 Great Guest Posts We're Thankful For

    Type

    Blog Post

    Published

    Nov. 18, 2016, 3 p.m.

    ...potentially be used to report upon and/or engineer “temporally ordered biochemical events” such as those that...

13. Extracurricular Activities for a Strong Science Career Path

    Type

    Blog Post

    Published

    Jan. 21, 2014, 3:08 p.m.

    ...highlight the importance of taking a break and incorporating EAs into your routine.  Getting started: Planning...

14. Visualizing Genomic Loci with CRISPR-Sirius

    Type

    Blog Post

    Published

    April 18, 2019, 12:53 p.m.

    ...robust visualization tool to study the spatial and temporal arrangement of chromosomes and how these arrangements...

15. Creating accessible biology activities in schools with BioBits

    Type

    Blog Post

    Published

    Aug. 2, 2018, 12:56 p.m.

    ...encodes it. That DNA sequence then needs to be incorporated into a host cell (through a process called transformation...

16. Learning Technical Skills at a Lab Central Workshop

    Type

    Blog Post

    Published

    Feb. 20, 2018, 2:30 p.m.

    ...colors of orange and white. The decor was very contemporary and futuristic with block chairs in the hallway...

17. Pushing the Limits of DNA Assembly

    Type

    Blog Post

    Published

    Sept. 6, 2017, 1:01 p.m.

    ...assembly. What makes it so forgiving, is the incorporation of a 3’-5’ exonuclease in the reaction mixture...

18. Fluorescent Protein Travel Awards - FLiPs and Fluorescent Protein Biosensors

    Type

    Blog Post

    Published

    July 27, 2017, 1:11 p.m.

    ...particularly notes their ability to be precisely spatio-temporally controlled as a key advantage over other inducible...

19. Quick Guide to Working with Drosophila Part 2: Controlling Gene Expression in Flies with Gal4/UAS

    Type

    Blog Post

    Published

    July 21, 2017, 12:48 p.m.

    ...you to systematically study gene function with temporal control and cell-type specificity! The Gal4/...

20. Optimizing Donor DNA for Enhanced CRISPR Genome Editing

    Type

    Blog Post

    Published

    March 24, 2016, 2:30 p.m.

    ... and the targeting guide RNA into cells by electroporation. Results from our lab and others (Lin et al...