We narrowed to 478 results for: SEC

... grant readiness, identify grant opportunities, secure grant awards, and manage grant projects and funds... She has served in the nonprofit and government sector for 20 years, developing expertise in grant project...She and her team at J. Miller & Associates have secured more than $70 million dollars in grant funding....

...linking the output from the first process to the second process. For example, our bbduk process created... declaration correctly, the script block in the second process printed one of the inputs, such as the ...copied a few lines of our existing script into the section of the next process. Similar to our first attempt...

...primary target audience – colleagues in our field. Secondary audiences that may also benefit, such as researchers...research scientists, faculty, students, private sector researchers, or pretty much anyone applying the...funding, or are by researchers for whom English is a second or third language (making publishing their research...

...2014, the White House issued a memorandum to the Secretary of Labor to update the FLSA, which had not seen...automatically adjusted every 3 years. In May 2016, Secretary of Labor Thomas Perez updated the overtime regulations...post by Director of the NIH Francis Collins and Secretary of Labor Thomas Perez, Fair Pay for Postdocs: ...

...Strong insect promoter from baculovirus Constitutive  Commonly used in expression systems for insect cells... promoter. Ac5 General expression mRNA Strong insect promoter from Drosophila Actin 5c gene Constitutive...

...central steps: The 5’ DNA end of the break is resected by nucleases to create a ssDNA 3’ overhang. This... strand “captures” and synthesizes DNA from the second DSB end. The individual HJs are resolved via cleavage...importance for the repair of breaks that lack a second end to enable DSBR or SDSA.   Homology directed...

...Joachim Goedart, an assistant professor at the Section of Molecular Cytology and van Leeuwenhoek Centre...best site for the insertion is a loop in between secondary structure (beta-sheets or alpha helices). An additional...Joachim Goedhart is an assistant professor at the Section of Molecular Cytology and Van Leeuwenhoek Centre...

...desired codon changes (step 1 in the image above). A second universal primer is used to synthesize a complementary...strand. Each degenerate primer set contains three consecutive randomized bases (NNN) at a given codon site ...ExoIII and ExoI. Synthesize 2nd mutant strand: The second mutant strand is synthesized in the same manner...

...a gRNA in one AAV and a repair template in second a second AAV to deliver their repair template to mouse...are more appropriate for a CRISPR-based approach. Second, the AAV preps generally used for gene editing ...

... questions, please write to us in the comments section.  FX cloning kit This kit is designed to be used...expression in bacteria, yeast, mammalian cells, insects, and even Xenopus oocytes. The expression vectors...Translation of the standardized leader peptide prevents secondary structures in the mRNA encoding your gene of interest...

...bacterial host strain for pSer-incorporation The second component necessary for phosphoserine incorporation...will allow full length GFP to be translated), and second, through a gel shift assay using the PhosTag system...Wisconsin. She is particularly interested in the intersection of cell signaling, metabolism, and the mighty...

... we suggest estimating the time it will take to secure your first (R-level) research grant.  Set your ...?” As scientists, working with this question is second nature. Channel your inner curiosity and use your... be afraid to walk away from a negotiation. If securing a faculty position is your goal, then a resource...

...diffusible labels, or electron microscopy of stained sections, were limited due to low resolution or were very...fluorophores to aggregate in the neurons’ somata. Second, the system did not permit analysis by immunostaining...labeling has decreased the mapping time for a given section of brain by at least an order of magnitude. It ...

...different time stamps in the description to watch any sections you’re particularly interested in, but I’d recommend...technique simply by following the abbreviated methods section found within a published manuscript. Luckily, many...a long way to encouraging researchers to take a second and think about how their biases affect their experimental...

...neurons. Your choice of AAV will depend upon which section you’re looking at (striatum, hippocampus and cortex...single stranded DNA viruses, AAVs normally require second strand synthesis before gene expression begins....PMID: 11509958. 8. Ferrari, Forrest K., et al. "Second-strand synthesis is a rate-limiting step for efficient...

...switch or photoconversion usually takes less than a second. The first successful irreversible PA-FP reported...activate the first PA-FP does not photobleach the second, and vice versa. Finally, as every microscope will...favorite use for PA-FPs? Let us know in the comments section below! Currently, there are over 20 different varieties...

...knowledge could be very useful for drug discovery, to dissect a complex signaling pathway, and to identify what... accelerate progression into clinical trials. Secondary screens  If you have generated a list of candidate...candidate genes from a full genome screen, a smaller, secondary, CRISPR screen could be used to validate the list...

...was cloned into the Liu lab’s own pAc-sgRNA-Cas9 insect expression backbone, which expresses the gRNA from...Toronto KnockOut (TKO) CRISPR Library. This complex second-generation CRISPR lentiviral library targets nearly...target ~15,000 genes with 6 gRNAs per gene. The second is a "supplementary" library that contains an additional...tet-inducible OsTIR1 plasmid is also available). Second, they co-transfected short-arm donor vectors containing...

... disposal. With this update, we have added new sections to share and describe some incredible new CRISPR...Highlights include a much-anticipated prime editing section and additions describing CRISPR’s ability to make...

... primary antibody overnight at 4 °C and their secondary antibody for 1–2 hrs at room temperature, which...providing flexibility in assay design. Conjugated secondary antibodies are relatively cheap and easy to source...: Selecting the Right AntibodyAntibodies 101: Secondary Antibodies Resources on Addgene.org Addgene's ...