We narrowed to 289 results for: Pol
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TypeBlog PostPublishedJan. 15, 2019, 2:19 p.m....walking tour of Buddy the Elf’s journey from the North Pole to New York City. Check out their video below. ...interpretation of Buddy the Elf's journey from the North Pole to New York. #ELF #DeckTheLab #ProudPIhttps://t....
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Troubleshooting and Optimizing a Western Blot
TypeBlog PostPublishedSept. 17, 2024, 1:15 p.m....biorender.com. Since antibodies, especially polyclonal antibodies, can vary lot-to-lot, this range needs...this starting point. You can also consider using polyclonal or recombinant multiclonal antibodies to increase... time Not enough antibody binding sites Polyclonal or recombinant multiclonal antibodies Protein... -
Future of Research Conference - Remarkable Opening Session
TypeBlog PostPublishedOct. 7, 2014, 3 p.m....amazing postdocs, Kristin Krukenberg and Jessica Polka from Harvard Medical School and a long list of supporters...message delivered by many of the speakers. As Dr. Polka said as she started things off, “We are all capable... -
Celebrating Outstanding CRISPR/Cas9 Achievements at the Dr. Paul Janssen Award Dinner
TypeBlog PostPublishedSept. 15, 2014, 6:05 p.m....Krzysztof Chylinski (who coincidentally speak the same Polish sub-dialect making their Skype communications extra...evolution A Powerful New Way to Edit DNA (by Andrew Pollack of New York Times) ... -
Management for Scientists: What Makes a Good Manager Anyway?
TypeBlog PostPublishedDec. 9, 2014, 2:22 p.m....include training or teaching others, research, and policy development. Every worker at every level does better...all times when making decisions, assignments and polices. Sometimes the boss has to be the boss and make... -
Plasmids 101: Antibiotic Resistance Genes
TypeBlog PostPublishedJan. 30, 2014, 3:29 p.m....Bacteriostatic 50-100 ug/mL in EtOH Polymyxin B polypeptide Alters outer membrane permeability Bactericidal... -
History of CRISPR Cas - A tale of survival and evolution
TypeBlog PostPublishedNov. 25, 2013, 10:12 p.m.... bacterial genome. Cas genes, which code for polymerases, nucleases, and helicases, were also disrupted... into three distinct types and subtypes using polythetic classification based on evolutionary similarity... -
Hot Plasmids June 2018 - Reverse transcriptase, nanobody, and protein-DNA interaction tools
TypeBlog PostPublishedJune 27, 2018, 1:16 p.m.... low processivity (ie. the probability of the polymerase continuing to copy the template rather than dissociating...improvements to existing technologies based on polymerase processivity. Please find the MarathonRT plasmid... -
Behind-the-scenes of the Isolation of the Thermostable IgnaviCas9 From a Yellowstone Hot Spring
TypeBlog PostPublishedNov. 12, 2019, 2 p.m....previously yielded valuable enzymes. Famously, Taq polymerase – from Thermus aquaticus bacterium – was discovered...make them extremely dangerous, we used telescoping poles to collect water samples from a distance. At each... -
Antibodies 101: Epitope Availability
TypeBlog PostPublishedJan. 11, 2022, 2 p.m....one epitope. (It can sometimes be an issue with polyclonal antibodies, so it's good to keep epitope availability...Antibodies 101: Monoclonal Antibodies Antibodies 101: Polyclonal Antibodies Antibodies 101: Introduction to Antibodies... -
Three Tips for Preventing Viral Plasmid Recombination in Your Samples
TypeBlog PostPublishedSept. 14, 2021, 1:15 p.m....vectors (LTRs and ITRs), plasmids with multiple polyA sites, and other plasmids that contain repeating...J, Plank C, Aneja MK, Rudolph C (2019) Segmented poly(A) tails significantly reduce recombination of plasmid... -
Addgene Receives NIH BRAIN Initiative Grant to Create Open-Access Recombinant Antibody Resource
TypeBlog PostPublishedMay 25, 2021, 2:29 p.m....for antibody production have several drawbacks. Polyclonal antibodies produced in animals may be variable...potential for further engineering as compared to polyclonal or monoclonal antibodies. You can learn more ... -
10 Great Guest Posts We're Thankful For
TypeBlog PostPublishedNov. 18, 2016, 3 p.m....Membrane-Rupturing Microscopic Tools In this post, Jessica Polka, formerly a postdoctoral researcher in the Silver... other chemical fuel sources.” In her work, Dr. Polka made R-body mutants that respond to different pH... -
Addgene-Seeding Labs plasmid grant round 3: microbial drug resistance and biofilms, antidiabetic plant components, visualizing brain injury - and a glowing robot fish
TypeBlog PostPublishedNov. 27, 2018, 2 p.m....glowing bacteria and a fish robot to detect water pollution Dr. Julio Ernesto Valdivia Silva, Universidad ...related to space medicine. Now he aims on tracking pollution in rivers by employing synthetic biology resources... -
Pushing the Limits of DNA Assembly
TypeBlog PostPublishedSept. 6, 2017, 1:01 p.m....HiFi so accurate (apart from the high-fidelity polymerase) is the ability to remove, reset and ligate mismatched...primer design based on fragment sequences and the polymerase used in the amplification. This tool allows instant... -
Visualizing Translation at the Single Molecule Level
TypeBlog PostPublishedAug. 1, 2017, 1:15 p.m....and Oskar protein were detected at the posterior pole. Oskar protein levels were negatively correlated... translated, the SunTag sites on the nascent polypeptide recruit scFV-GFP, generating green puncta that... -
5 Great Apps for Lab Life
TypeBlog PostPublishedSept. 22, 2016, 2:30 p.m.... depending on the primer sequence and the NEB polymerase used. Overall, while much of the information ...allow you to write your colleagues’ names in a polypeptide chain, except if their names have a B, J, X, ... -
Plasmids 101: Mammalian Vectors
TypeBlog PostPublishedMarch 25, 2014, 3:15 p.m...., NIH3T3, CHO, 293HEK, Jurkat T cells Blocks polypeptide synthesis at 80S; inhibits chain elongation 100...hygB HeLa, NIH3T3, CHO, Jurkat T cells Blocks polypeptide synthesis at 80S; inhibits chain elongation. ... -
Antibodies 101: ChIP
TypeBlog PostPublishedMarch 10, 2022, 3:25 p.m....incubate the sample with your antibody. Both polyclonal and monoclonal antibodies can work well in ChIP...Antibodies 101: Immunoprecipitation Antibodies 101: Polyclonal Antibodies Antibodies 101: Introduction to Antibodies... -
The Many Reasons for Irreproducible Research- and a Vaccine to Eradicate It
TypeBlog PostPublishedJan. 30, 2020, 2:15 p.m....guest blogger David Mellor! David Mellor leads the policy and incentive programs at the Center for Open Science...reduced bias in scientific research. These include policies for publishers and funders in the Transparency...