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We narrowed to 468 results for: SEC

Showing: 101 - 120 of 468 results

  1. A Career in Grants: How To Become a Grant Professional

    Type
    Blog Post
    Published
    Aug. 20, 2015, 2:30 p.m.
    ... grant readiness, identify grant opportunities, secure grant awards, and manage grant projects and funds... She has served in the nonprofit and government sector for 20 years, developing expertise in grant project...She and her team at J. Miller & Associates have secured more than $70 million dollars in grant funding....

  2. Using Video to Share Your Science: We Share Science

    Type
    Blog Post
    Published
    Nov. 3, 2016, 2:30 p.m.
    ...primary target audience – colleagues in our field. Secondary audiences that may also benefit, such as researchers...research scientists, faculty, students, private sector researchers, or pretty much anyone applying the...funding, or are by researchers for whom English is a second or third language (making publishing their research...

  3. Changing Labor Laws Bring Increased Postdoc Wages

    Type
    Blog Post
    Published
    Oct. 25, 2016, 2:30 p.m.
    ...2014, the White House issued a memorandum to the Secretary of Labor to update the FLSA, which had not seen...automatically adjusted every 3 years. In May 2016, Secretary of Labor Thomas Perez updated the overtime regulations...post by Director of the NIH Francis Collins and Secretary of Labor Thomas Perez, Fair Pay for Postdocs: ...

  4. Bioinformatics at Addgene

    Type
    Blog Post
    Published
    July 14, 2022, 1:15 p.m.
    ...linking the output from the first process to the second process. For example, our bbduk process created... declaration correctly, the script block in the second process printed one of the inputs, such as the ...copied a few lines of our existing script into the section of the next process. Similar to our first attempt...

  5. Plasmids 101: The Promoter Region – Let's Go!

    Type
    Blog Post
    Published
    April 3, 2014, 8:05 p.m.
    ...Strong insect promoter from baculovirus Constitutive  Commonly used in expression systems for insect cells... promoter. Ac5 General expression mRNA Strong insect promoter from Drosophila Actin 5c gene Constitutive...

  6. CRISPR 101: Homology Directed Repair

    Type
    Blog Post
    Published
    Jan. 26, 2023, 2:15 p.m.
    ...central steps: The 5’ DNA end of the break is resected by nucleases to create a ssDNA 3’ overhang. This... strand “captures” and synthesizes DNA from the second DSB end. The individual HJs are resolved via cleavage...importance for the repair of breaks that lack a second end to enable DSBR or SDSA.   Homology directed...

  7. Adeno-associated Viruses (AAVs) for Genome Editing

    Type
    Blog Post
    Published
    March 27, 2018, 1:32 p.m.
    ...a gRNA in one AAV and a repair template in second a second AAV to deliver their repair template to mouse...are more appropriate for a CRISPR-based approach. Second, the AAV preps generally used for gene editing ...

  8. Deep Mutational Scanning with One Pot Saturation Mutagenesis

    Type
    Blog Post
    Published
    Feb. 22, 2017, 3:30 p.m.
    ...desired codon changes (step 1 in the image above). A second universal primer is used to synthesize a complementary...strand. Each degenerate primer set contains three consecutive randomized bases (NNN) at a given codon site ...ExoIII and ExoI. Synthesize 2nd mutant strand: The second mutant strand is synthesized in the same manner...

  9. Fluorescent Proteins 101: GFP Fusion Proteins - Making the Right Connection

    Type
    Blog Post
    Published
    April 9, 2019, 1:13 p.m.
    ...Joachim Goedart, an assistant professor at the Section of Molecular Cytology and van Leeuwenhoek Centre...best site for the insertion is a loop in between secondary structure (beta-sheets or alpha helices). An additional...Joachim Goedhart is an assistant professor at the Section of Molecular Cytology and Van Leeuwenhoek Centre...

  10. Multiple Plasmids at a Low Price: Kits from Addgene

    Type
    Blog Post
    Published
    Sept. 14, 2016, 2:30 p.m.
    ... questions, please write to us in the comments section.  FX cloning kit This kit is designed to be used...expression in bacteria, yeast, mammalian cells, insects, and even Xenopus oocytes. The expression vectors...Translation of the standardized leader peptide prevents secondary structures in the mRNA encoding your gene of interest...

  11. Using Phosphoserine to Study Protein Phosphorylation

    Type
    Blog Post
    Published
    June 23, 2016, 2:30 p.m.
    ...bacterial host strain for pSer-incorporation The second component necessary for phosphoserine incorporation...will allow full length GFP to be translated), and second, through a gel shift assay using the PhosTag system...Wisconsin. She is particularly interested in the intersection of cell signaling, metabolism, and the mighty...

  12. How to Negotiate a Successful Lab Start Up Budget

    Type
    Blog Post
    Published
    Sept. 1, 2015, 2:30 p.m.
    ... we suggest estimating the time it will take to secure your first (R-level) research grant.  Set your ...?” As scientists, working with this question is second nature. Channel your inner curiosity and use your... be afraid to walk away from a negotiation. If securing a faculty position is your goal, then a resource...

  13. Evolution of Brainbow: Using Cre-lox for Multicolor Labeling of Neurons

    Type
    Blog Post
    Published
    April 24, 2015, 2:39 p.m.
    ...diffusible labels, or electron microscopy of stained sections, were limited due to low resolution or were very...fluorophores to aggregate in the neurons’ somata. Second, the system did not permit analysis by immunostaining...labeling has decreased the mapping time for a given section of brain by at least an order of magnitude. It ...

  14. Scientific Reproducibility - Focusing on Solutions at the Minisymposium on Reproducibility

    Type
    Blog Post
    Published
    May 18, 2018, 7:32 p.m.
    ...different time stamps in the description to watch any sections you’re particularly interested in, but I’d recommend...technique simply by following the abbreviated methods section found within a published manuscript. Luckily, many...a long way to encouraging researchers to take a second and think about how their biases affect their experimental...

  15. Important Considerations When Using AAVs

    Type
    Blog Post
    Published
    June 13, 2017, 2:30 p.m.
    ...neurons. Your choice of AAV will depend upon which section you’re looking at (striatum, hippocampus and cortex...single stranded DNA viruses, AAVs normally require second strand synthesis before gene expression begins....PMID: 11509958. 8. Ferrari, Forrest K., et al. "Second-strand synthesis is a rate-limiting step for efficient...

  16. Custom CRISPR Screens & the Green Listed Software

    Type
    Blog Post
    Published
    July 11, 2017, 2:30 p.m.
    ...knowledge could be very useful for drug discovery, to dissect a complex signaling pathway, and to identify what... accelerate progression into clinical trials. Secondary screens  If you have generated a list of candidate...candidate genes from a full genome screen, a smaller, secondary, CRISPR screen could be used to validate the list...

  17. Fluorescent Proteins 101: Photoactivatable Fluorescent Proteins

    Type
    Blog Post
    Published
    April 25, 2017, 2:30 p.m.
    ...switch or photoconversion usually takes less than a second. The first successful irreversible PA-FP reported...activate the first PA-FP does not photobleach the second, and vice versa. Finally, as every microscope will...favorite use for PA-FPs? Let us know in the comments section below! Currently, there are over 20 different varieties...

  18. 27 Hot Plasmids from 2016

    Type
    Blog Post
    Published
    Dec. 22, 2016, 3:03 p.m.
    ...was cloned into the Liu lab’s own pAc-sgRNA-Cas9 insect expression backbone, which expresses the gRNA from...Toronto KnockOut (TKO) CRISPR Library. This complex second-generation CRISPR lentiviral library targets nearly...target ~15,000 genes with 6 gRNAs per gene. The second is a "supplementary" library that contains an additional...tet-inducible OsTIR1 plasmid is also available). Second, they co-transfected short-arm donor vectors containing...

  19. CRISPR Guide Update

    Type
    Blog Post
    Published
    Oct. 10, 2024, 1:15 p.m.
    ... disposal. With this update, we have added new sections to share and describe some incredible new CRISPR...Highlights include a much-anticipated prime editing section and additions describing CRISPR’s ability to make...

  20. Technical Design of a Western Blot

    Type
    Blog Post
    Published
    Aug. 22, 2024, 1:15 p.m.
    ... primary antibody overnight at 4 °C and their secondary antibody for 1–2 hrs at room temperature, which...providing flexibility in assay design. Conjugated secondary antibodies are relatively cheap and easy to source...: Selecting the Right AntibodyAntibodies 101: Secondary Antibodies Resources on Addgene.org Addgene's ...
Showing: 101 - 120 of 468 results